Passage of chromium-mordanted and rare earth-labeled fiber: time of dosing kinetics

Coastal bermudagrass hay was labeled with Cr by the Cr-mordant procedure and with 177Lu applied to the same fiber. Neutral detergent fiber prepared from the same Coastal bermudagrass hay was labeled with Yb, 169Yb, Tb and 160Tb by soaking overnight following by thorough washing and drying. Wood chips were similarly labeled with Sm or La, and Solka Floc was labeled with 147Nd and 141Ce. The carriers, labels and times of administration to cattle were: bermudagrass fiber with both Cr and 177Lu, bermudagrass fiber with 169Yb and Solka Floc labeled with 147Nd at 0 h; bermudagrass fiber with Yb, Solka Floc with 141Ce and wood chips with Sm at 24 h; wood chips with La at 48 h; and bermudagrass fiber labeled with 160Tb at the beginning and labeled with Tb at the end of a meal. Fecal collection followed and passage characteristics were determined with a two-compartment, age-dependent model. Markers labeling the different fiber sources had different (P less than .01) passage rates (Solka Floc greater than Coastal bermudagrass greater than wood chips), but there was no difference within fiber source for rare earth passage. There also was no difference between the passage characteristics of Cr-mordant and 177Lu. However, passage rate of particles administered at the beginning of the meal (160Tb) was 42% higher than for particles at the end of the meal (Tb).(ABSTRACT TRUNCATED AT 250 WORDS)     

Physiological effects of etorphine, acepromazine and xylazine in the scimitar horned oryx (Oryx dammah)

Scimitar horned oryx (Oryx dammah), kept under confined and unconfined conditions were immobilised with etorphine in combination with acepromazine or xylazine or both, and with xylazine alone. Both groups of animals were successfully sedated with etorphine and xylzine, with or without acepromazine, although hypothermia and mild hypoxaemia and a fall in packed cell volume were frequently noted. Xylazine alone produced a dose dependent degree of sedation in semitame subadult animals kept in confinement, but only slight depression in their wild, unconfined counterparts. If xylazine was not included in the immobilising mixture induction was traumatic and full sedation not achieved. Heart rates and arterial pressures (systemic and pulmonary) were also monitored but no remarkable changes were noted. The only abnormalities in blood biochemistry were raised aspartate transminase and creatine kinase. Ruminal regurgitation could be a major problem if endotracheal intubation was not achieved early in the procedure.     

Three cases of local tetanus

Unyielding rigidity of one thoracic limb due to continuous involuntary muscle spasm was seen as the presenting complaint in two cats and a dog. A distal forelimb injury on the affected limb preceded the development of clinical signs in two animals. No abnormalities were detected in a detailed histological examination of the first case. The continuous localised muscular spasm subsided completely over a prolonged period in the remaining two cases. These findings strongly suggest a diagnosis of local tetanus. The diagnosis and treatment of this rare condition are discussed.     

Is a Delayed Treatment with GnRH,hCG or Progesterone Beneficial for Reducing Embryonic Mortality in Buffaloes?

The aims of this study were to verify the efficacy of delayed hormonal treatments performed on day 25 post‐insemination on pregnancy rate at 45 and 70 days in buffalo. The trial was performed on 385 buffaloes synchronized by the Ovsynch/TAI protocol and submitted to artificial insemination (AI). Twenty‐five days after AI, pregnant animals were assigned to four treatments: (1) GnRH agonist (n = 52), 12 μg of buserelin acetate; (2) hCG (n = 51), 1500 IU of human chorionic gonadotrophin; (3) Progesterone (n = 47), 341 mg of P4 intramuscular (im) every 4 days for three times; (4) Control (n = 54), treatment with physiological saline (0.9% NaCl). Milk samples were collected on days 10, 20 and 25 after AI in all buffaloes to determine progesterone concentration in whey by radioimmunoassay method. Statistical analysis was performed by anova . Pregnancy rate on day 25 after AI was 52.9%, but declined to 41.8% by day 45, indicating an embryonic mortality (EM) of 21%. If only control group is considered, the incidence of EM was 38.9%. Pregnant buffaloes had higher (p < 0.01) progesterone concentrations on day 20 and 25 after AI than both non‐pregnant buffaloes and buffaloes that showed EM. The treatments on day 25 increased (p < 0.01) pregnancy rate, although in buffaloes with a low whey progesterone concentration on day 20 and 25 after AI (n = 22); all treatments were ineffective to reduce EM.     

Chaining and Burning Modifies Vegetation Structure,Fuel, and Post-Disturbance Sprouting Capacity

Prescribed fire and/or mechanical methods can be used to modify the quantity, continuity, and/or spatial arrangement of flammable fuel. Yet the consequences of fuel management, both in terms of ecological outcomes and in facilitating improved fire management, often are poorly documented. In the global biodiversity hotspot of southwest Western Australia, chaining and burning is a novel technique for manipulating fuels. Vegetation first is dislodged using a chain, then after a period of curing, burnt. We tested whether combining two disturbance events in this way results in different vegetation structure postfire than only burning, and whether the postfire sprouting capacity of community-dominant Eucalyptus spp. is compromised. Both chained and burnt and only burnt treatments had much less leaf litter and vegetation > 25 cm high than long-unburnt vegetation, indicating a fire management benefit of fuel modification. Chained and burnt strips had a threefold reduction in standing dead vegetation compared to only burnt samples. The stem number of Eucalyptus spp. was reduced by 20% in chained and burnt strips compared to only burnt vegetation, indicating that consecutive disturbances reduce resilience and might render sprouters vulnerable to subsequent disturbances. Balancing the fire management benefits of chaining and burning with the ecological consequences is a significant challenge facing land managers in this fire-prone landscape.     

An anatomical and clinical review of cricopharyngeal achalasia in the dog

Cricopharyngeal achalasia is a rare cause of dysphagia in the dog. However it must be differentiated from other causes of dysphagia as it is treatable with surgery. It is a disruption of the cricopharyngeal phase of the oropharyngeal phase of deglutition. There appears to be an incoordination in the swallowing process between the relaxation of the rostral, middle pharyngeal muscles and the caudal pharyngeal muscles. It is seen as a primary condition in young animals presenting soon after weaning onto solid food. The dogs appear clinically healthy unless there is secondary aspiration pneumonia or emaciation. These dogs may present as respiratory emergencies and require intensive support and treatment prior to corrective surgery. The diagnosis is made on videofluoroscopy. The condition carries a good prognosis for cure with surgical myectomy of the cricopharyngeal muscle and the thyropharyngeal muscle, which make up the upper oesophageal sphincter. Temporary relief prior to surgery can be achieved by injection of the cricopharyngeal muscle with botulism toxin. Surgical treatment for dysphagia secondary to an underlying neurological, neuromuscular or pharyngeal weakness carries a guarded prognosis and will make aspiration pneumonia worse.     

Effect of Estradiol-17β on protein synthesis and degradation rates in fused bovine satellite cell cultures

Although androgenic and estrogenic steroids are widely used to enhance muscle growth and increase feed efficiency in feedlot cattle, their mechanism of action is not well understood. Further, in vivo studies indicate that estradiol (E2) affects muscle protein synthesis and/or degradation, but in vitro results are inconsistent. We have examined the effects of E2 treatment on protein synthesis and degradation rates in fused bovine satellite cell (BSC) cultures. Additionally, to learn more about the mechanisms involved in E2-enhanced muscle growth, we have examined the effects of compounds that interfere with binding of E2 or insulin-like growth factor (IGF)-1 to their respective receptors on E2-induced alterations in protein synthesis and degradation rates in BSC cultures. Treatment of fused BSC cultures with E2 results in a concentration-dependent increase (P < 0.05) in protein synthesis rate and a decrease (P < 0.05) in protein degradation rate. The pure estrogen antagonist ICI 182 780 suppresses (P < 0.05) E2-induced alterations in protein synthesis and degradation in fused BSC cultures. The G-protein coupled receptor (GPR)-30 agonist G1 does not affect either synthesis or degradation rate, which establishes that GPR30 does not play a role in E2-induced alterations in protein synthesis or degradation. JB1, a competitive inhibitor of IGF-1 binding to the Type 1 insulin-like growth factor receptor (IGFR-1), suppresses (P < 0.05) E2-induced alterations in protein synthesis and degradation. In summary, our data show that E2 treatment directly alters both protein synthesis and degradation rates in fused BSC cultures via mechanisms involving both the classical estrogen receptor (ER) and IGFR-1.     

Effect of N‐Acetylcysteine Supplementation on Intracellular Glutathione,Urine Isoprostanes,Clinical Score,and Survival in Hospitalized Ill Dogs

Background

Antioxidant depletion and lipid peroxidation have been correlated with disease severity and associated with poor outcomes.

Hypothesis/Objectives

Supplementing dogs with N‐acetylcysteine (NAC) during the first 48 hours of hospitalization will increase cysteine, normalize glutathione concentrations, and decrease the degree of lipid peroxidation associated with illness.

Animals

Sixty systemically ill hospitalized client‐owned dogs and 14 healthy control dogs.

Methods

Randomized investigator‐blinded, placebo‐controlled prospective study. Dogs were randomized to treatment with NAC (n = 30) versus placebo (n = 30). Antioxidants, urine 8‐isoprostane/creatinine (IP/Cr), and clinical score were determined before and after treatment with NAC. Glutathione, cysteine, and vitamin E concentrations were quantified using high‐performance liquid chromatography. Atomic absorption spectroscopy and enzyme‐linked immunosorbent assays were used to quantify selenium and isoprostane concentrations, respectively.

Results

Ill dogs had significantly lower vitamin E concentrations (27 versus 55 μg/mL; P = .0005) as well as elevated IP/Cr ratios (872 versus 399 pg/mg; P = .0007) versus healthy dogs. NAC supplementation significantly increased plasma cysteine (8.67 versus 15.1 μM; P < .0001) while maintaining glutathione concentrations. Dogs in the placebo group experienced a statistically significant decrease in glutathione concentrations (1.49 versus 1.44 mM; P = .0463). Illness severity and survival were unchanged after short duration NAC supplementation.

Conclusions

Ill dogs experience systemic oxidative stress. Supplementation with NAC during the first 48 hours of hospitalization stabilized erythrocyte glutathione concentrations. The clinical impact of this supplementation and glutathione concentration stabilization was undetermined.     

Section A. Physiology and Behaviour

An effective live-trap was designed for Cryptomys hottentotus (Bathyergidae) arid Amblysomus hottentotus (Chrysochloridae). Factors involved in the design and adaptability of this trap for use in various field conditions and live capture of other fossorial mammals are discussed.