一种棉花DNA快速提取方法

本研究以棉花子叶为材料,探索一种适用棉花批量SSR-PCR分析的DNA快速提取方法。本方法只需要两次加液,两次高温水煮即可完成。紫外检测结果表明,所得DNA浓度较好,纯度较差,含有较多蛋白质。但SSR分析结果显示,可以扩增出相应的带型,且清晰易辨,准确可靠。相比常规CTAB法,具有操作简便,成本低,效率高,且无毒,无污染的优点,适用于大量棉花样品的SSR-PCR分析。     

几种药剂对苗木中星天牛幼虫毒杀效果初探

试验选用25 g/L溴氰菊酯、40%氧乐果乳油、啶虫脒、40%辛硫磷、80%敌敌畏乳油、茶皂素、10%吡虫啉可湿性粉剂、45%杀螟硫磷等药剂对星天牛幼虫进行浸泡处理,并研究了部分药剂的混配浸泡效果,得出结果:溴氰菊酯500倍、溴氰菊酯500倍+啶虫脒500倍、辛硫磷500倍+敌敌畏500倍处理星天牛幼虫15 min,4d后幼虫死亡率达到100%。在对红枫嫁接苗中星天牛幼虫的药剂浸泡试验中,溴氰菊酯500倍处理在浸泡48 h、辛硫磷500倍+敌敌畏500倍处理在浸泡36 h都可完全杀死星天牛幼虫。     

蜜蜂幼虫芽孢杆菌微滴式数字PCR检测方法的建立

为建立新型高灵敏度的幼虫芽孢杆菌微滴式数字PCR (droplet digital PCR,ddPCR)检测方法,针对幼虫芽孢杆菌16S rRNA基因设计特异性引物和探针。通过对反应体系优化,建立幼虫芽孢杆菌ddPCR检测方法,并对方法的灵敏度、线性关系、特异性及重复性进行评估。结果:建立的方法与其他6株非幼虫芽孢杆菌无交叉反应,具有良好的特异性;线性关系良好(R^2=0. 991 4);灵敏度为2. 9 copies/μL; ddPCR检测的相对标准偏差(RSD)在0. 93%～5. 27%,重复性好。试验表明本研究建立的ddPCR方法可以对幼虫芽孢杆菌进行绝对定量检测。     

加工型大蒜无公害高产栽培技术

大蒜中含有的硫化丙烯,也就是大蒜素(Allicin),具有强烈的杀菌作用.作为保健食品,具有行气消积、杀虫解毒、杀菌止痢、消痛及健胃等功效,因此,大蒜深加工产品在国际市场上非常畅销.     

广东佛山市试点征地制度改革 量化征地补偿标准

作为全国征地制度改革试点城市,广东省佛山市近年来通过规范统一的征地补偿标准,建立农民容易接受的征地程序,通过多元化的补偿方式,解决被征地农民后顾之忧,从而实现了和谐征地,既减少了征地纠纷,又为佛山经济发展提供了充足的空间。     

南非金盏草

南非金盏草(Arctotheca calendula)是一种入侵性和适生性极强的有害植物,传入澳大利亚后大面积扩散,并对农牧业生产造成严重影响。美国农业部也将其列入联邦有害杂草名单。本文从形态、生物学、传播途径、经济影响等多方面对其进行描述,提出应加强对澳大利亚进口农产品的检疫和监管力度。     

进境意大利苹果苗上葡萄茎枯病菌的截获鉴定

The isolate ZM40-1 was isolated on Mallus sp. imported from Italy in Ningbo port traditional PDA method. The isolate grew well on three kind of medium, including PDA, MEA and OA, producing mostly little aerial mycelium and abunt pycnidia. The pycnidia were globose or subglobose, 36.4-191.8(87.2) μm in diameter and conidia were variable in shape, mostly ovoid-ellipsoidal, single cell, sometimes curved, 3.3-6.2(5.1)μm ×1.9-3.1(2.7) μm in diameter and hyaline. Chlamydospores were mostly multicellular, solitary or in chains, brown and 18.0-48.0(35.2) μm×7.4-18.7(13.8) μm in size. To compare the isolate sequences (ITS, 28S, ACT and TUB) with the identity of respestive sequence of Phoma glomerata from NCBI GenBank databases by blastn method, the isolate DNA was amplified and sequenced by universal primers. Analysis of these sequences with the related sequences deposited in the NCBI database showed that these sequence had 100% identities, respectively. Pathogenicity tests showed that the isolate didn’t infect stem of Mallus sp.. However, the fruit and leaf were susceptible to P. glomerata. Based on morphological characteristics, molecular and pathogenicity test results, the isolate ZM40-1 was identified as Phoma glomerata (Corda) Wollenweber & Hochapfel. To our know-ledge, this is the first report of interception and identification of P. glomerata in China.     

应用酯酶同工酶测定秦油2号种子纯度

应用酯酶同工酶测定秦油２号种子纯度陈先锋，陈龙英，顾万昌，许国慰安徽省合肥市种子公司近年来，随着杂交油菜秦油２号在我省种植面积的不断扩大，杂交油菜种子的用量逐年增加。为了给生产上提供高纯度的杂交一代油菜种，保证其增产优势，杜绝伪劣种子在市场上销售，我...