Effects of hypoxia on the expression of heparanase and invasiveness of SKOV3 ovarian cancer cell line

AIM: To evaluate the effects of hypoxia on the expression of heparanase and the invasiveness of SKOV3 ovarial carcinoma cell line. METHODS: SKOV3 cells were incubated at either normoxia (37 ℃, 5％CO2, 21％O2) or hypoxia (37 ℃, 5％CO2, 1％O2) condition for 12 h, 24 h and 36 h. RT-PCR and Western blotting were used to detect mRNA and the protein expressions of heparanase under different conditions. Cell invasiveness was measured by matrigel invasion assay. RESULTS: Compared to normoxia group, the heparanase mRNA expression level in hypoxia group was increased and in 12 h hypoxia group was the highest. The heparanase protein expression in hypoxia group was also significantly increased (P<0.01) and the expression of heparanase in hypoxia group was also different (P<0.05). Compared to normoxia group, the level of cell invasion was markedly increased in 12 h, 24 h and 36 h groups (P<0.05). During 12-36 h hypoxia period, the increase in hypoxia-induced invasiveness in SKOV3 cell line showed a time-dependent manner (P<0.05). Meanwhile, there was a positive correlation between the expression of HPA and the invasiveness of SKOV3 cells (r=0.8530, P<0.05). CONCLUSION: Invasion of SKOV3 cells in hypoxia condition correlates with heparanase level. Hypoxia plays an important role in the augmentation of the heparanase expression and the invasiveness of human ovarian cancer.     

家蚕促产卵剂的再探讨

本文就促排三号、医用催产素对促进家蚕蛾产卵的效果及试用方法进行探讨。试验证明，前者可提高产出卵经约５％，但因品种而有差异。后者对临产蛾有加速速度的作用。     

木工带锯条系统固有频率声学测量

采用声学测量法测试木工带锯条系统的固有频率,对带锯条研发与检测技术具有一定的应用价值,并为各类木工机械与刀具系统的固有频率测试提供借鉴的方法.     

犬毛滴虫病的诊治1例

犬毛滴虫病是由五鞭毛滴虫引起的以幼犬粘液性出血性腹泻为特征的一种人畜共患寄生虫。笔者在临床上接诊一例犬的慢性腹泻，经病史调查、临床检查、实验室检查确诊为犬毛滴虫病，应用甲硝唑治疗5天痊愈出院     

正交试验法在龙眼和荔枝组织培养中的应用

利用正交设计试验法研究了椰乳（ＣＭ）、２，４－Ｄ、赤霉素（ＧＡ３）和蔗糖等四个因素及其组合对石硖龙眼和桂味荔枝的幼胚愈伤组织产生的影响，并讨论了正交设计试验法应用于龙眼和荔枝组织培养培养基筛选的可能性。结果表明：１）对龙眼，诱导愈伤组织的最优组合是 ＣＭ １０％、２，４－Ｄ１．０ｍｇ·１－１、ＧＡ３０ｍｇ·１－１和蔗糖３％；对荔枝，最优组合是ＣＭ０％，２，４－Ｄ１．０ｍｇ·１－１，ＧＡ３０ｍｇ·１－１和蔗糖 ５％。 ２）适当的 ２，４－Ｄ浓度（１．０ ｍｇ·１－１）可提高愈伤组织的产量；３）ＧＡ３抑制了龙眼和荔枝的愈伤组织的产生；４）不同树种的胚需要不同的糖浓度；５）适当ＣＭ浓度（１０％）可提高龙眼愈伤组织的产量，而荔枝愈伤组织的产生并不需要ＣＭ。     

柠檬桉袋蛾的生物学特性与防治

柠檬桉袋蛾是柠檬桉重要的食叶害虫。该虫１年发生２代。在该虫为害区更换树种和营造混交林可有效地控制此虫的成灾；化学防治可用２５％灭幼脲Ⅲ号０．５～１．０ｍｌ／Ｌ溶液喷雾，防治效果达９０％以上。     

棉花铃重AFLP标记的初步研究

本文以陆地棉铃重近等基因系为材料(大铃≥5.4g,小铃≤1.7g),构建大小铃两个近等基因池.利用AFLP标记技术对两基因池进行分析,64对引物组合共产生3840条稳定、清晰扩增带.五条扩增带在两基因池间呈现多态性,其中四条差异带出现在大铃基因池,编号分别为LB-1、LB-2、LB-3和LB-4;一条出现在小铃基因池,编号为SB-1.模板浓度梯度分析表明,在一定范围内,酶切模板浓度对AFLP结果影响较小.利用稍作修改的CTAB法提取的棉花DNA可用于AFLP分析.     

营养液对毛竹叶片效应的研究 Ⅱ.营养液对叶片光合性状影响

在毛竹丰产林中 ,设置 4块标准地 ,1 996年 1 1月注射毛竹营养液 ,1 997年夏在各注射和未注射营养液的标准地中取 1 992～ 1 997年生毛竹枝叶测定光合速率、光呼吸、暗呼吸、CO2 补偿点、光补偿点等光合性状。结果表明 ,施用毛竹营养液后叶质重、叶绿素含量明显提高 ,净光合速率增大 ,暗呼吸有所提高 ,光呼吸速率减小 ,CO2 补偿点和光补偿点明显下降 ,这些指标的改变有利于提高光合作用、增加有机质积累 ,有利于培养高产稳产竹林。     

Effect of fibrin on expression of interleukin-6 in rat brain vascular endothelial cells

AIM:To investigate the kinetics of interleukin-6 (IL-6) protein and mRNA expressions in the co-culture of rat brain vascular endothelial cells(VECs) with fibrin. METHODS:The IL-6 concentration were measured by rat IL-6 ELISA kit and the IL-6 mRNA was measured by real-time PCR after development an in vitro model of in situ fibrin polymerization on rat brain vascular endothelial cells. RESULTS:Fibrin induced IL-6 expression. The IL-6 antigen concentration increased significantly in 1.0 g/L media group compared to the low dose fibrin group or control media group (P<0.01). Fibrin-induced IL-6 expression in rat brain VECs as early as 2 h post-fibrin stimulation, increased significantly at 8 h, keeping at high level till 24 h (P<0.05). Collagen did not induce IL-6 expression at 24 h. Real-time PCR analysis showed that IL-6 antigen increase in fibrin treated rat brain VECs was due to fibrin induced elevation of steady state mRNA expression. CONCLUSION:Fibrin is a potent vascular endothelial cell activator. It induces IL-6 expression in time and dose dependent fashions.     

中国畜牧行业,外资侵袭下危机四伏?

改革开放以来，我国的农业特别是畜牧行业得到快速发展，WTO以后市场格局出现隐性分化，呈现出表面的市场混乱和潜在的食品安全问题，如何理清和看待当前的发展，是目前很多畜牧行业的企业所困惑的问题，也是当前形势下急于突破的瓶颈。