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961.
Hiromichi Mitamura Keiichi Uchida Yoshinori Miyamoto Nobuaki Arai Toshiharu Kakihara Takashi Yokota Junichi Okuyama Yuuki Kawabata Tohya Yasuda 《Fisheries Science》2009,75(5):1133-1140
Several species of fish exhibit strong homing ability and distinct site fidelity to their original habitats and spawning sites.
We have studied the homing ability, site fidelity, and diel movement patterns of three nocturnal black rockfish Sebastes inermis. The patterns were studied by radio-linked acoustic positioning system (VRAP) and fixed monitoring receivers (VR2) in Maizuru
Bay, Japan from June to July 2004. All tagged fish were displaced by 80–120 m, but they returned to their respective captured
sites at dusk, regardless of the time of release. The fish tagged with a depth sensor transmitter displayed active vertical
movement just after the release, and subsequently swam near the bottom depth around the captured site. After homing, they
displayed small home ranges [average 815 ± 265 m2 (SD)] around a single rocky area with one or two core areas (average 64 ± 30 m2). One core area was utilized during the daylight and dark periods, whereas the other was used primarily during the dark period.
The depth-tagged fish primarily used the depth of the rock crevice during the daylight, and it spent its dark period between
the surface and the deeper bottom depth. 相似文献
962.
Monica L. Bond Curtis M. Bradley Christian Kiffner Thomas A. Morrison Derek E. Lee 《Landscape Ecology》2017,32(8):1705-1721
Context
Managers are faced with numerous methods for delineating wildlife movement corridors, and often must make decisions with limited data. Delineated corridors should be robust to different data and models.Objectives
We present a multi-method approach for delineating and validating wildlife corridors using multiple data sources, which can be used conserve landscape connectivity. We used this approach to delineate and validate migration corridors for wildebeest (Connochaetes taurinus) in the Tarangire Ecosystem of northern Tanzania.Methods
We used two types of locational data (distance sampling detections and GPS collar locations), and three modeling methods (negative binomial regression, logistic regression, and Maxent), to generate resource selection functions (RSFs) and define resistance surfaces. We compared two corridor detection algorithms (cost-distance and circuit theory), to delineate corridors. We validated corridors by comparing random and wildebeest locations that fell within corridors, and cross-validated by data type.Results
Both data types produced similar RSFs. Wildebeest consistently selected migration habitat in flatter terrain farther from human settlements. Validation indicated three of the combinations of data type, modeling, and corridor detection algorithms (detection data with Maxent modeling, GPS collar data with logistic regression modeling, and GPS collar data with Maxent modeling, all using cost-distance) far outperformed the other seven. We merged the predictive corridors from these three data-method combinations to reveal habitat with highest probability of use.Conclusions
The use of multiple methods ensures that planning is able to prioritize conservation of migration corridors based on all available information.963.
Reyes Tárrega Leonor Calvo Ángela Taboada Elena Marcos José Antonio Marcos 《European Journal of Forest Research》2011,130(6):949-957
We compared the structure of the arboreal layer and the diversity and species composition of the understory vegetation of
three types of mature forest communities: oak (Quercus pyrenaica) and beech (Fagus sylvatica) forests and Scots pine (Pinus sylvestris) plantations. Our main aim was to determine whether differences in these variables existed and were due to the identity of
the dominant tree species. We selected four stands or replicates per forest type located geographically close and with relatively
similar conditions. We found no differences in the arboreal structure of oak and beech forests, which were characterised by
great variability in tree size, while in case of plantations, this variability was lower at both the intra-stand (estimated
by the coefficient of variation) and inter-stand (i.e. the four replicates harboured trees of similar sizes) scales. However,
the highest variability in the canopy layer of natural forests was not consistently linked to greater understory species richness.
Indeed, the lowest plant species richness was found in beech forests, while oak forests harboured the highest value at either
the sampling unit (per m2) or stand scales. The greatest negative correlation between plant diversity and the environmental variables measured was
found for litter depth, which was the highest in beech forests. The results obtained by the CCA indicated that the four replicates
of each forest type clustered together, due to the presence of characteristic species. We concluded that pine plantations
did not approach the environmental conditions of native forests, as plantations were characterised by singular understory
species composition and low arboreal layer variability, compared to natural woodlands. 相似文献
964.
Sergio Lanteri Ezio Portis Alberto Acquadro Rosario P. Mauro Giovanni Mauromicale 《Euphytica》2012,184(3):311-321
The species Cynara cardunculus includes the globe artichoke (var. scolymus), the cultivated cardoon (var. altilis) and the wild cardoon (var. sylvestris). The three taxa are sexually compatible and originate fertile F
1 progenies, which, given the high heterozygosity of the species, are highly segregating. We report the characterization of
two F
1 populations, one bred from a cross between globe artichoke and cultivated cardoon, and the other between globe artichoke
and wild cardoon. Both populations featured a wide array of phenotypes in relation to several traits, and some of the newly
developed genotypes are of interest for the ornamental market. The two populations were genotyped at 50 microsatellite (SSR)
loci: in the globe artichoke × wild cardoon and globe artichoke × cultivated cardoon progenies 116 and 97 alleles were respectively
detected. SSR pattern scores were used to produce an UPGMA dendrogram and a PCoA plot. A set of nine SSR loci, evenly dispersed
across the genome, was shown to be sufficient to unambiguously identify each segregant. The molecular fingerprinting is useful
for establishing the true to type correspondence of propagative materials in nurseries and ensures the effective correspondence
between the real and the declared identity of a clone. 相似文献
965.
Xiaojie Wang Wenming Zheng Heinrich Buchenauer Jie Zhao Qingmei Han Lili Huang Zhensheng Kang 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(3):241-247
Stripe rust of wheat caused by Puccinia striiformis f. sp. tritici is one of the most important diseases on wheat worldwide, especially in temperate regions with cool moist weather conditions.
A rapid and reliable detection of the pathogen in latent infected wheat leaves during overwintering of the fungus in the dormant
stage will contribute to determine the initial inoculum potential and thus to predict early outbreak and to improve effective
management of the disease. To achieve this aim, a PCR-based method was developed for specific and sensitive detection of P. striiformis. Specific primers were designed according to a genome-specific sequence of P. striiformis. To evaluate the specificity of the primers, seven different isolates and races of P. striiformis as well as six other pathogens of wheat were tested. All isolates of P. striiformis yielded a distinct band of a fragment of 470 bp, while using DNA of the other wheat pathogens as a template no amplification
product was detected. The sensitivity of the primers was tested using serial dilutions of total DNA from P. striiformis; the limit of detection was 10 pg of DNA. Using extracts from P. striiformis-infected wheat leaves, the fungus could be determined in the leaves before symptoms appeared. The stripe rust could also
be detected in the dormant stage by the PCR assay in samples of wheat leaves taken during the winter season. The application
of the PCR assay may be useful for rapid and reliable detection of P. striiformis in latent infected leaves of overwintering wheat plants. 相似文献
966.
Epizootic hemorrhagic disease virus (EHDV) is a vector-borne disease of ruminants disseminated in the tropic and subtropic
zone of the world. Gazella subgutturosa subgutturosa (goitered gazelle) is native species of Anatolia. Due to risk of extinction, captured gazelles have been taken under protection
in a restricted area at Ceylanpinar state farm. In this study, the presence of EHDV was investigated serologically in 82 goitered
gazelle and 399 cattle. Seropositivity rates in goitered gazelle and cattle were detected as 2.43% and 3.50%, respectively. 相似文献
967.
Pedro Soler-Lloréns Yolanda Gil-Ramírez Ana Zabalza-Baranguá Maite Iriarte Raquel Conde-álvarez Amaia Zú?iga-Ripa Beatriz San Román Michel S Zygmunt Nieves Vizcaíno Axel Cloeckaert María-Jesús Grilló Ignacio Moriyón Ignacio López-Go?i 《Veterinary research》2014,45(1):72
Brucella spp. are Gram-negative bacteria that behave as facultative intracellular parasites of a variety of mammals. This genus includes smooth (S) and rough (R) species that carry S and R lipopolysaccharides (LPS), respectively. S-LPS is a virulence factor, and mutants affected in the S-LPS O-polysaccharide (R mutants), core oligosaccharide or both show attenuation. However, B. ovis is naturally R and is virulent in sheep. We studied the role of B. ovis LPS in virulence by mutating the orthologues of wadA, wadB and wadC, three genes known to encode LPS core glycosyltransferases in S brucellae. When mapped with antibodies to outer membrane proteins (Omps) and R-LPS, wadB and wadC mutants displayed defects in LPS structure and outer membrane topology but inactivation of wadA had little or no effect. Consistent with these observations, the wadB and wadC but not the wadA mutants were attenuated in mice. When tested as vaccines, the wadB and wadC mutants protected mice against B. ovis challenge. The results demonstrate that the LPS core is a structure essential for survival in vivo not only of S brucellae but also of a naturally R Brucella pathogenic species, and they confirm our previous hypothesis that the Brucella LPS core is a target for vaccine development. Since vaccine B. melitensis Rev 1 is S and thus interferes in serological testing for S brucellae, wadB mutant represents a candidate vaccine to be evaluated against B. ovis infection of sheep suitable for areas free of B. melitensis. 相似文献
968.
Faba bean (Vicia faba L.) has high utility as a food and soil fertility improving crop. One of the major fungal pathogens of faba bean is Botrytis fabae, the causative agent of chocolate spot. The disease affects significantly the leaf, stem, pod and seed of faba bean compromise its productivity in the smallholder farming sector. Nonetheless, there are limited resistant/tolerant faba bean varieties available and disease control technology options. Therefore, it was prudent to evaluate faba bean landraces for chocolate spot resistance. Fifty landraces together with ten improved varieties were evaluated both in the field and in the greenhouse under natural and artificial inoculation with previously selected aggressive Botrytis fabae isolate (Iso-016) from West Gojjam, in Ethiopia. There were highly significant differences (p?<?0.001) among the landraces for reaction to the disease and agronomic traits. Significant positive correlation was recorded between reaction of genotypes in the field and greenhouse disease data. The overall mean disease epidemics varied from 92.5 to 697.5 for the area under disease progress curve (AUDPC). The highest level of resistance was found in the ICARDA lines, ILB-4726, ILB-938 and BPL-710. Of all 18 landrace collections displayed significantly lower disease reaction than the susceptible check. However the resistance was moderate. The selected eighteen landraces will be recommended for use in breeding for chocolate resistance. Overall, resistance was highly heritable, suggesting that phenotypic selection can be exploited to improve chocolate spot resistance in faba bean varieties. 相似文献
969.
The effects of starvation on biochemical composition and gametogenesis were investigated in the Pacific oyster Crassostrea gigas. Histological analysis, combined with oocyte examination and measurements of protein, glycogen and lipid levels and RNA/DNA
ratio from gonad, adductor muscle and mantle tissue of each sex were performed. In the starved groups, C. gigas showed gonad development, but the progress was delayed during the experiment. Glycogen was the first substrate used by C. gigas for dealing with lack of food. While glycogen was rapidly consumed, protein and lipid contents decreased gradually. A decrease
in the RNA/DNA ratio in the starved groups in all the body components was found during starvation, illustrating that RNA/DNA
ratio was a valid indicator of nutritional condition in C. gigas. A significant increase in water and ash contents and a corresponding decrease in condition index were observed in the starved
groups, showing that the water and ash content and condition index were related to the usage of glycogen, lipid and protein
reserves in body composition. During starvation, energy reserves were mobilized for survival and gonad development, but spawning
was arrested. The information obtained in this study is useful for broodstock management in the Pacific oyster industry. 相似文献
970.
Ai Jin Cai-xia Lei Jing-jing Tian Jian Sun Hong Ji 《Fish physiology and biochemistry》2018,44(1):197-207
The purpose of this study was to explore the mechanism of by which docosahexaenoic acid (DHA) inhibit the accumulation of adipose tissue lipid in grass carp (Ctenopharyngodon idella). We therefore designed two semi-purified diets, namely DHA-free (control) and DHA-supplemented, and fed them to grass carp (22.19 ± 1.76 g) for 3 and 6 weeks. DHA supplementation led to a significantly lower intraperitoneal fat index (IPFI) than that in the control group by reducing the number of adipocytes but significantly higher adipocyte size (P < 0.05). In the intraperitoneal adipose tissue, the DHA-fed group showed significantly higher peroxisome proliferator-activated receptor (PPAR)γ, CCAAT enhancer-binding protein (C/EBP)α, and sterol regulatory element-binding protein (SREBP)1c mRNA expression levels at both 3 and 6 weeks (P < 0.05). However, the ratio of the expression levels of B cell leukemia 2 (Bcl-2) and Bcl-2-associated X protein (Bax) was significantly lower in the DHA-fed group than in the control group (P < 0.05), and the protein expression levels of the apoptosis-related proteins caspase 3, caspase 8, and caspase 9 were also significantly higher (P < 0.05). Overall, although DHA promotes lipid synthesis, it is more likely that DHA could suppress the lipid accumulation in adipocytes of grass carp by inducing adipocyte apoptosis. 相似文献