鸡传染性支气管炎病毒单克隆抗体杂交瘤细胞系的建立

以提纯的IBV-M病毒抗原免疫BALB/C小鼠,末次免疫后三天取脾细胞与SP_2/O细胞在50％PEG2000作用下融合,三次融合两次获得成功。经抗体检测、筛选和克隆化培养,最后获得三株分泌特异性单克隆抗体的杂交瘤细胞系。三株杂交癌细胞经体外连续传代培养2个多月,以及液氮冻存4个月后复苏培养,仍能稳定地分泌抗体。     

Quick-cooking beans (Phaseolus vulgaris L.): II. Phytates,oligosaccharides, and antienzymes

Effects of the quick-cooking processes on phytate and oligosaccharide levels, and on trypsin and chymotrypsin inhibitors, were investigated in three bean varieties (Phaseolus vulgaris L.: Great Northern, red kidney, and pinto). Beans soaked in distilled water had lower levels of phytate-P than those soaked in a mixed salt solution. Leaching losses of oligosaccharides were nearly the same in different soaking treatments for all the beans except kidney beans. Residual trypsin inhibitor activities (TIA) in cooked quick-cooking beans were about 10% compared with about 20% for chymotrypsin inhibitor activities (CTIA) in the same bean products. -Irradiation was more effective in reducing TIA than CTIA and paralleled destruction by moist heat.Utah Agricultural Experiment Station Journal article no. 2480.     

Role of thyroid hormones in tilapia larvae (Oreochromis mossambicus): I. Effects of the hormones and an antithyroid drug on yolk absorption,growth and development

Treatment of one-day old yolksac larvae of tilapia (Oreochromis mossambicus) by immersion in 0.05 ppm T₄ or 0.01 ppm T₃ significantly accelerated the differentiation and growth of all the fins, particularly pectoral and tail fins. Both the treatments also significantly accelerated yolk absorption and transition to free-swimming activity in the larvae. The treatments also significantly accelerated the growth of the larvae, with T₃ at 0.01 ppm having a greater effect than T₄ at 0.05 ppm. The yolk conversion efficiency was found not to be significantly affected by the hormone treatments but the treated larvae exhibited an increased heart beat, suggesting metabolic stimulation by the hormones.On the other hand, yolk absorption and free-swimming activity were significantly delayed in tilapia larvae immersed in 25 ppm solution of an antithyroid drug, phenylthiocarbamide (PTC). PTC also retarded the growth of the larvae. T₄ (0.05 and 0.10 ppm) or T₃ (0.01 and 0.02 ppm) therapy removed the PTC-inhibition,albeit not completely, suggesting that thyroid hormones are involved in the larval growth and development of tilapia.     

Multiple Interval Mapping for Whole Cocoon Weight and Related Economically Important Traits QTL in Silkworm （Bombyx mort）

A backcrossed population （BC1） derived from a cross between C100 and Dazao was obtained. The quantitative trait loci （QTLs） of the economically important traits for whole cocoon weight, cocoon shell weight, ratio of cocoon shell and weight of pupae, etc., were analyzed for the first time using the multiple interval mapping software WinQTLCart2.0. In total 40 QTLs were detected and contributed to 21 groups based on the constructed linkage map. According to the mapping results, 2, 2, 3, and 2 major QTLs explained over 20% of total phenotypic variations, whereas four QTLs, namely qCW-19, qSW-2, qCSR-4, and qPW-23, explained more than 30% of total phenotypic variations for whole cocoon weight, cocoon shell weight, ratio of cocoon shell and weight of pupae, respectively. Correlated traits QTLs often share the same location. Furthermore, most of the detected QTLs were closed to one-side marker. By using the very closed markers, positive QTLs can be aggregated, which can form a basis for molecular marker-assisted selection and breeding.     

Die Schwarzamsel (Turdus merula), deren Schaden und Versuche zur Abwehr

Ohne Zusammenfassung     

不同种源火炬松和湿地松木材基本密度和管胞长度的变异

对1984年种植在广西和浙江的22个种源的火炬松及在浙江的8个种源的湿地松的木材密度和管胞长度进行了测定和分析。结果表明,两个树种的木材密度和管胞长度在种源间和种源内单株间均表现出显著差异,但株间差异较种源间差异显著得多。不同地区火炬松的差异较之同一地区不同种源间的差异更为显著。种源、地区的交互作用对火炬松的木材密度和管胞长度都表现出显著影响,种源和单株的交互作用对两个树种的木材密度也有显著影响。两个树种的木材材性与年轮宽度之间没有显著的相关关系。与马尾松和早期引种的火炬松、湿地松比较表明,用新引进的材料可选育出木材材质更为优良的品种。