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951.
Background, Aims, and Scope  Hydroxy interlayered vermiculite (HIV) and vermiculite are commonly referred to as 1.4 nm minerals. In the subtropical soils of central China, the concentration of vermiculites decreased while that of HIVs increased gradually from north to south as the intensity of soil formation or eluviation increases in the same direction. The cutans in these soils closely interact with air, roots, microbes, water and dissolved ions in soils. Cutans may therefore be expected to exert an important influence on the formation of 1.4 nm minerals relative to the matrix soils. However, little is known about the transformation of 1.4 nm minerals in Alfisols in central China. Here, we investigate the compositional differences of 1.4 nm minerals in cutans and matrix soils, and the probable transformation of vermiculite to HIV or vice versa when sodium citrate and sodium acetate are added to matrix Alfisols. Methods  Cutans and matrix soils were separated from three soils in the northern subtropical zone in China. The samples were analyzed for Fe, Mn, exchangeable cations, organic matter(O.M.), pH, and clay minerals. To 10 mL of matrix soil, suspensions containing about 250 mg (oven-dry weight) of clay was added with 5 mL of 0.4 mol/dm3 or 2 mol/dm3 of sodium citrate or sodium acetate solution and 5 mL of 0.2 mol/dm3 mixed solutions of CaCl2, Mg(NO3)2 and KCl. After its pH was adjusted to 6.0, the mixture was ‘incubated’ for 120 or 210 days (more than one season or half a year) during which period it was shaken for 1 hour every day. The clay mineral composition of the samples was determined after incubation. Results  Both vermiculites and HIVs were present in matrix soils, but only vermiculties were detected in cutans. The addition of organic ligands (citrate and acetate) promoted the transformation of HIV to vermiculite. This transformation was obvious for the matrix soils that had been incubated with 0.5 mol/dm3 sodium citrate for 210 days while sodium acetate was less effective in this regard. The promoting effect of organic ligands is dependent on type and concentration as well as incubation time. This would suggest the reverse transformation occurred in the formation of cutans compared with a vermiculite-to-HIV transformation in the subtropical soils of central China from north to south. Discussion  The position and environment of cutans in the B horizon together with the pH, organic matter and exchangeable base status in cutans seem conducive to the co-existence of vermiculite and HIV in the soils, but only vermiculite is found in cutans. The transformation of HIV to vermiculite in incubation experiments could be divided into two steps: 1) Cheluviation of organic matter to the interlayer hydroxy-aluminums from HIVs. 2) Rebasification of hydrated cations into the interlayers of vermiculites. Conclusions  The clay minerals in cutans can interact with organic ligands and nutrient elements excreted by roots. Under conditions of frequent wetting and drying and high pH, and when the concentrations of exchangeable bases, iron-manganese oxides, clays, and organic matter are high, the exchangeable cations can be incorporated into the interlayers of HIV, thereby promoting the partial transformation of HIV to vermiculite in rhizosphere soils. Recommendations and Perspectives  Cutan is at the interface of material and energy exchange involved in physical, chemical and biochemical reactions in the rhizosphere. These factors strongly affect the compositions of cutans. HIVs in (upper or adjacent) matrix soils may transform to vermiculites during cutan formation in these special soil environments. ESS-Submission Editor: Jizheng (Jim) He (jzhe@rcees.ac.cn)  相似文献   
952.
Genetic diversity of seven Rhus L. species was assessed using random amplified polymorphic DNA (RAPDs) markers. Initially, 90 primers were screened, of which 25 produced reproducible amplification products. These primers generated a total of 296 bands, with an average of 11.8 bands per primer. Out of 296 bands scored, 236 (80%) were polymorphic and 62 (20%) were monomorphic. Primers OPC-05 and OPD-05 generated 100% polymorphic bands. The resolving power of primers ranged from 9.4 to 26.8. Similarity matrix values ranged from 0.45 to 0.63. The dendrogram generated using Unweighted Pair Group Method using Arithmetic Averages (UPGMA) grouped all the species of Rhus in one major group with two sister groups, whilst R. pyroides Burch. and R. dentata Thunb. were outliers. R. gerrardii (Harv. ex Engl.) Diels, R. glauca Thunb. and R. pentheri Zahlbr. constituted one sister group, while R. natalensis Bernh. ex C. Krauss and R. gueinzii Sond. were included in the other. The degree of genetic diversity observed between seven species of Rhus with RAPD markers suggest that this approach could be used for studying the phylogeny of the genus.  相似文献   
953.
Temporal dynamics of microbial biomass and respiration of soil and their responses to topography, burning, N fertilization, and their interactions were determined in a temperate steppe in northern China. Soil microbial indices showed strong temporal variability over the growing season. Soil microbial biomass C (MBC) and N (MBN) were 14.8 and 11.5% greater in the lower than upper slope, respectively. However, the percentage of organic C present as MBC and the percentage of total N present as MBN were 16.9 and 26.2% higher in the upper than lower slope, respectively. Neither microbial respiration (MR) nor metabolic quotient (qCO2) was affected by topography. Both MBC and MBN were increased by burning, on average, by 29.8 and 14.2% over the growing season, and MR and qCO2 tended to reduce depending on the sampling date, especially in August. Burning stimulated the percentage of organic C present as MBC and the percentage of total N present as MBN in the upper slope, but did not change these two parameters in the lower slope. No effects of N fertilization on soil microbial indices were observed in the first growing season after the treatment. Further research is needed to study the long-term relationships between changes in soil microbial diversity and activity and plant community in response to burning and N fertilization.  相似文献   
954.
955.
Modeling infectious diseases data is a relatively young research area in which clustering and stratification are key features. It is not unlikely for these data to have missing values. If values are missing completely at random, the analysis on the complete cases is valid. However, in practice this assumption is usually not fulfilled. This article shows the effect of ignoring missing data in modeling the force of infection of the bovine herpesvirus-1 in Belgian cattle and proposes the use of weighted generalized estimating equations with constrained fractional polynomials as a flexible modeling tool.  相似文献   
956.
The cereal crops (barley -Hordeum vulgare L., maize -Zea mays L., wheat -Triticum vulgare L.) were grown in a greenhouse using a sandy soil type treated with various doses of cadmium carbonate (salt), copper carbonate (malachite), lead carbonate (cerussite), and zinc carbonate (smithsonite), added jointly. The following levels of these metals were used: Cd ? 5, 10, 50μg g?1 soil; Cu and Pb - 50,100, 500 μg g?1 soil; Zn-150, 300, 1500 μg g-1 soil. Sequential extraction was adopted to partition the metals into five operationally-defined fractions: exchangeable, carbonate, Fe-Mn oxides, organic, and residual. The residual was the most abundant fraction in the untreated (control) soil for all the metals studied (50 to 60% of the total metal content). The concentrations of exchangeable Cd, Cu, Pb, and Zn were relatively low in untreated soil but increased (over the three year period) in treated soils for Cd, Zn, and Cu, whereas only small changes were observed for Ph. This experiment showed a significant increase in Cd, Zn, and Cu in tissue of plants grown on the treated soil, but a non-significant change in plant tissue with respect to Pb concentration.  相似文献   
957.
958.
A fluorescence polarization immunoassay (FPIA) based on a monoclonal antibody for the detection of parathion-methyl (PM) was developed and optimized. Fluorescein-labeled PM derivatives (tracers) with different structures were synthesized and purified by thin-layer chromatography. The influence of immunogen and tracer structures on the assay characteristics was investigated. PM concentration determinable by the FPIA ranged from 25 to 10000 ppb. The detection limit was 15 ppb. Methanol extracts of vegetable, fruit, and soil samples were diluted 1/10 for the analysis. Recovery in spiked samples averaged between 85 and 110%. The method developed is characterized by high specificity and reproducibility (CV ranged from 1.5 to 9.1% for interassay and from 1.8 to 14.1% for intra-assay). The FPIA method can be applied to the screening of food and environmental samples for PM residues without complicated cleanup.  相似文献   
959.
In spatial predictions, researchers usually treat the estimated theoretical variogram parameters as known without error and ignore the variability of the parameter estimators. Although the prediction is still unbiased, the prediction error is usually underestimated. Therefore, the coverage probability of the prediction interval usually is lower than the nominal probability. A simulation study is performed to show how the coverage probability for prediction relates to the true range and sill of an exponential variogram. This article proposes two parametric bootstrap methods to incorporate the variability of the corresponding parameter estimators. A simulation study is performed to evaluate the coverage probability of these proposed methods. Finally, we apply the parametric bootstrap methods to a real dataset and compare the results with those from naive (i.e., treating estimated parameters as known) and Bayesian methods.  相似文献   
960.
Nitrogen fixation in biological soil crusts from southeast Utah,USA   总被引:20,自引:0,他引:20  
Biological soil crusts can be the dominant source of N for arid land ecosystems. We measured potential N fixation rates biweekly for 2 years, using three types of soil crusts: (1) crusts whose directly counted cells were >98% Microcoleus vaginatus (light crusts); (2) crusts dominated by M. vaginatus, but with 20% or more of the directly counted cells represented by Nostoc commune and Scytonema myochrous (dark crusts); and (3) the soil lichen Collema sp. At all observation times, Collema had higher nitrogenase activity (NA) than dark crusts, which had higher NA than light crusts, indicating that species composition is critical when estimating N inputs. In addition, all three types of crusts generally responded in a similar fashion to climate conditions. Without precipitation within a week of collection, no NA was recorded, regardless of other conditions being favorable. Low (<1°C) and high (>26°C) temperatures precluded NA, even if soils were moist. If rain or snow melt had occurred 3 or less days before collection, NA levels were highly correlated with daily average temperatures of the previous 3 days (r2=0.93 for Collema crusts; r2=0.86 for dark crusts and r2=0.83 for light crusts) for temperatures between 1°C and 26°C. If a precipitation event followed a long dry period, NA levels were lower than if collection followed a time when soils were wet for extended periods (e.g., winter). Using a combination of data from a recording weather datalogger, time-domain reflectometry, manual dry-down curves, and N fixation rates at different temperatures, annual N input from the different crust types was estimated. Annual N input from dark crusts found at relatively undisturbed sites was estimated at 9 kg ha-1 year-1. With 20% cover of the N-fixing soil lichen Collema, inputs are estimated at 13 kg ha-1 year-1. N input from light crusts, generally indicating soil surface disturbance, was estimated at 1.4 kg ha-1 year-1. The rates in light crusts are expected to be highly variable, as disturbance history will determine cyanobacterial biomass and therefore N fixation rates.  相似文献   
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