Occurrence of vesicular-arbuscular mycorrhizae with Amaranthaceae in soils of the Indian semi-arid region

Summary Some members of the family Amaranthaceae, which has mostly been reported as non-mycorrhizal, were examined for a symbiotic association with mycorrhizae in the semiarid and arid zones. Ten species belonging to five genera, Achyranthes, Aerva, Alternanthera, Amaranthus, and Celosia were examined, using 1.0-cm long root standards. Intercellular hyphae, vesicles and arbuscules were observed in the root cortex. The number of different types of spores in the rhizosphere soil of different plants ranged from one to three. The spores isolated represented nine species belonging to four genera, Glomus, Gigaspora, Sclerocystis, and Scutellospora. No correlation could be established between spore counts and either soil pH or soil moisture.     

Lighting the way: Towards reducing misorientation of olive ridley hatchlings due to artificial lighting at Rushikulya, India

Sea-finding behavior in sea turtle hatchlings is modified by the visual cues provided by artificial beach front lighting. The consequent landward movement of hatchlings in response to coastal electric lighting reduces their survival rates. We assessed the potential impact of coastal lighting at Rushikulya, an important mass nesting site of the olive ridley sea turtle (Lepidochelys olivacea) in the Indian Ocean region. We examined the response of hatchlings to light characteristics in an experimental setup, as well as to the existing lighting regimes along the beach, using arena trials. Previous studies on other species indicate preferential orientation towards low wavelength and high intensity light. Our study confirms these preferences among hatchlings from the Indian Ocean population of olive ridleys. In addition we also found that wavelength and intensity could have an interactive effect upon hatchling orientation. Hatchlings at the study site respond both to visible point sources of light and to sheer glows of light. Though beach plantations of introduced Casuarina equisetifolia are generally considered to have negative impacts on sea turtle nesting beaches, we found that they acted as an effective light barrier when planted about 50 m away from the high tide line. We developed a model of the expected impact of artificial lighting on hatchling orientation during mass hatching events of previous years, and predict as much as 50% misorientation in some years. We also developed a map representing the misorientation of hatchlings due to artificial lighting based on arena trials in different regions of the beach. The results of the study helped identify focal areas for light management on the beach, which could be critical for the survival of this population.     

Germplasm innovation for high-temperature tolerance from traditional rice accessions of Kerala using genetic variability,genetic advance,path coefficient analysis and principal component analysis

Journal of Crop Science and Biotechnology - Ninety landraces of rice were evaluated for various physio-morphological traits in completely randomized design with two treatment levels, i.e. control...     

Pluchea lanceolata (Rasana): Chemical and biological potential of Rasayana herb used in traditional system of medicine

Pluchea lanceolata (DC.) Oliv. & Hiern, (Family: Asteraceae) is a rapidly spreading perennial herb, considered valuable for the management of anti-inflammatory disease. Scientific reports dealing with phytochemical and pharmacological research and its traditional have been reviewed. Reports have also suggested that its prominent constituents viz. triterpenoids, sterols, flavonoids and lactones originate from this plant arbitrate their effects by modulating several therapeutic targets.Out of about 80 species of Pluchea, some of them are on extinct and only 16 have traditional uses in several countries of Asian, Middle East and North American region. The present review covers the period 1935–2011.     

Preparation of nitrogen and phosphorus-enriched paddy straw compost and its effect on yield and nutrient uptake by wheat (Triticum aestivum L.)

Summary A nutrient-rich compost from paddy straw was prepared using urea and Mussoorie rock phosphate for N and P enrichment respectively. Inorganic N was partly conserved in the compost by the addition of pyrite. Citric-acid-soluble P also increased with the addition of pyrite. Compost containing about 1.6% total N and 3.3% total P was found to be a good source of P for a wheat crop and also supplied a significant amount of N to the plants.     

Clinical evaluation of detomidine-butorphanol-guaifenesin-ketamine as short term TIVA in Spiti ponies

Veterinarians working under remote field conditions are routinely presented with variety of surgical interventions in equines like castrations, management of wound, traumatic and congenital hernias and musculoskeletal disorders thus necessitating the use of general anaesthesia for management of these conditions. The present study was carried out to evaluate and recommend the suitable short term anaesthetic technique for Spiti ponies under field conditions. Seven clinically healthy male Spiti ponies presented for castration were evaluated for short term Total Intravenous Anaesthesia (TIVA) using detomidine (0.02 mg kg(-1)), butorphanol (0.01 mg kg(-1)), 5% guaifenesin (20 mg kg(-1)) and ketamine (2.0 mg kg(-1)). The studies conducted were open label trials and all the animals received same treatment. After proper tetanus prophylaxis and preanesthetic fasting, detomidine was administered intravenously. Subsequently at head down position the animals received butorphanol intravenously. Thereafter, guaifenesin was administered intravenously. As soon as the signs of ataxia developed, the induction of surgical anaesthesia was achieved by intravenous administration of ketamine hydrochloride. The onset of sedation was observed in 2.43 +/- 0.53 min following detomidine administration and the animals were ataxic in 1.43 +/- 0.43 min after butorphanol and guaifenesin administration when ketamine was injected. The ponies were in surgical plane of anaesthesia within 2.28 +/- 0.42 min following ketamine administration. During recovery the limb/head movement and sternal recumbency were attained in 18.71 +/- 1.98 and 26.14 +/- 1.62 min, respectively whereas standing ataxia and normal gait were seen at 29.42 +/- 3.21 and 71.14 +/- 4.74 min, respectively. There was excellent to good muscle relaxation. The surgical anaesthesia remained for 22.57 +/- 1.48 min. The recovery was smooth. Moderate to good suppression of palpebral and corneal reflexes were observed immediately after induction and during anaesthesia. The analgesia was excellent. A highly significant (p < 0.01) to significant (p < 0.05) decrease in respiration rate was observed after induction, during anaesthesia and after recovery. The mean SpO2 value in equines of this group was 76.50 +/- 4.14 and 83.33 +/- 4.18% after induction and during anaesthesia, respectively. Some of the blood biochemical parameters like plasma alanine amino transferase (ALT), total proteins and glucose showed significant increase without clinical consequence. It was concluded that detomidine (0.02 mg kg(-1)), butorphanol (0.01 mg kg(-1)), guaifenesin 5% (20 mg kg(-1)) and ketamine (2.0 mg kg(-1)) combination can safely be used for short term total intravenous anaesthesia in equines under field conditions where the monitoring facilities are meager.     

Variation and association analysis in coriander

Summary Two hundred genotypes of Coriandrum sativum L. exhibited genetic variation for plant height, primary and effective branches, days to flowering and maturity, umbels and umbellets per plant, grains per umbellet, thousand seed weight, straw yield and grain yield per plant and harvest index. Heritability estimates were high for days to flowering, thousand seed weight and days to maturity; moderate for plant height, straw yield, umbels per plant, umbellets per plant and number of primary branches; and low for harvest index, effective branches, grain yield per plant and grains per umbellet. Phenotypic correlations of grain yield per plant were highly significant and positive with umbellets per plant, umbels per plant, number of effective branches, straw yield per plant, number of primary branches, plant height, number of grains per umbellet and harvest index. Maximum direct contribution to grain yield per plant was made by umbellets per plant, followed by straw yield per plant, umbels per plant and grains per umbellet. Umbellets per plant made sizeable indirect effect via straw yield per plant. Straw yield per plant made sizeable indirect contribution via umbellets per plant.     

Bioactivity and structure–activity relationship of natural methoxylated phenylpropenes and their derivatives against Aphis craccivora Koch (Hemiptera: Aphididae)

In the light of recent increased interest in developing plant based secondary chemistry into products suitable for integrated pest management, the objective of the present study was to investigate the bioactivity in field simulated conditions and structure–activity relationship of some natural phenylpropenes and their related analogues (1–6) against Aphis craccivora. The activities of different compounds varied depending on the substitution of the functional groups and the side chain attached to the aromatic ring of the phenylpropene. Dimethyleugenol (5) showed maximum activity among all tested phenylpropenes with LC₅₀ values 880 and 2,047 ppm against nymphs and adults respectively. Present study showed that phenylpropenes appear to be promising natural insecticides and may hold potential for identification of new lead structures against A. craccivora. The activity of the most active phenylpropene (5) was comparable with that of the chemical pesticide (dimethoate).     

Amelioration of altered oxidant/antioxidant balance of Indian water buffaloes with subclinical mastitis by vitamins A, D3, E, and H supplementation

The effect of vitamins A, D₃, E, and H supplementation on oxidative stress indices in Indian water buffaloes suffering from subclinical mastitis was investigated. Changes in the total oxidant capacity (TOC), total antioxidant capacity (TAC), nitric oxide (NO), and activities of glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) in milk were evaluated before and after the supplementation of vitamins A, D₃, E, and H. The buffaloes suffering from subclinical mastitis revealed remarkable alterations in the milk oxidants/antioxidants balance shifted towards oxidative status. The buffaloes with subclinical mastitis revealed significantly (P?≤?0.01) higher TOC, NO contents, and CAT activity, while TAC content and GSH-Px activity were significantly (P?≤?0.01) lower in comparison with the healthy controls. However, SOD activity did not show any significant change. Supplementation of vitamins A, D₃, E, and H to these animals revealed significant (P?≤?0.01) reduction in TOC, NO, and CAT, while a significant (P?≤?0.01) increase in TAC and GSH-Px activity was also evident. From the present study, it may be concluded that supplementation of these vitamins can help ameliorate the altered milk oxidants/antioxidants balance towards normalcy and, thus, ensue recovery from subclinical mastitis in the Indian water buffaloes.     

Generation of superior germplasm of Jatropha curcas through ex vitro grafting

An efficient technology capable of cloning superior Jatropha curcas germplasm through grafting on large scale was standardized. This technique overcomes the bottlenecks associated with conventional seed and cutting raised plants. This is a novel technique being capable of establishing well-rooted pure strands of superior genotype utilising stock–scion concept and also explores possibilities for the improvement of existing inferior strands into high yielding and superior varieties. Among the various grafting methods tried, top cleft grafting was found to be most successful. The ideal stock–scion diameter and grafting height was found to be 3–4 cm and 40–60 cm, respectively. Top cleft grafting was more successful, stable and strong in comparison to other grafting methods, exhibiting 92.0 and 94.09 % success during spring and monsoon seasons, respectively. Side cleft grafting proved to be the second best method resulting in 80.0 and 82.23 % success during spring and monsoon seasons, respectively.