Effect of tropical browse leaves supplementation on rumen enzymes of sheep and goats fed <Emphasis Type="Italic">Dichanthium annulatum</Emphasis> grass-based diets

In a switch-over experiment, eight male animals, four each of sheep and goats of local breeds with mean body weight of 26. 8 ± 2.0 and 30.0 ± 2.1 kg, were fed Dichanthium annulatum (DA) grass and four browse species viz. Helictris isora, Securengia virosa, Leucaena leucocephala (LL) and Hardwickia binnata (HB) in four feeding trials to assess their supplementary effect on activity of rumen enzymes. The sheep and goats were offered DA grass with individual browse in 75:25 and 50:50 proportions, respectively, for more than 3 months during each feeding trial, and rumen liquor samples were collected twice at 0 and 4 h post feeding after 60 and 90 days of feeding. Glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and glutamate dehydrogenase (GDH) enzymes were determined in the bacteria and protozoa fractions of rumen liquor, while cellulase enzyme activity was measured in mixed rumen liquor. LL and HB had the highest and lowest contents of CP, while fibre contents were lower in early than later browse leaves. Supplementation of browse leaves significantly (P < 0.05) affect the specific activity of GDH enzyme in bacteria fraction of rumen liquor of animal species, while GDH activity was similar in protozoa fraction of rumen liquor of sheep and goats on all DA grass–browse-supplemented diets except DA–HB (42.8 units/mg protein), where activity was significantly (P < 0.05) low. Specific activities of GOT and GPT enzymes in both bacteria and protozoa fractions of rumen liquor differ significantly (P < 0.05) due to supplementation of browse leaves to DA grass. Browse leaves significantly (P < 0.05) affect the cellulase enzyme activity in animal rumen liquor, being highest on DA–LL (193.4) and lowest on DA–HB diet (144.8 μg sugar/mg protein). Goat exhibited higher activities of GOT and GPT than sheep in both bacteria and protozoa fraction of rumen liquor, while cellulase activity was similar between the animal species on the grass–browse leaves diets. Results indicate that browse leaves supplementation affect the enzyme activities of sheep and goats rumen, while the goats rumen liquor had higher activities of GOT, GPT and GDH enzyme than sheep.     

Bioresmethrin-induced alterations in the ultrastructure of neurosecretory cells of insect corpora cardiaca

The ultrastructure of the neurosecretory cells of the corpora cardiaca (CC) of Locusta migratoria and Rhodnius prolixus was altered following incubation of the isolated glands in 1 μM bioresmethrin. The insecticide induced formation of large vacuoles in the soma and extensive exocytosis in the axon terminals of treated cells. Pretreatment of the CC with 1 μM tetrodotoxin blocked the action of bioresmethrin. Bioresmethrin affected the distribution and shape of mitochondria in neurosecretory cells. In Rhodnius, numerous mitochondria were found clustered in vacuoles in neurosecretory cells of the CC treated with bioresmethrin. Some of these mitochondria were abnormally elongated while others showed signs of fission. The results are discussed in relation to the action of bioresmethrin on neurosecretory cells.     

An optimised protocol for molecular identification of Eimeria from chickens

Molecular approaches supporting identification of Eimeria parasites infecting chickens have been available for more than 20 years, although they have largely failed to replace traditional measures such as microscopy and pathology. Limitations of microscopy-led diagnostics, including a requirement for specialist parasitological expertise and low sample throughput, are yet to be outweighed by the difficulties associated with accessing genomic DNA from environmental Eimeria samples. A key step towards the use of Eimeria species-specific PCR as a sensitive and reproducible discriminatory tool for use in the field is the production of a standardised protocol that includes sample collection and DNA template preparation, as well as primer selection from the numerous PCR assays now published. Such a protocol will facilitate development of valuable epidemiological datasets which may be easily compared between studies and laboratories. The outcome of an optimisation process undertaken in laboratories in India and the UK is described here, identifying four steps. First, samples were collected into a 2% (w/v) potassium dichromate solution. Second, oocysts were enriched by flotation in saturated saline. Third, genomic DNA was extracted using a QIAamp DNA Stool mini kit protocol including a mechanical homogenisation step. Finally, nested PCR was carried out using previously published primers targeting the internal transcribed spacer region 1 (ITS-1). Alternative methods tested included sample processing in the presence of faecal material, DNA extraction using a traditional phenol/chloroform protocol, the use of SCAR multiplex PCR (one tube and two tube versions) and speciation using the morphometric tool COCCIMORPH for the first time with field samples.     

Flavoring Extracts of Hemidesmus indicus Roots and Vanilla planifolia Pods Exhibit In vitro Acetylcholinesterase Inhibitory Activities

Acetylcholinesterase inhibitors (AChEIs) are important for treatment of Alzheimer’s disease and other neurological disorders. Search for potent and safe AChEIs from plant sources still continues. In the present work, we explored fragrant plant extracts that are traditionally used in flavoring foods, namely, Hemidesmus indicus and Vanilla planifolia, as possible sources for AChEI. Root and pod extracts of H. indicus and V. planifolia, respectively, produce fragrant phenolic compounds, 2-hydroxy-4-methoxybenzaldehyde (MBALD) and 4-hydroxy-3-methoxybenzaldehyde (vanillin). These methoxybenzaldehydes were shown to have inhibitory potential against acetylcholinesterase (AChE). Vanillin (IC₅₀?=?0.037 mM) was detected as more efficient inhibitor than MBALD (IC₅₀?=?0.047 mM). This finding was supported by kinetic analysis. Thus, plant-based food flavoring agents showed capacity in curing Alzheimer’s disease and other neurological dysfunctions.     

The sun and nearby stars: microwave observations at high resolution

High-resolution microwave observations are providing new insights into the nature of active regions and eruptions on the sun and nearby stars. The strength, evolution, and structure of magnetic fields in coronal loops can be determined by multiple-wavelength observations with the Very Large Array. Flare models can be tested with Very Large Array snapshot maps, which have angular resolutions of better than 1 second of arc in time periods as short as 10 seconds. Magnetic changes that precede solar eruptions on time scales of tens of minutes involve primarily emerging coronal loops and the interactions of two or more loops. Magnetic reconnection at the interface of two closed loops may accelerate electrons and trigger the release of microwave energy in the coronal parts of the magnetic loops. Nearby main-sequence stars of late spectral type emit slowly varying microwave radiation and stellar microwave bursts that show striking similarities to those of the sun.     

The repair of segmental bone defects with porous bioglass: an experimental study in goat

This study was exclusively conducted to evaluate healing of surgically created defects on the radius of adult Black Bengal goat after implantation of porous bioglass blocks and compare the process kinetics with normal healing. Twelve Black Bengal goats were divided randomly into two groups: control and experimental group implanted with bioglass blocks. Unicortical bone defects in radius were generated in all animals under aseptic condition. Local inflammatory reaction and healing of wound, radiological investigations, histological studies, oxytetracycline leveling and angiographic studies were performed up to 90th day post-operatively and compared with normal healing. It has been found that extensive new bone formation originating from host bone towards the implant whereas in control, the process was active from both the ends; the defect site appeared as homogenous nonfluorescent area. Thus, porous bioglass promoted bone formation over the entire extension of the defect independent of size of block in comparison to control group.     

Comparative analysis of seed morphometric and allozyme data among four populations of neem (Azadirachta indica)

The objectives of this study were to determine the patterns of variability and to compare genetic distance between seed morphometric and allozyme data of neem (Azadirachta indica). Phenotypic variation from open pollinated seed materials at 12 isozyme loci was examined using starch gel electrophoresis for four populations from Thailand, Bangladesh and Kenya. All four populations exhibited high levels of variation for seed parameters and isozymes. UPGMA cluster analysis of Nei's genetic distance when based on isozymes revealed two genetic groups. The population from Kenya showed a close genetic relationship with the Bangladeshi populations. The population from Thailand exhibited a distant relationship with the other three populations. This result suggests that the Kenyan population was introduced from the Indian subcontinent. In contrast, cluster analysis of seed morphometric data indicated three different groups of populations. The population from Kenya showed a distant relationship with all other populations. The study demonstrates that allozyme data may not correspond with morphometric traits when measuring genetic distance. It is suggested that this controversy may depend on environmental influence on morphometric traits, possibly also on the enzyme patterns.     

Different indices to characterize water use pattern of irrigated cauliflower (Brassica oleracea L. var. botrytis) in a hot sub-humid climate of India

Frequency and depth of irrigation play crucial role in crop yield and use efficiency of water resource. To test this hypothesis a field study was carried out in November to January of 2001-2002 to 2003-2004 on a sandy loam (Aeric haplaquept) for quantifying the frequency and depth of irrigation on growth, curd yield (CY) and water use pattern of cauliflower (Brassica oleracea L. var. botrytis). Four irrigation frequencies depending on the attainment of cumulative pan evaporation (CPE) values of: 25 (CPE₂₅), 31(CPE₃₁), 38 (CPE₃₈) and 45 (CPE₄₅) mm were placed in main-plots, with three depth of irrigation (IW) of 35 (IW₃₅), 30 (IW₃₀) and 25 (IW₂₅) mm in sub-plots. Water use efficiency (WUE), net evapotranspiration efficiency (WUE_ET) and irrigation water use efficiency (WUE_I) were computed. Marginal water use efficiency (MWUE) and elasticity of water productivity (EWP) were calculated using the relationship between CY and seasonal actual evapotranspiration (SET). A continuous increasing trend in growth parameters, yield and WUE_I was recorded with the increase in SET from CPE₄₅-IW₂₅ to CPE₃₁-IW₃₀. However with further increase in SET the same decreased up to CPE₂₅-IW₃₅ regime. Highest WUE and WUE_ET obtained under CPE₃₈-IW₃₅ regime where SET value was 5% lower than the status of SET under CPE₃₁-IW₃₀. This study confirmed that critical levels of SET needed to obtain maximum curd yield or WUE, could be obtained more precisely from the knowledge of MWUE and EWP.     

Soil Carbon Sequestration in Long-Term Fertilization Under Jute-Rice-Wheat Agro-Ecosystem

Soil organic carbon (SOC) sequestration in response to long-term fertilizer management practices under jute-rice-wheat agro-ecosystem in alluvial soils was studied using a modeling approach. Fertilizer management practices included nitrogen (N), phosphorus (P) and potassium (K) fertilization, manure application, and root-stubble retention of all three crops. Soil carbon (C) model RothC was used to simulate the critical C input rates needed to maintain initial soil C level in long timescale (44 years). SOC change was significantly influenced by the long-term fertilizer management practices and the edaphic variable of initial SOC content. The effects of fertilizer combination “100%NPK+FYM” on SOC changes were most significant over “100%NPK” fertilization. If the 100% NPK fertilizer along with manure applied with stubble and roots retention of all crops, alluvial soils of such agro-ecosystem would act as a net C sink, and the average SOC density kept increasing from 18.18 Mg ha^?1 during 1972 to the current average of ～22 Mg ha^?1 during 2065 s. On an average, the critical C input was estimated to be 5.30 Mg C ha^?1 yr^?1, depending on local soil and climatic conditions. The critical C input could be effectively estimated using a summary model driven by current SOC level, mean annual temperature, precipitation, and soil clay content. Such information will provide a baseline for assessing soil C dynamics under potential changes in fertilizer and crop residues management practices, and thus enable development of management strategies for effectively mitigating climate change through soil C sequestration.     

Long-term yield trend and sustainability of rainfed soybean–wheat system through farmyard manure application in a sandy loam soil of the Indian Himalayas

A long-term (30 years) soybean–wheat experiment was conducted at Hawalbagh, Almora, India to study the effects of organic and inorganic sources of nutrients on grain yield trends of rainfed soybean (Glycine max)–wheat (Triticum aestivum) system and nutrient status (soil C, N, P and K) in a sandy loam soil (Typic Haplaquept). The unfertilized plot supported 0.56 Mg ha⁻¹ of soybean yield and 0.71 Mg ha⁻¹ of wheat yield (average yield of 30 years). Soybean responded to inorganic NPK application and the yield increased significantly to 0.87 Mg ha⁻¹ with NPK. Maximum yields of soybean (2.84 Mg ha⁻¹) and residual wheat (1.88 Mg ha⁻¹) were obtained in the plots under NPK + farmyard manure (FYM) treatment, which were significantly higher than yields observed under other treatments. Soybean yields in the plots under the unfertilized and the inorganic fertilizer treatments decreased with time, whereas yields increased significantly in the plots under N + FYM and NPK + FYM treatments. At the end of 30 years, total soil organic C (SOC) and total N concentrations increased in all the treatments. Soils under NPK + FYM-treated plots contained higher SOC and total N by 89 and 58% in the 0–45 cm soil layer, respectively, over that of the initial status. Hence, the decline in yields might be due to decline in available P and K status of soil. Combined use of NPK and FYM increased SOC, oxidizable SOC, total N, total P, Olsen P, and ammonium acetate exchangeable K by 37.8, 42.0, 20.8, 30.2, 25.0, and 52.7%, respectively, at 0–45 cm soil layer compared to application of NPK through inorganic fertilizers. However, the soil profiles under all the treatments had a net loss of nonexchangeable K, ranging from 172 kg ha⁻¹ under treatment NK to a maximum of 960 kg ha⁻¹ under NPK + FYM after 30 years of cropping. Depletion of available P and K might have contributed to the soybean yield decline in treatments where manure was not applied. The study also showed that although the combined NPK and FYM application sustained long-term productivity of the soybean–wheat system, increased K input is required to maintain soil nonexchangeable K level.