Influence of Bordetella avium infection on association of Escherichia coli with turkey trachea

Four-week-old Bordetella avium-infected and B avium-free turkeys were inoculated intratracheally with a suspension of fimbriated or nonfimbriated Escherichia coli. Numbers of E coli associated with tracheal sections were determined at postinoculation hour (PIH) 1 or 6. Significantly (P less than 0.05) greater numbers of E coli were isolated from the tracheas of B avium-infected turkeys compared with numbers in B avium-free turkeys. In B avium-free turkeys, tracheal associated E coli were 90% less at PIH 6 compared with that at PIH 1. However, in B avium-infected turkeys, numbers of E coli were not affected by postinoculation time. Seemingly, B avium-infected turkeys had reduced capacity to clear E coli from the trachea.     

A diiron protein autogenerates a valine-phenylalanine cross-link

All known internal covalent cross-links in proteins involve functionalized groups having oxygen, nitrogen, or sulfur atoms present to facilitate their formation. Here, we report a carbon-carbon cross-link between two unfunctionalized side chains. This valine-phenyalanine cross-link, produced in an oxygen-dependent reaction, is generated by its own carboxylate-bridged diiron center and serves to stabilize the metallocenter. This finding opens the door to new types of posttranslational modifications, and it demonstrates new catalytic potential of diiron centers.     

AGEING OF HYDROLYSED IRON (III) SOLUTIONS1

Three 0.01m Fe(ClO₄)₃ Solutions of R= 0, 1, and 2, respectively, were studied over a period of 8 months, where R is referred to the HCO₃^?/Fe³⁺ mole ratio in preparation. The R= 0 solution was initially light yellow and clear but rapidly changed to a dense cloudy Suspension after a 9-day induction period. Hydrolysed species rapidly grew from <10 nm to >650 nm. Particles of intermediate size were not observed during the entire period of study. Settleable FeOOH precipi-tate was first observed in 5 weeks with 23 per cent of its iron noted as precipitate at the end of 5 months. The R= 1 and R= 2 Solutions were reddish-brown of low turbidity. Rapid hydrolysis and polymerization were observed immediately after preparation. The hydrolysed species gradually grew in size during ageing and no settleable FeOOH precipitate was observed for at least two years. A hypo-thesis based on Lamb and Jacques's model of Fe³⁺ hydrolysis is proposed to interpret the reactions taking place in these three Solutions. In the R= 0 solution, the initial number of nuclei for condensation is low, whereas the poten-tial supply of Fe(OH)₃ is abundant. In the R= 1 and R= 2 Solutions the number of nuclei initially formed is high, whereas a limited amount of mono-meric Fe(OH)₃ is available for condensation. The initial number of nuclei relative to the concentration of monomeric species is the key factor that governs the appearance and properties of these Solutions.     

Anaesthesia with ketamine/medetomidine in the rabbit: influence of route of administration and the effect of combination with butorphanol

Objective To compare the characteristics of anaesthesia induced with ketamine/medetomidine administered by the subcutaneous and intramuscular routes and to assess the effects of the addition of butorphanol to this combination. Study design Prospective randomised study. Animals Six female New Zealand White rabbits. Methods Rabbits were given one of four combinations of ketamine and medetomidine (K/M) either subcutaneously (SC) or intramuscularly (IM) on four successive occasions with a 7‐day interval between treatments. The dose combinations were; 15/0.25 mg kg^?1 SC; 15/0.25 mg kg^?1 IM; 15/0.5 mg kg^?1 SC, and 15/0.25 mg kg^?1 together with 0.4 mg kg^?1 butorphanol (K/M/B) SC. The effects of anaesthesia on arterial blood gas values and cardiovascular variables were recorded at predetermined time points. Toe and ear pinch reflexes were judged to determine the duration of surgical anaesthesia. Loss of the righting reflex was used to measure the duration of sleep time. Analyses used repeated measures analysis of variance. Results All groups lost the righting reflex and ear pinch response. Three animals in the groups that received K/M alone lost their toe pinch reflex, whereas four lost this reflex when given K/M/B. Time of onset of loss of the righting, toe and ear pinch reflexes did not differ significantly among the groups. The higher dose combination of medetomidine with ketamine and the combination of K/M/B produced a greater duration of loss of the ear pinch response than the lower dose of K/M administered by either route. No significant differences were found among the groups in the duration of loss of the toe pinch reflex. All animals developed a moderate bradycardia (mean heart rate <166 beats minute^?1) and moderate hypoxaemia (mean P_aO₂ < 6.0 kPa). Animals given butorphanol showed the greatest reduction in respiratory rate (31 ± 13 breaths minute^?1, p < 0.05) but this was not reflected in any significant differences in arterial PCO₂, PO₂ or pH among the groups. Conclusions Administration of K/M by the SC route produced equivalent effects in comparison to intramuscular administration. The addition of butorphanol increased the duration of anaesthesia, but produced a slight increase in the degree of respiratory depression. All dose rates resulted in hypoxaemia so oxygen should be administered when these combinations are used in rabbits. Clinical relevance Subcutaneous administration is both technically simpler and may cause less discomfort to the animal than IM injection, and so is preferred. The combination of K/M with butorphanol has relatively minor effects on the depth and duration of anaesthesia, so offers little advantage to the use of K/M alone.     

论高校图书馆流通服务中的＂关键时刻＂管理

分析了高校图书馆流通服务和赢利性企业的共性,论述了高校图书馆流通服务管理应用“关键时刻”管理理论的可行性和必要性。提出了通过对高校图书馆流通服务中的一系列“关键时刻”的强化管理．提高流通服务质量,最大限度地满足读者需求的策略。     

Post-vaccinal distemper encephalitis in two Border Collie cross littermates

Abstract

CASE HISTORY: One 4.5-month-old male Border Collie cross presented with aggression and seizures in October 2006. A 16-month-old, female, spayed Border Collie cross presented with hypersalivation and a dropped jaw and rapidly became stuporous in September 2007. The dogs were littermates and developed acute neurological signs 5 and 27 days, respectively, after vaccination with different modified live vaccines containing canine distemper virus.

HISTOPATHOLOGICAL FINDINGS: Sections of brain in both dogs showed evidence of encephalitis mainly centred on the grey matter of brainstem nuclei, where there was extensive and intense parenchymal and perivascular infiltration of histiocytes and lymphocytes. Intra-nuclear and intra-cytoplasmic inclusions typical of distemper were plentiful and there was abundant labelling for canine distemper virus using immunohistochemistry.

DIAGNOSIS: Post-vaccinal canine distemper.

CLINCIAL RELEVANCE: Post-vaccinal canine distemper has mainly been attributed to virulent vaccine virus, but it may also occur in dogs whose immunologic nature makes them susceptible to disease induced by a modified-live vaccine virus that is safe and protective for most dogs.     

Robust reconstitution of active cell-cycle control complexes from co-expressed proteins in bacteria

ABSTRACT: BACKGROUND: Cell proliferation is an important determinant of plant growth and development. In addition,modulation of cell-division rate is an important mechanism of plant plasticity and is key inadapting of plants to environmental conditions. One of the greatest challenges inunderstanding the cell cycle of flowering plants is the large families of CDKs and cyclins thathave the potential to form many different complexes. However, it is largely unclear whichcomplexes are active. In addition, there are many CDK- and cyclin-related proteins whosebiological role is still unclear, i.e. whether they have indeed enzymatic activity. Thus, abiochemical characterization of these proteins is of key importance for the understanding oftheir function. RESULTS: Here we present a straightforward system to systematically express and purify active CDKcyclincomplexes from E. coli extracts. Our method relies on the concomitant production of aCDK activating kinase, which catalyzes the T-loop phosphorylation necessary for kinaseactivity. Taking the examples of the G1-phase cyclin CYCLIN D3;1 (CYCD3;1), the mitoticcyclin CYCLIN B1;2 (CYCB1;2) and the atypical meiotic cyclin SOLO DANCERS (SDS) inconjunction with A-, B1- and B2-type CDKs, we show that different CDKs can interact withvarious cyclins in vitro but only a few specific complexes have high levels of kinase activity. CONCLUSIONS: Our work shows that both the cyclin as well as the CDK partner contribute to substratespecificity in plants. These findings refine the interaction networks in cell-cycle control andpinpoint to particular complexes for modulating cell proliferation activity in breeding.