哈茨木霉天冬氨酸蛋白酶基因SA76的3＇RACE扩增和序列分析

由EST获得全长cDNA对于结构基因组学和功能基因组学都是至关重要的,cDNA末端快速扩增技术RACE是该领域中的重要研究方法.利用BD SMART RACE技术扩增编码分泌天冬氨酸蛋白酶SA76基因的3＇末端,将其与哈茨木霉cDNA文库中的SA76基因的EST序列进行序列拼接,获得2019bp的全长cDNA序列,其开放读码框长1593bp,5＇非编码区266bp,3＇非编码区201bp,编码530个氨基酸,有信号肽.哈茨木霉天冬氨酸蛋白酶基因与玉蜀黍赤霉、粗糙脉孢菌、球毛壳菌天冬氨酸蛋白酶基因的同源性分别为53%, 37%, 36%.利用BD SMART RACE技术首次从哈茨木霉中克隆天冬氨酸蛋白酶基因,为验证SA76基因的功能奠定基础,为进一步研究蛋白酶的作用机制及生物防治功能提供依据.     

Socio-economic significance of reed forests in a rural community: A case study from the greater Sylhet Region of Bangladesh

The reed forests that are the subject of this study are scattered over five thanas of the Sunamganj and Sylhet Districts of the Sylhet Division of Bangladesh. Their total area is 23,590 ha and they have great ecological, economic, commercial and socio-economic importance due to the diversified resources they supply. However, the forests are commonly encroached on by local people who are perceived to be seriously depleting the resource. This paper deals with the socio-economic status of the people residing near these reed forests, and examines their dependency on the resources provided by the reed forests. A socio-economic survey, participatory rural appraisal and interviews were used to obtain baseline data of the reed forest resource and the local communities. The study reveals that the communities in the study region have higher income than other areas of Bangladesh. Most survey respondents have other occupations in addition to farming. The literacy rate is 28.8%. Among the illiterate, 68% engage in collecting reeds. Although the Forest Department has a management plan for sustainable use of the reed forests, encroachers receive backing from political leaders and local elites, so their eviction is difficult. In order to achieve long-term productivity and sustainability from the reed forests, this study recommends a strategy of developing an integrated joint management plan between the Forest Department and the local people.     

The use of compensatory and non-compensatory multi-criteria analysis for small-scale forestry

Small-scale farm forestry has the potential to offer many benefits both to landholders and the wider community. As with all changes in land-use practices, there are associated benefits and costs and these are not uniformly distributed. They have varying impacts on the different values, aspirations, goals and objectives that exist within the community. Furthermore, the community does not consider these values, aspirations, goals and objectives of equal importance. The degree of concern can vary from minor to high and overriding all other considerations. When evaluating farm forestry options it is necessary to address all of these concerns. This paper examines the combined use compensatory and non-compensatory multi-criteria analyses to evaluate forestry options, in a case study for the Darling Downs region of Queensland, Australia. These aggregation techniques are found to be highly complementary and together provide a comprehensive analysis. The compensatory technique provides a sound measure of overall performance of a forestry system, whereas the non-compensatory technique alerts decision-makers to presence of particularly poor performance with respect to individual criteria. The compensatory technique used is simple and understandable even for those with non-mathematical backgrounds. This analysis can identify and aid communication of the relative benefits and costs, and trade-offs, between economic, environmental and social considerations.     

Predicting moisture content and density distribution of Scots pine by microwave scanning of sawn timber

This study was carried out to investigate the possibility of calibrating a prediction model for the moisture content and density distribution of Scots pine (Pinus sylvestris) using microwave sensors. The material was initially of green moisture content and was thereafter dried in several steps to zero moisture content. At each step, all the pieces were weighed, scanned with a microwave sensor (Satimo 9,4GHz), and computed tomography (CT)-scanned with a medical CT scanner (Siemens Somatom AR.T.). The output variables from the microwave sensor were used as predictors, and CT images that correlated with known moisture content were used as response variables. Multivariate models to predict average moisture content and density were calibrated using the partial least squares (PLS) regression. The models for average moisture content and density were applied at the pixel level, and the distribution was visualized. The results show that it is possible to predict both moisture content distribution and density distribution with high accuracy using microwave sensors.     

Radial patterns of sap flow in woody stems of dominant and understory species: scaling errors associated with positioning of sensors

We studied sap flow in dominant coniferous (Pinus sylvestris L.) and broadleaf (Populus canescens L.) species and in understory species (Prunus serotina Ehrh. and Rhododendron ponticum L.) by the heat field deformation (HFD) method. We attempted to identify possible errors arising during flow integration and scaling from single-point measurements to whole trees. Large systematic errors of -90 to 300% were found when it was assumed that sap flow was uniform over the sapwood depth. Therefore, we recommend that the radial sap flow pattern should be determined first using sensors with multiple measuring points along a stem radius followed by single-point measurements with sensors placed at a predetermined depth. Other significant errors occurred in the scaling procedure even when the sap flow radial pattern was known. These included errors associated with uncertainties in the positioning of sensors beneath the cambium (up to 15% per 1 mm error in estimated xylem depth), and differences in environmental conditions when the radial profile applied for integration was determined over the short term (up to 47% error). High temporal variation in the point-to-area correction factor along the xylem radius used for flow integration is also problematic. Compared with midday measurements, measurements of radial variation of sap flow in the morning and evening of sunny days minimized the influence of temporal variations on the point-to-area correction factor, which was especially pronounced in trees with a highly asymmetric sap flow radial pattern because of differences in functioning of the sapwood xylem layers. Positioning a single-point sensor at a depth with maximum sap flow is advantageous because of the high sensitivity of maximum sap flow to water stress conditions and changes in micro-climate, and because of the lower random errors associated with the positioning of a single-point sensor along the xylem radius.     

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Comparison of seasonal responses ofHylobius abietis (L.) (Col., Curculionidae) to chemical and natural lures in baited pitfall traps

Differences in catches ofHylobius abietis (L.) adults in baited pitfall traps with chemical (alpha-pinene, ethanol, water) and natural (spruce billets, water) lures were found not in the over-all number of trapped adult weevils, but in the per cent occurrence and number of old and young beetles in individual periods of the season.     

<Emphasis Type="Italic">Pinus halepensis</Emphasis> somatic embryogenesis is affected by the physical and chemical conditions at the initial stages of the process

Pinus halepensis has been described as a drought-tolerant species with high plasticity to growth in different environments. Its eco-physiological characteristics could facilitate the use of this species in large afforestations in the future scenery of climate change. Somatic embryogenesis is a biotechnological tool with potential for large-scale clonal propagation. In order to establish an improved regeneration protocol for Pinus halepensis, the effects of different temperatures (18, 23, and 28 °C) and water availability conditions (2, 3, and 4 g L^?1 Gelrite^®), during initiation of embryonal masses on the rate of initiation, proliferation, maturation, and the number of embryos developed, were evaluated. It was found that environmental conditions during the initiation stage of Pinus halepensis somatic embryogenesis influence the success of initiation and proliferation. In contrast, there was no effect of these conditions on the maturation rates and the number of somatic embryos. Somatic embryos were obtained in all treatments tested, indicating that plants can be produced from extreme conditions of induction, such as high temperatures (28 °C) and low water availability conditions (4 g L^?1).