番茄乙烯受体基因反义表达对果实成熟的影响

 以乙烯受体基因LeETR1和LeETR2的转反义基因株系ale1和ale2为材料, 对果实成熟特性进行了研究。转基因番茄果实与对照相比, 呼吸速率下降, 但乙烯释放速率增加1～4倍, 呼吸高峰和乙烯跃变高峰出现的时间没有改变, 表明果实成熟起始时间没有改变。ale1果实成熟后期着色速度减慢, 红熟果色泽a值、番茄红素和类胡萝卜素含量都显著低于对照; 但果实多聚半乳糖醛酸酶活性上升和硬度下降速度都显著高于对照。这说明LeETR1基因与果实成熟品质特征的形成可能存在比较复杂的关系。     

饲料中呕吐毒素的危害与防治

呕吐毒素是一种常见的真菌毒素,在饲料广泛存在。本文综述了霉菌呕吐毒素的污染状况、对畜禽的危害及其毒性机理,并提出了防治措施。     

犬瘟热病毒R／20-8株核衣壳蛋白基因的克隆和表达

应用RT-PCR技术扩增出犬瘟热病毒（CDV）核衣壳（N）蛋白基因的高度保守序列，将其克隆至质粒pMD18-T中，获得了重组质粒pMD18-T-N。将N基因的目的片段克隆到表达载体pGEX-4T-1中谷胱甘肽转移酶（GST）基因的下游，并将该重组质粒转化大肠杆菌BL21株，经IPTG诱导，N基因融合蛋白获得了高效表达。SDS-PAGE电泳和Western—blot分析结果显示，表达产物的分子质量为55ku，与CDV标准阳性血清呈阳性反应。表明，大肠杆菌表达的CDVN蛋白在免疫原性上与天然N蛋白具有一定的相似性，可作为诊断用抗原。     

碳源和氮源对黑曲霉产木聚糖酶的影响

研究了碳源、氮源以及其它因子对黑曲霉(Aspergillusniger)A3菌株产木聚糖酶的影响。结果表明,当迟效碳源为玉米芯70g/l、速效碳源为葡萄糖10g/l,氮源为NaNO33g/l和(NH4)2SO43g/l,麸皮5g/l,用无氮的Mandels无机盐溶液配制,起始pH值5.5～6.0,并添加中和剂CaCO3调控发酵pH值,250ml三角瓶装液量为30ml时,黑曲霉A3液体发酵酶活力达405.6IU/ml。采用玉米芯代替半纤维素作为主要碳源,可以大大降低生产成本,减少环境污染,有利于工业化生产。     

益生菌固态生物发酵的研究

益生菌作为生物兽药已应用到了实际生产中,它具有良好的防治消化道疾病和调节体内微生态平衡、提高机体免疫力、降低和中和毒素、促进康复等功能。并具有无毒、无害、无污染、无残留的优势。但常规的生物制品方法使其代谢产物中的氨基酸、维生素、抗菌肽、有机酸、各种酶系等没     

29个微卫星标记与淮南麻黄鸡体重的相关性分析

利用29个微卫星标记的多态性分析淮南麻黄鸡12周龄体重与微卫星标记的相关。结果表明:位于3号染色体和10号染色体上的2个微卫星标记MCW0222、ADL0122与淮南麻黄鸡12周龄体重显著相关。     

用间接血凝试验检测牛肺疫血清抗体的研究

本文试验结果表明:1、不同个体绵羊红细胞对间接血凝的敏感性有很大影响,在实际工作中应给予充分注意。2、间接血凝诊断液置4℃冰箱保存30d 效价无明显改变。3、用间接血凝方法检测85份血清样本,检出率为8/85,高于补体结合反应(2/85),重复性试验结果稳定。     

蔬菜冷冻贮藏营养成分变化的研究

对蔬菜冷冻贮藏营养成分变化的研究表明，随着冷冻贮藏时间的延长和温度的升高，蔬菜中４种维生素的损失率均不同程度地增加，损失规律基本一致。除杀菁瓠瓜外，低温处理使杀菁豇豆和未杀菁马齿苋中氨基酸态氮含量先升后降。低温处理有利于控制蔬菜中硝态氮的含量，但对可溶性总糖含量的影响却较小。     

Differential Colonization of Tomato Roots by Nonpathogenic and Pathogenic Fusarium oxysporum Strains May Influence Fusarium Wilt Control

ABSTRACT Histochemical staining, beta-glucuronidase (GUS) activity, or placing roots on agar were methods used to characterize interactions between the pathogenic fungus, Fusarium oxysporum f. sp. lycopersici, and the nonpathogenic biocontrol F. oxysporum strain 70T01 with respect to colonization behaviors, interaction sites, and population densities on tomato roots. Mycelia of strain 70T01, a genetic transformant expressing stable GUS activity, hygromycin B resistance, and effective disease control, were localized in epidermal and cortex cell layers of tomato roots in a discontinuous and uneven pattern. In contrast, mycelia of F. oxysporum f. sp. lycopersici were found in the vascular bundles. Thus, direct interactions between the two fungi likely happen in the root surface cell layers. Colonization density of strain 70T01 was related to the inoculation density but decreased with distance from the inoculation site. Host defense reactions, including increased cell wall thickness or papilla deposits, were adjacent to 70T01 hyphae. Experiments done in soil showed that strain 70T01 densities in roots were highest at inoculation zones and barely detectable for root segments more than 2 cm away from the inoculation sites. F. oxysporum f. sp. lycopersici densities were lowest at 70T01 inoculation zones and highest (>10 times) where strain 70T01 was not directly applied. Newly elongating roots where strain 70T01 did not reach were available for infection by the pathogen. The higher strain 70T01 density was always found when the plants were simultaneously infected by F. oxysporum f. sp. lycopersici, suggesting that F. oxysporum f. sp. lycopersici has as much influence in predisposing the plant to colonization by strain 70T01 as strain 70T01 has on providing disease protection against the pathogen.