烟草线条病毒RT-PCR检测方法

根据已报道的烟草线条病毒外壳蛋白基因序列，设计出一对特异性引物，提取大豆病叶的总RNA，反转录成cDNA，PCR扩增出约400bp大小的产物，产物克隆到pGEM-T Easy载体上，转化大肠杆菌DH 5α，抽提的质粒用Not Ⅰ酶切，出现430bp大小的目标插入片段。经序列测定确认了插入片段的大小为404bp。     

德国核桃‘No.120'幼胚胚轴与子叶体细胞胚胎发生及其植株再生

核桃 (Juglansregia)‘No . 12 0’品系授粉 7～ 8周的幼胚胚轴与子叶在含不同植物生长调节剂组合的诱导培养基上培养 4周后转移至无植物生长调节剂的继代培养基上培养 ,不同发育时期的体细胞胚直接形成于胚轴与子叶表面。子叶与胚轴分别在KT 2mg/L BA1mg/L IBA 0 .0 1mg/L与 2 ,4 D 2mg/L KT 1mg/L的组合的诱导下体细胞胚胎发生频率最高。体细胞胚经 3～ 5℃低温处理 2个月 ,31.6%的体细胞胚能发育成正常生长的植株     

荔枝果皮的褐变

荔枝果皮的褐变一直是商业上的一个主要采后问题。大多数人认为褐变是由于果实失水后多酚氧化酶(PPO)活性增加引起的,它导致了花青苷的迅速降解,使果变褐。然而,支持此理论的实验证据很少。最近,澳大利亚和以色列着重研究果皮液泡的PH值对花青苷颜色和稳定性的影响。本文综述了研究荔枝果皮褐变的方法,并针对目前流行的PPO引起花青苷降解的理论提出一些疑问。     

俄罗斯里海北外围平原半荒漠地区沙棘的生态特征

本文对生长在俄罗斯里海北外围平原半荒漠地区两种不同生境下的沙棘适应性进行了研究。此项研究在俄罗斯科学院的森林科学研究所Dzhanybek实验研究站进行。该实验站位于伏尔加河和乌拉尔河之间的里海低地（290万hm^2）的粘土半沙漠区,自然条件具有代表性。     

Introduction of Bacillus thuringiensis(Bt) gene does not reduce potassium use efficiency of Bt transgenic cotton(Gossypium hirsutum L.)

Background: Potassium(K) deficiency has become a common field production problem following the widespread adoption of Bacillus thuringiensis(Bt) transgenic cotton(Gossypium hirsutum L.) worldwide. The purpose of this study was to clarify whether the introduction of Bt gene directly reduces the K-use e ciency of cotton to induce K deficiency.Results: The cotton variety, Jihe 321(wild type, WT) and its two Bt(Cry1Ac)-transgenic overexpression lines(OE-29317, OE-29312) were studied in field with lo...     

Cotton stomatal closure under varying temperature and vapor pressure deficit,correlation with the hydraulic conductance trait

Background: Cotton(Gossypium hirsutum L.) is often grown in locations characterized by high atmospheric evaporative demand. It has been hypothesized that plants which resist hydraulic flow under this condition will limit water use and conserve soil water. Therefore, in a series of controlled environment experiments ten cotton cultivars were exposed to two di erent temperature and vapor pressure deficit(VPD) conditions(i.e., 38 °C, > 3 kPa and 32 °C,1～1.5 kPa) as well as a progressive soil dry...     

植物-土壤微生物反馈在草地演替过程中的作用机制

植物-土壤反馈影响生态系统结构、功能及过程,推动植物群落组成的变化,对解释植物群落的演替进程和方向、维持植物群落多样性和稳定性有着重要意义,已成为近年生物学和生态学研究热点。植物-土壤微生物反馈是植物-土壤反馈的重要组成部分。重点综述植物-土壤微生物反馈在植物群落演替过程中的作用机制,简要指出目前研究中存在的不足,并对未来研究中值得重点关注的科学问题进行了探讨与展望。     

Valuing Potential Benefits of Research into the Biological Control of Californian Thistle （Cirsium arvense）： A Bioeconomic Model

The present research developed a bioeconomic model for control of Californian thistle （Cirsium arvense L. Scop.） in intensive, lowland sheep pastures in New Zealand. Production costs included two control methods： herbicides and defoliation, with defoliation encompassing both physical means and biocontrol measures. The model was used to examine the economic value of a research programme into biological control of the weed, given several different future scenarios. These scenarios were high herbicide prices, a price premium for lamb raised without chemical herbicides, and development of herbicide resistance. The model results were used to estimate national impacts. If the only change in the agricultural sector was the cost of the biocontrol to the nation＇s farmers, the research programme to find an effective biocontrol agent had little economic impact. If other factors changed, such as the price of petroleum or levels of herbicide resistance in the weed. the model＇s results suggested that intensive sheep farming could grow or shrink by as much as NZ$219 million dollars over five years. In the latter case, having an alternative control method reduced potential losses and increased potential gains, resulting in a net benefit of between NZ$81 million and NZ$153 million. This work extended prior research in several ways. First. Californian thistle reproduces via root buds, which affected the modelling of weed behaviour. Secondly, the model allowed optimistation over continuous levels of weed control for two different methods. Thirdly, the model accounted for both weed control and damage to nitrogen-fixing clover from herbicide use. Finally, the research investigated optimal weed control in several different alternative future states.     

Evaluation of Some Physical Properties of Cucumber （Cucumis sativus L.）

Cucumber is one of the important vegetables in many countries. Information about physical properties of cucumber is necessary for designing, grading, sorting and processing operations. In this study, some physical properties such as diameter, length, height, and weight of three varieties of cucumber （Green Gold, Dharwad, and Super Dominus） were measured. The results revealed that there was no significant difference （P〈0.01） in the density values of the studied varieties, but regarding other physical properties there were a significant difference （P〈0.01） among them. The average density for Green Gold, Dharwad and Super Dominus were 0.98, 0.95 and 0.94 g/cm^3, respectively. Green Gold cucumber had the highest diameter, volume, weight, flesh diameter, geometric mean diameter and sphericity. Dharwad and Super Dominus varieties with 15.49 cm length and 4.61 length to diameter ratio had the highest length and length to diameter ratio, respectively. Smallest skin thickness （1.48 cm） was for Super Dominus. Surface area of Green Gold, Dharwad and Super Dominus were 192.29, 192.4 and 131.2 cm^2. As well as there was a high and positive correlation between weight and volume in the studied varieties. There was a non-significant and low correlation （0.56） between diameter and length in Super Dominus. There was also a high and positive correlation between diameter and geometric mean diameter in all the varieties.     

Effects of Gradual Water Deficit Stress on Phenological and Morphological Traits in Chickpea （Cicer arietinum L.）

The aims of this study were to investigate the effect of gradual water deficit stress on some phonological and morphological traits and grain yield of desi and kabuli chickpea cultivars. This study was carried out in 2007 and 2008, to evaluate responses of three chickpea cultivars （Hashem and Arman from kabuli and Pirooz from desi type） under well watering （I1： 70mm evaporation from class A pan）, gradual water deficit （12 and 13： 70→90→ 110→130 and 70→100→130mm evaporation from class A pan, respectively） and severe water stress （14： 130mm evaporation from class A pan）. Result showed that days to flowering and plant height were decreased, as water limitation increased. This reduction was significant under gradual water stress （I2 and I3） and Severe water deficit （I4）, compared with control （I1）. There were no significant differences in grain filling period and grain yield among I~, I2 and I3 irrigation treatments. No significant differences in days to physiologic maturity and number of sub branches were recorded among irrigation treatments. Interactions of year×cultivar for days to physiologic maturity, grain filling period and grain yield （P≤0.01） and for days to flowering and plant height （P≤0.05） were significant. The superiority of Arman in producing comparatively greater grain yield could be attributed to higher grain filling period of this cultivar in both years.