Comparison between fertilizers and untreated sewage effluent as sources for nutrient recovery by wheat (Triticum aestivum)-soybean (Glycine max) cropping system

Recovery and recycling of plant nutrients from municipal sewage effluent (SE) can reduce consumption of costly chemical fertilizers, besides reducing eutrophication of water bodies. Field experiment was conducted on Vertisol of central India for six years with the objective of quantifying recovery of major plant nutrients by aboveground biomass of wheat-soybean cropping system from untreated SE. Wheat crop was grown with irrigation (groundwater and sewage effluent) and fertilizer treatments; while soybean was grown without any treatments. Recoveries of nitrogen (N), phosphorus P) and potassium (K) by the aboveground biomass were considerably more from SE than from fertilizers and manures. Recovery of nutrients from SE was the highest by wheat grain for N and by soybean straw for P and K. Straw biomass of both the crops recovered about 31% N, 22% P and 69% K from SE, which can be recycled back into agricultural land of groundwater (GW) irrigated as well as rainfed area.     

Optimizing Seed Sample Size for Zinc and Iron Analysis of Wild and Cultivated Lentil

Zinc and iron deficiencies affect billions of people globally and have direct impact on human health. Zinc and iron concentrations in plant materials are commonly analyzed by flame atomic absorption spectrometry (F-AAS). This research describes the optimal analysis method for precise zinc (Zn) and iron (Fe) analysis of seeds from the seven species of genus Lens. Estimation of Zn and Fe concentration in whole lentil seeds by F-AAS is destructive, using nitric acid digestion of seeds prior to instrument analysis. The procedure proposed for the determination of Zn and Fe concentration uses the minimum amount of lentil seed of all lentil species with suitable standard reference materials. The relative standard deviations of the method were about 5% for both Zn and Fe. The minimum sample amount 0.3 g of wild and 0.5 g of cultivated lentil seed samples was identified for accurate and precise estimation of Zn and Fe concentration.     

Carbon and nutrient dynamics under long-term nutrient management in tropical rice-wheat-jute system

Impacts of 43-year nutrient management on carbon (C) and nutrient dynamics were studied in a rice-wheat-jute system in tropical India. Carbon dynamics was investigated on the basis of its distribution of labile (microbial biomass C, permanganate oxidizable C and water soluble C) and slow pools (Walkley Black oxidizable organic C), its indices (C pool index, lability index) and C management index (CMI) at different soil depths. Nutrient dynamics was judged by major nutrients availability, and related soil enzymatic activities. We studied seven treatments including unfertilized control, 100% NPK (N:P₂O₅:K₂O 60:30:60), 50% NPK, 150% NPK, 100% NP, 100% N, 100% NPK + farm yard manure (10 tonnes FYM ha^?1). Nutrients availability and soil enzymatic activities were significantly increased under balanced fertilization (100% NPK + FYM) over other treatments. Labile, slow and total C was also found highest in 100% NPK + FYM. Enhanced C indices under 100% NPK + FYM over 100% NPK and other treatments signified the importance of long term balanced fertilization on soil C stabilization. Lability indexes were lower at sub-soil (30-45cm). In terms of favourable nutrient dynamics and CMI, balanced application of NPK plus organics were recommend for long term sustainability of rice-wheat-jute system in tropics.     

Sulfur accumulation in Vertisols due to continuous gypsum application for six years and its effect on yield and biochemical constituents of soybean (Glycine Max L. Merrill)

Application of S through inorganic sources is recommended to the crops in the cropping system in S deficient soils of India. However, transformations of S in the soil profile following continuous gypsum application are not known. The accumulation of S into different fractions due to application of a total of 180—540 kg S ha^—1 over a six‐year‐period in soybean — wheat cropping system and its effect on the yield and quality of soybean produce were investigated in a vertisol area. About 25—38% of the S added was retained in the 90 cm depth of profile. Significant changes in the total S content in soil layers were recorded wherein a total of 360 kg S ha^—1 or more S was applied. Maximum increase in total S content was recorded in upper layers. Soluble S accumulated more in the 30—75 cm depth; whereas, proportion of sorbed fraction of S increased with depth. Build up of S in the surface layer of the profile was mostly related to the increase in organic fraction. The S accumulation in lower layers of the profile was mostly due to increase in soluble and sorbed fractions of S. Such an increase of the S content in the soil caused an increase in seed yield, uptake of S as well as content of oil and S containing amino acids, cystine, and methionine in seed of soybean crop.     

Tillage and irrigation effects on crop yields and soil properties under the rice-wheat system in the Indian Himalayas

Conservation tillage systems generally improve soil organic C (SOC), plant available water capacity (PAWC), aggregation and soil water transmission. A field experiment was conducted for 4 years (2001-2002 to 2004-2005) to study tillage (conventional tillage (CT) and zero tillage (ZT)) systems. The selected irrigation treatments were at four levels (I₁: pre-sowing (PS), I₂: PS + active tillering (AT)/crown root initiation (CRI), I₃: PS + AT/CRI + panicle initiation (PI)/flowering (FL), and I₄: PS + AT/CRI + PI/FL + grain filling (GF)), applied at the critical growth stages on rice (Oryza sativa L.) and wheat (Triticum aestivum L.). Their effects on direct seeded rice productivity and soil properties (SOC and selected physical properties) after rice and wheat harvest were investigated. Soil organic C contents after rice and wheat harvest in the 0-15 cm soil depth were higher under ZT than under CT. Soil organic C increased significantly with I₂ over I₁ for both crops and with I₄ over I₂ for the wheat crop. The PAWC was significantly higher with ZT than CT. Zero tilled and frequently irrigated plots showed enhanced infiltration characteristics (infiltration rate, cumulative infiltration and sorptivity) and saturated hydraulic conductivity. Both direct seeded rice and wheat yields were not significantly different in the plots under ZT and CT. There was a significant increase in both rice and wheat yields in the plots under I₂ over I₁. However, water use efficiency between irrigation treatments was not significantly different. Hence, under direct seeded rice-wheat system in a sandy clay loam soil of the sub-temperate Indian Himalayas, farmers may adopt ZT with two irrigations in each crop for optimum resource conservation.     

Checking the pulse of the major commercial fisheries of lake Victoria Kenya,for sustainable management

The present study demonstrates the declining state of the major commercial fisheries of Lake Victoria, Kenya, a situation threatening sustainability of the lake's fishery. Data in the present study were derived from resource monitoring programmes that included hydro‐acoustics (2009–2018), trawl net fishing (2011–2018), frame surveys (2000–2016) and catch assessment surveys (2000–2015). The activities provided information on fish stocks and supported advice for fisheries management. The average fish stock densities for Tanzania, Uganda and Kenya of 8.92, 8.25 and 8.19 t/km², respectively, were relatively similar. Diplomatic and sustainable efforts for harmony in fish harvesting among the fishers of the riparian countries are encouraged given the interdependence of the lake. The Kenyan and River Kagera regions had a higher proportion (≈ 4% each) of big‐sized Nile perch (≥50 cm total length) in 2018, signifying the critical breeding areas for Nile perch. To sustain the fishery, there is need to enforce a 36%–44% effort reduction for all the major fisheries, and enforcement of gear limits to avoid harvesting of immature fish and destruction of the lake ecosystem.     

Role of irrigation and mulch on yield, evapotranspiration rate and water use pattern of tomato (Lycopersicon esculentum L.)

Irrigation management strategy invites the quantification of crop response to irrigation frequencies. Conventionally, mulches increase the yield and water use efficiency (WUE) to a great extent by augmenting the water status in the root zone profile. A field study was carried out during the winter season (November-March) of 2003-2004 and 2004-2005 at the Central Research Farm of Bidhan Chandra Krishi Viswavidyalaya (Latitude 22°58′N, Longitude 88°31′E and altitude 9.75 m amsl), Gayeshpur, India, to evaluate the effect of irrigation frequencies and mulches on evapotranspiration rate from tomato crop field as well as leaf area index (LAI), fruit yield and WUE of the crop. The experiment was laid out in a split-plot design where three irrigation treatments {rainfed (RF); CPE₅₀ and CPE₂₅ where irrigation was given at 50 and 25 mm of cumulative pan evaporation (CPE)} were kept in the main plots and the subplots contained four mulch managements {no mulch (NM), rice straw mulch (RSM), white polyethylene mulch (WPM) and black polyethylene mulch (BPM)}. Under CPE₂₅, tomato crop recorded significantly higher leaf area index (LAI) over CPE₅₀ and rainfed condition. LAI value under BPM was 9-30% more over other mulches. Maximum variation of LAI among different treatments was recorded at 60 days after transplanting (DAT). Fruit yield under CPE₂₅ was 39.4 Mg ha⁻¹; a reduction of 7 and 30% has been obtained under CPE₅₀ and RF condition. The use of mulch increased 23-57% yield in comparison to NM condition. Actual evapotranspiration rate (ET_R) was 1.82 mm day⁻¹ under CPE₂₅ and declined by 15 and 31% under CPE₅₀ and RF condition, respectively. The variation of ET_R among different mulches became more prominent under maximum water stressed (RF) condition, whereas the variation was negligible under CPE₂₅ frequency. Irrespective of mulching WUE was highest under moderately wet (CPE₅₀) soil environment. Among different mulches, BPM was responsible for attaining the highest WUE value (25.1 kg m⁻³), which declined by 22, 21 and 39% under WPM, RSM and NM, respectively.     

Use of RAPD-PCR for genetic analysis of Nicobari fowl of Andamans

1. The present study was conducted to estimate genetic relatedness among Nicobari fowls (Brown, Black and White) and an exotic bird (White Leghorn) using random amplified polymorphic DNA (RAPD) polymorphism. 2. A total of 25 decamer primers were screened among all the breeds of which 24 primers amplified the genomic DNA, generating 2000 to 200 bp bands. Ten primers generated reproducible and distinct RAPD profiles and were used for further analysis. 3. A total of 94 bands were amplified and 30 polymorphic bands (32%) were produced. The number of polymorphic loci ranged from 1 to 5 with an average of 3.0. 4. Among the native breeds Brown Nicobari showed higher genetic similarity (0.85) than Black Nicobari (0.80) and White Nicobari fowl (0.82). 5. Brown Nicobari showed high genetic similarity with Black Nicobari (0.87 +/- 0.029); least similarity was between White Nicobari and White Leghorn (0.77 +/- 0.028). 6. The RAPD profile of all Nicobari fowls on amplification with the primers PBG5 and PBA12 showed specific bands of molecular size 1050 and 785 bp, respectively. 7. The native breeds showed the least genetic distance with each other while White Leghorn appeared to be most distant from the native breeds.