Biological, chemical and thermal indices of soil organic matter stability in four grassland soils

The various ecosystem functions of soil organic matter (SOM) depend on both its quantity and stability. Numerous fractionation techniques have been developed to characterize SOM stability, and thermal analysis techniques have shown promising results to describe the complete continuum of SOM in whole soil samples. However, the potential link between SOM thermal stability and biological or chemical stability has not yet been adequately explored. The objective of this study was to compare conventional chemical and biological methods used to characterize SOM stability with results obtained by thermal analysis techniques. Surface soil samples were collected from four North American grassland sites along a continental mean annual temperature gradient, each with a native and cultivated land use. Soil organic C concentrations ranged from 6.8 to 33 g C kg⁻¹ soil. Soils were incubated for 588 days at 35 °C, and C mineralization rates were determined periodically throughout the incubation by measuring CO₂ concentration using an infrared gas analyzer (IRGA) to calculate biological indices of SOM stability. Hot-water extractable organic C (HWEOC) contents were determined before and after incubation as chemical indices. Finally, samples from before and after incubation were analyzed by simultaneous thermal analysis (i.e., thermogravimetry (TG) and differential scanning calorimetry (DSC)) to determine thermal indices of SOM stability. Long-term incubation resulted in the mineralization of up to 33% of initial soil C. The number of days required to respire 5% of initial soil organic carbon (SOC), ranged from 27 to 115 days, and is proposed as a standardized biological index of SOM stability. The number of days was greater for cultivated soils compared to soils under native vegetation, and generally decreased with increasing site mean annual temperature. HWEOC (as % of initial SOC) did not show consistent responses to land use, but was significantly lower after long-term incubation. Energy density (J mg⁻¹ OM) was greater for soils under native vegetation compared to cultivated soils, and long-term incubation also decreased energy density. The temperatures at which half of the mass loss or energy release occurred typically showed larger responses to land use change than to incubation. Strong correlations demonstrated a link between the thermal and biogeochemical stability of SOM, but the interpretation of the thermal behavior of SOM in bulk soil samples remains equivocal because of the role the mineral component and organo-mineral interactions.     

River bed restoration boosts habitat mosaics and the demography of two rare non-aquatic vertebrates

Many river revitalization projects are currently under development, but we still lack proper assessments of their effects on biodiversity, especially regarding non-aquatic vertebrates. We analysed the progressive changes in habitat mosaics, and the demographic response of two rare species of wading birds along a Swiss section of the Rhone river over a period of three decades. During the period preceding revitalization, the diversity of the habitat mosaic had been steadily declining. This impoverishment could however be reverted after 1994, when the revitalization was initiated: in 2006, the re-established habitat matrix was more diverse than at any time during the previous 27 years. The formerly channelized stream underwent radical transformation as it mutated into a heterogeneous braided river boosting pioneer riverine habitats. This has led the two wading bird species to establish significantly more territories than before the revitalization operations. Population size of Actitis hypoleucos and Charadrius dubius increased by 83% and 20%, respectively, between 1995 and 2007. During the same period, A. hypoleucos declined dramatically in Switzerland as a whole, whilst C. dubius showed no distinct population trend. The response of the two waders may have resulted from an increase in the number of lateral arms of the river, where the availability of productive temporary ponds provides excellent foraging opportunities. A significant proportion of these habitats was created by a targeted extraction of sediments with the objective to promote biodiversity while maintaining economic activity and improving security against floods. This suggests that gravel exploitation is not incompatible with efficient river restoration.     

Water loss regulation in mature Hevea brasiliensis: effects of intermittent drought in the rainy season and hydraulic regulation

Effects of soil and atmospheric drought on whole-tree transpiration (E(T)), leaf water potential (Ψ(L)) and whole-tree hydraulic conductance (K(T)) were investigated in mature rubber trees (Hevea brasiliensis, clone RRIM 600) during the full canopy stage in the rainy season in a drought-prone area of northeast Thailand. Under well-watered soil conditions, transpiration was tightly regulated in response to high evaporative demand, i.e., above reference evapotranspiration (ET(0)) ~2.2 mm day(-1) or maximum vapor pressure deficit ~1.8 kPa. When the trees experienced intermittent soil drought E(T) decreased sharply when relative extractable water in the top soil was?相似文献   

Unexpected seedling growth in the understory of post-agricultural forests from Eastern Canada

•Introduction  

The agricultural landscape in Eastern Canada has drastically changed in the last decades. Whilst certain forest communities are disappearing, post-agricultural forests are emerging from old fields colonized by fast-growing tree species.     

Development and evaluation of a new method for sampling and monitoring the symphylid population in pineapple

BACKGROUND: Symphylids (Hanseniella sp.) are polyphagous soilborne parasites. Today, symphylid populations on pineapple are monitored by observing root symptoms and the presence of symphylids at the bottom of basal leaves. The authors developed a reliable method with a bait and trap device to monitor symphylid populations in pineapple or fallow crops. The spatial distribution of the symphylid populations was evaluated using the variance/mean ratios and spatial analyses based on Moran's and Geary's indices. The method has been tested to monitor symphylid populations at different developmental stages of pineapple. RESULTS: Adding potato baits to the soil samples increased the trapping efficiency of symphylids when compared with ‘soil only’ and ‘bait only’ methods. The handling of the samples is also facilitated by the new device. Results showed that the vertical distribution of symphylids may be uniform deeply inside the soil profile under pineapple, up to 50 cm. Results showed that symphylid populations are highly aggregated, showing a spot area about 4–6 m wide for their development. CONCLUSION: The new method allows better and easier evaluation of symphylid populations. It may be very useful in the evaluation of new IPM methods to control symphylids under pineapple. Copyright © 2011 Society of Chemical Industry     

A side effect of decreased fertility associated with vaccination against bluetongue virus serotype 8 in Holstein dairy cows

Inactivated virus vaccines have been widely used to control bluetongue after introduction of serotype 8 of the bluetongue virus (BTV) in northern Europe in 2006. To evaluate vaccination, quantitative knowledge of its possible side effects is needed. One current adverse reaction with inactivated vaccines is a rise in body temperature, which could reduce cow reproductive performance. The objective of this study was to quantify a possible side effect of vaccination on fertility before the implantation of the embryo of dairy cows under field conditions. The study was performed on herds that were not exposed to BTV. Fertility was assessed by return-to-service following artificial insemination (AI). Biological assumptions for a possible side effect of vaccination were conception failure and embryonic death. Associations between return-to-service rates and vaccine injections were assessed using mixed-logistic regression models and survival analysis. Two models were considered: a 3-week-return-to-service model comparing cows vaccinated between 3 days before and 16 days after AI and unvaccinated cows (assuming an effect on conception failure or early embryonic death), and a 90-day-return-to-service model comparing cows vaccinated between 3 days before and 42 days after AI and unvaccinated cows (assuming an effect on conception failure, early or late embryonic death). Only cows receiving a second vaccine injection between 2 and 7 days after AI had a significantly higher risk of 3-week-return-to-service (RR=1.19 [1.07-1.33]). This corresponds to an increase of return-to-service by 4 percentage points. A side effect of vaccination could be due to early embryonic death. The slight side effect on fertility associated with vaccination was low compared to effects of BTV-8 exposure on fertility.     

The invasion process of bovine erythrocyte by Babesia divergens: knowledge from an in vitro assay

ABSTRACT: Babesia divergens is a tick-transmitted apicomplexan parasite for which asexual multiplication in its vertebrate hosts is restricted to erythrocytes. Current knowledge of invasion of these target cells is limited. An efficient in vitro invasion assay was set up to gain access to this information. Parasites prepared from infected RBC, lysed by electroporation, and mixed with bovine RBC in a selected synthetic medium (RPMI 1640 supplemented with calcium) were able to establish subsequent cultures with parasitemia ranging from 6 to 14%. Free parasites remaining in the invasion medium could be eliminated by Percoll gradient and culture could be pursued with the freshly invaded erythrocytes. In this way, the invasion time window could be shortened to obtain a synchronised start of the culture or to study the kinetics of invasion. With this assay we demonstrate that 1) erythrocyte invasion by B. divergens is a rapid process since 70% of the invasion-competent parasites invaded the RBC in less than 45 s; 2) all invasion-competent parasites achieved invasion within 10 min of contact; 3) one erythrocyte could be invaded concomitantly by two merozoites; 4) despite a synchronous start, the parasite population evolved heterogeneously resulting in a progressive loss of synchronisation. Western blot analysis of proteins collected from invasion medium were performed with sera from animals experimentally infected with B. divergens and highlighted several proteins. The dose-dependent, inhibitory effects of these sera on B. divergens invasion suggest that these proteins might be involved in the invasion process. Further investigations are required for their characterisation.     

<Emphasis Type="Italic">Allanblackia floribunda</Emphasis>: a new oil tree crop for Africa: amenability to grafting

Three Allanblackia species (A. floribunda, A. stuhlmannii and A. parviflora) with high nutritive, medicinal, cosmetic and economic values are currently being domesticated as new oil tree crops. Allanblackia seeds contain a hard white fat consisting mostly of stearic (52–58%) and oleic (39–49%) acids. This unusual fatty acid content has the right properties for many different food and cosmetic products making them commercially interesting. Vegetative propagation studies on A. floribunda, which grows naturally in the moist forest of Cameroon and Nigeria, were initiated aimed at evaluating its amenability to grafting. Scions were grafted onto 18 month old rootstocks of A. floribunda using side tongue, top cleft, side veneer, whip-and-tongue methods under nursery conditions in Cameroon. In parallel, side tongue and inverted ‘T’ budding methods were also tested in situ on young A. floribunda wildings growing under semi-deciduous and evergreen tree covers. In addition, the effects of protecting side tongue new grafts with non perforated translucent plastic, perforated translucent plastic and aluminium foil were assessed. Under nursery conditions, side tongue grafts were significantly more successful (80.0 ± 6.3%), than grafts of side veneer (52.5 ± 7.9%), top cleft (55.0 ± 7.9%) and whip and tongue (50.0 ± 7.9%). The success of side tongue graft was further increased (86.7 ± 6.2%) under the shade of evergreen trees when protected by non perforated translucent plastic. These results indicate the potential for in situ grafting and ‘top working’ to promote cultivation of more productive germplasm of Allanblackia within multifunctional agricultural systems.     

Long‐term survival with stereotactic radiotherapy for imaging‐diagnosed pituitary tumors in dogs

Published studies on the use of stereotactic radiotherapy for dogs with pituitary tumors are limited. This retrospective observational study describes results of stereotactic radiotherapy for 45 dogs with imaging‐diagnosed pituitary tumors. All dogs were treated at a single hospital during the period of December 2009–2015. The stereotactic radiotherapy was delivered in one 15 Gray (Gy) fraction or in three 8 Gy fractions. At the time of analysis, 41 dogs were deceased. Four were alive and censored from all survival analyses; one dog received 8 Gy every other day and was removed from protocol analyses. The median overall survival from first treatment was 311 days (95% confidence interval 226–410 days [range 1–2134 days]). Thirty‐two dogs received 15 Gy (median overall survival 311 days; 95% confidence interval [range 221–427 days]), and 12 received 24 Gy on three consecutive days (median overall survival 245 days, 95% confidence interval [range 2–626 days]). Twenty‐nine dogs had hyperadrenocorticism (median overall survival 245 days), while 16 had nonfunctional masses (median overall survival 626 days). Clinical improvement was reported in 37/45 cases. Presumptive signs of acute adverse effects within 4 months of stereotactic radiotherapy were noted in 10/45, and most had improvement spontaneously or with steroids. Late effects versus tumor progression were not discernable, but posttreatment blindness (2), hypernatremia (2), and progressive neurological signs (31) were reported. There was no statistical difference in median overall survival for different protocols. Patients with nonfunctional masses had longer median overall survival than those with hyperadrenocorticism (P = 0.0003). Survival outcomes with stereotactic radiotherapy were shorter than those previously reported with definitive radiation, especially for dogs with hyperadrenocorticism.