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71.
Anja Bergstermann Roland Bol Keith Goulding David Scholefield Reinhard Well 《Soil biology & biochemistry》2011,43(2):240-250
The present study determined the influence of initial moisture conditions on the production and consumption of nitrous oxide (N2O) during denitrification and on the isotopic fingerprint of soil-emitted N2O. Sieved arable soil was pre-incubated at two different moisture contents: pre-wet at 75% and pre-dry at 20% water-filled pore space. After wetting to 90% water-filled pore space the soils were amended with glucose (400 kg C ha−1) and KNO3 (80 kg N ha−1) and incubated for 10 days under a He/O2-atmosphere. Antecedent moisture conditions affected denitrification. N2 + N2O fluxes and the N2O-to-N2 ratio were higher in soils which were pre-incubated under dry conditions, probably because mobilization of organic C during the pre-treatment enhanced denitrification. Gaseous N fluxes showed similar time patterns of production and reduction of N2O in both treatments, where N2O fluxes were initially increasing and maximised 3-4 days after fertilizer application, and N2 fluxes were delayed by 1-2 days. Time courses of δ15Nbulk-N2O and δ18O-N2O exhibited in both treatments increasing trends until maximum N2 fluxes occurred, reflecting isotope fractionation during intense NO3− reduction. Later this trend slowed down in the pre-dry treatment, while δ18O-N2O was constant and δ15Nbulk-N2O decreased in the pre-wet treatment. We explain these time patterns by non-homogenous distribution of NO3− and denitrification activity, resulting from application of NO3− and glucose to the surface of the soil. We assume that several process zones were thus created, which affected differently the isotopic signature of N2O and the N2O and N2 fluxes during the different stages of the process. We modelled the δ15Nbulk-N2O using process rates and associated fractionation factors for the pre-treated soils, which confirmed our hypothesis. The site preference (SP) initially decreased while N2O reduction was absent, which we could not explain by the N-flux pattern. During the subsequent increase in N2 flux, SP and δ18O-N2O increased concurrently, confirming that this isotope pattern is indicative for N2O reduction to N2. The possible effect of the antecedent moisture conditions of the soil on N2O emissions was shown to be important. 相似文献
72.
Kipar A Aleksandersen M Benazzi C Hodge T Sukura A Wyers M;Education Committee of the ECVP/ESVP 《Journal of veterinary medical education》2007,34(4):485-491
This article describes the ECVP/ESVP Summer School in Veterinary Pathology, a new annual two-week European training facility established by the European College of Veterinary Pathologists (ECVP) in collaboration with the European Society of Veterinary Pathology (ESVP). The aim of the Summer Schools is to provide Europe-wide, harmonized, top-standard theoretical and practical post-graduate training for veterinarians specializing in veterinary pathology. In particular, it aims to support trainees in veterinary pathology in their individual preparation for the ECVP certifying examination. Ultimately, it aims to provide young pathologists with the skills and knowledge necessary to participate in international, high-quality research and the tools for applying international standards to their own research and for independent study for the ECVP certifying examination, even if they do not work in comparable academic environments and do not have the same level of local support and training. The ECVP/ESVP Summer Schools take place in European countries, with local organization from a university department of veterinary pathology. Each event comprises modules provided by internationally recognized specialists in their specific fields of expertise on different organ systems, diseases of specific species, specific techniques, and specific topics relevant to pathology, forming a cycle of four events to cover all major topics. Every two years a mock exam is organized as a tool to monitor individual progress in preparing for the ECVP certifying examination. 相似文献
73.
Engelhardt A Stock KF Hamann H Brahm R Grussendorf H Rosenhagen CU Distl O 《Berliner und Münchener tier?rztliche Wochenschrift》2007,120(11-12):490-498
Genetic parameters were estimated for prevalences of primary cataract (CAT), persistent pupillary membrane (PPM) and distichiasis (DIST) in 615 single-colored and 617 multi-colored English Cocker Spaniels (ECS) bred in the German kennel club for Spaniels (Jagdspaniel-Klub e.V.). CAT or CAT diagnosed in dogs up to three and a half years of age (early-onset cataract, CAT-early) and CAT diagnosed in dogs over three and a half years of age (late-onset cataract, CAT-late), PPM and DIST were included as binary traits in multivariate genetic analyses. Heritabilities on the underlying liability scale were 0.15 for CAT, 0.34 for CAT-early, 0.13 for CAT-late, 0.46 for PPM, and 0.62 for DIST in single-colored ECS and 0.06 for CAT, 0.13 for CAT-early, 0.14 for CAT-late, 0.10 for PPM, and 0.61 for DIST in multi-colored ECS. There were indications for a different genetic basis of CAT-early and CAT-late in single-colored ECS as genetic correlations were close to zero. In multi-colored ECS, a similar tendency for CAT-early and CAT-late could be observed. 相似文献
74.
Rocksin A Gerlach GF Schwarz S 《Berliner und Münchener tier?rztliche Wochenschrift》2007,120(1-2):42-49
The determination of quantitative data (minimal inhibitory concentrations [MIC values]) using the broth microdilution method is considered the method of choice for in-vitro susceptibility testing of bacterial pathogens. For the inoculation of microtiter plates used in the broth microdilution method, an inoculation density of 5x10(5) CFU/ml has to be achieved according to the CLSI standard. In this study, it was shown that the requested CFU/ml corresponded to an optical density of 0.00027 of a bacterial suspension determined in a spectrophotometer at 625 nm (OD625).The comparison of susceptibility data of clinical isolates determined by agar disk diffusion and, in parallel, by broth microdilution confirmed the comparability of both methods. For most antibiotics the frequency of "very major errors" was below 10%. However, a higher frequency of errors was observed for combinations of antimicrobial agents and bacteria for which no valid breakpoints are available. The comparative testing of cephalothin showed a frequency of"very major errors" of 20.3% for staphylococci and 18.4% for Enterobacteriaceae; for apramycin the frequency of "major errors"was found to be 46.0% for Enterobacteriaceae. The reference strains as recommended in the CLSI Standard M31-S1 should be used for quality assurance. 相似文献
75.
76.
Hühnlein Anja Drechsler Nadine Steinbach Peter Thieme Thomas Schubert Jörg 《植物病害和植物保护杂志》2013,120(2):57-69
Journal of Plant Diseases and Protection - Potato virus Y (PVY) is becoming increasingly important in potato growing regions worldwide. The main reason for this is an increase in the incidence of... 相似文献
77.
Anika Hudewenz Alexandra-Maria Klein Christoph Scherber Lea Stanke Teja Tscharntke Anja Vogel Alexandra Weigelt Wolfgang W. Weisser Anne Ebeling 《Biological conservation》2012,150(1):42-52
Agricultural intensification is a major driver of global environmental change. Disentangling the relative impact of losses in plant species richness and intensified management on higher trophic level organisms is important for conservation recommendations.We established different management regimes along an experimental gradient of plant species richness within “The Jena-Experiment” in Germany and quantified herbivory as well as grasshoppers and pollinators. Herbivory, grasshopper density and species richness and frequency of flower visiting pollinators were recorded two times in each of four subplots of altogether 80 plots differing in plant species richness. Each of the four subplots was subject to four different levels of fertilizer application and mowing to simulate very low, low, high and very high land use intensity.Fertilization and mowing significantly affected plant–herbivore interactions but plant species richness had no discernible effect. Grasshoppers were most abundant at high intensity subplots (3.1 individuals per m2) and least abundant on very low intensity subplots (1.3 individuals per m2). Leaf damage caused by herbivores was highest in even the very high intensity subplots (3.7%) with four mowing events per year and high amounts of fertilizer application and lowest on subplots with a low management intensity (2.4%) comprising two mowing events per year but no fertilizer application. In contrast, pollinators benefited most from lower management intensities, with only one or two mowing events and no fertilizer addition. In addition, higher numbers of flowering plant species and increased blossom cover was associated with enhanced pollinator species richness and flower visitation.Our results indicate that even in grasslands with high plant species richness, mowing and fertilization are more important drivers of herbivory and flower visitation by pollinators. Management with no more than two cuts per year and without fertilizer application in our grasslands balanced the ecosystem functions of increased pollination and decreased herbivory. 相似文献
78.
Bokhof B Buyken AE Doğan C Karaboğa A Kaiser J Sonntag A Kroke A 《Public health nutrition》2012,15(4):640-647
79.
Nikita Martens Melissa Schepers Na Zhan Frank Leijten Gardi Voortman Assia Tiane Ben Rombaut Janne Poisquet Nienke van de Sande Anja Kerksiek Folkert Kuipers Johan W. Jonker Hongbing Liu Dieter Lütjohann Tim Vanmierlo Monique T. Mulder 《Marine drugs》2021,19(4)
We recently found that dietary supplementation with the seaweed Sargassum fusiforme, containing the preferential LXRβ-agonist 24(S)-saringosterol, prevented memory decline and reduced amyloid-β (Aβ) deposition in an Alzheimer’s disease (AD) mouse model without inducing hepatic steatosis. Here, we examined the effects of 24(S)-saringosterol as a food additive on cognition and neuropathology in AD mice. Six-month-old male APPswePS1ΔE9 mice and wildtype C57BL/6J littermates received 24(S)-saringosterol (0.5 mg/25 g body weight/day) (APPswePS1ΔE9 n = 20; C57BL/6J n = 19) or vehicle (APPswePS1ΔE9 n = 17; C57BL/6J n = 19) for 10 weeks. Cognition was assessed using object recognition and object location tasks. Sterols were analyzed by gas chromatography/mass spectrometry, Aβ and inflammatory markers by immunohistochemistry, and gene expression by quantitative real-time PCR. Hepatic lipids were quantified after Oil-Red-O staining. Administration of 24(S)-saringosterol prevented cognitive decline in APPswePS1ΔE9 mice without affecting the Aβ plaque load. Moreover, 24(S)-saringosterol prevented the increase in the inflammatory marker Iba1 in the cortex of APPswePS1ΔE9 mice (p < 0.001). Furthermore, 24(S)-saringosterol did not affect the expression of lipid metabolism-related LXR-response genes in the hippocampus nor the hepatic neutral lipid content. Thus, administration of 24(S)-saringosterol prevented cognitive decline in APPswePS1ΔE9 mice independent of effects on Aβ load and without adverse effects on liver fat content. The anti-inflammatory effects of 24(S)-saringosterol may contribute to the prevention of cognitive decline. 相似文献
80.
Peter Annighöfer Aitor Ameztegui Christian Ammer Philippe Balandier Norbert Bartsch Andreas Bolte Lluís Coll Catherine Collet Jörg Ewald Nico Frischbier Tsegay Gebereyesus Josephine Haase Tobias Hamm Bastian Hirschfelder Franka Huth Gerald Kändler Anja Kahl Heike Kawaletz Christian Kuehne André Lacointe Na Lin Magnus Löf Philippe Malagoli André Marquier Sandra Müller Susanne Promberger Damien Provendier Heinz Röhle Jate Sathornkich Peter Schall Michael Scherer-Lorenzen Jens Schröder Carolin Seele Johannes Weidig Christian Wirth Heino Wolf Jörg Wollmerstädt Martina Mund 《European Journal of Forest Research》2016,135(2):313-329