排序方式: 共有104条查询结果,搜索用时 15 毫秒
11.
12.
宣化庭院漏斗架式葡萄栽培模式的生态系统服务功能研究 总被引:1,自引:0,他引:1
宣化牛奶葡萄以庭院式栽培为主,目前仍大量沿用传统的漏斗式栽培模式。为了给这种传统葡萄栽培方式的传承和保护提供科学依据,采用野外观测及农户调查等方法对漏斗葡萄栽培模式的生态系统服务功能进行了研究。研究结果表明,该模式葡萄产量为2.65万kg/hm2,经济收益可达18.38万元/hm2,宣化区总产量达到3 600多t,经济收入达到2 500万元;该栽培模式在庭院中创造了丰富的生态位,具有丰富的生物多样性,在调查的30个农户中,观察到87种植物,分属43科62属;漏斗葡萄地上部分碳贮量可以达到5.85t/hm2,而且由于寿命较长,葡萄植物体内存贮的碳能够得到长期固定;同时,漏斗葡萄造型优美,景观独特,具有突出的旅游开发价值。 相似文献
13.
对硝酸钙泥、硝酸镁泥两种化工废渣的磷吸附特性进行了研究。结果表明:用Langmuir方程能很好地拟合300℃焙烧后的硝酸钙泥硝酸镁泥和500℃焙烧后的硝酸钙泥硝酸镁泥对磷的等温吸附。300℃、500℃焙烧后的硝酸钙泥、硝酸镁泥的磷最大吸附量分别为37.45、32.36、35.59、28.82mg/g ,表面吸附强度因子分别为1.5615、0.2946、0.2948、0.2882mL/g ,最大缓冲能力分别为58.48、9.53、10.49、8.31mL/g。对磷的吸附效果最好的是300℃焙烧后的硝酸钙泥。300℃、500℃焙烧后硝酸钙泥、硝酸镁泥对磷的去除率达到83%~99%左右。去除率最好的是300℃的硝酸钙泥。 相似文献
14.
15.
本研究利用化学合成法合成了包含pCAMBIA0390左右边界T-DNA和LoxP/FRT(LF)位点的DNA片段Ⅰ,利用SacⅡ和SphⅠ酶切位点,去除了pCAMBIA0390左右边界T—DNA和多克隆位点之间的序列,然后连接DNA片段Ⅰ和载体片段,构建了植物表达载体pGM323-LF-enTP。随后,再合成含有适合在单予叶植物中表达的由玉米Ubi-1启动子驱动的融合标记基因(Bar::gus)和水稻actin-1启动子驱动的助抗虫蛋白基因(Cry1A6)表达元件的DNA片段Ⅳ,在pGM323~LF—enTP的基础上,利用5以Ⅰ和PstⅠ位点构建了同时含有LF位点、Bar::gus以及Cry1Ab基因表达元件的表达载体pGM626-LF—ABt。利用含有pGM626-LF—ABt的农杆菌遗传转化烟草和玉米,以草丁膦作为抗性筛选剂,非转化细胞得到了有效抑制,快速获得了转基W植株,利用GUS组织化学检测和RT—PCR分析了转基因植株中标记基因的表达,结果表明pGM626-LF—ABt可以用于农杆菌介导的单、双子叶植物遗传转化。本研究为培育安全抗虫转基因植物奠定了基础。 相似文献
16.
17.
18.
Background: Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results. The purpose of this study was to identify the most stable housekeeping genes in porcine articular cartilage subjected to a mechanical injury from a panel of 10 candidate genes. Results: Ten candidate housekeeping genes were evaluated in three different treatment groups of mechanically impacted porcine articular cartilage. The genes evaluated were: beta actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hydroxymethylbilane synthase, hypoxanthine phosphoribosyl transferase, peptidylprolyl isomerase A (cyclophilin A), ribosomal protein L4, succinate dehydrogenase flavoprotein subunit A, TATA box binding protein, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein-zeta polypeptide. The stability of the genes was measured using geNorm, BestKeeper, and NormFinder software. The four most stable genes measured via geNorm were (most to least stable) succinate dehydrogenase flavoprotein, subunit A, peptidylprolyl isomerase A, glyceraldehyde-3-phosphate dehydrogenase, beta actin; the four most stable genes measured via BestKeeper were glyceraldehyde-3-phosphate dehydrogenase, peptidylprolyl isomerase A, beta actin, succinate dehydrogenase flavoprotein, subunit A; and the four most stable genes measured via NormFinder were peptidylprolyl isomerase A, succinate dehydrogenase flavoprotein, subunit A, glyceraldehyde-3-phosphate dehydrogenase, beta actin. Conclusions: BestKeeper, geNorm, and NormFinder all generated similar results for the most stable genes in porcine articular cartilage. The use of these appropriate reference genes will facilitate accurate gene expression studies of porcine articular cartilage and suggest appropriate housekeeping genes for articular cartilage studies in other species. 相似文献
19.
20.