一种沼液冲厕装置

目前农村所建户用沼气池基本上都是厕所、猪圈、沼气池三结合,人畜粪便虽能自流入池,但厕所常有积便,每到夏季蝇蛆滋生,既不卫生,又易传播疾病,要保持厕所卫生就必须经常冲洗.     

Effect of bFGF on glucose regulated protein 78 expression and apoptosis in human ovarian cancer CAOV3 cells

AIM: To evaluate the effect of bFGF on the apoptosis and the expression of glucose regulated protein 78 (GRP78) in ovarian cancer CAOV3 cells.METHODS: Starvation induced cell apoptosis was conducted. After treatment with bFGF, the cell cycle and apoptosis were determined by FACS analysis and Annexin V/PI staining, respectively. The expression of protein kinase B (PKB) and GRP78 were detected by Western blotting and RT-PCR. RESULTS: Compared to starvation group, the cells treated with bFGF were still viable and increased activation of PKB and high expression of GRP78 were observed, which were prevented by PKB inhibitor wortmannin effectively (P<0.01). CONCLUSION: bFGF plays a critical role in anti-apoptosis and proliferation in human ovarian cancer through PKB signal transduction pathway, partly by upregulating the expression of GRP78.     

Preharvest applications of growth regulators and their effect on postharvest quality of table grapes during cold storage

Over 54,600 ha of table grapes (Vitis vinifera), mainly cvs. ‘Thompson Seedless’, ‘Flame Seedless’ and ‘Redglobe’, are planted in Chile. Almost the entire production is exported to the USA, Europe, Asia, or one of several Latin American countries, which typically requires 15–40 d of maritime transportation. During this period, several physical, physiological, and pathological factors cause table grape deterioration. Because berry size is the main quality factor in international markets, farmers often overuse the growth regulators, gibberellic acid (GA₃) and forchlorfenuron (CPPU), in an effort to increase berry size. We examined the effect of preharvest growth regulators on seedless (‘Thompson Seedless’, and ‘Ruby Seedless’) and seeded (‘Redglobe’) table grape cultivars during cold (0 °C) storage plus a shelf life period of 3 d at 20 °C. The overuse of GA₃, eight instead of two GA₃ applications on Thompson Seedless, and the use of one GA₃ application on Redglobe and ‘Ruby Seedless’, increased berry pedicel thickness and lowered cuticle content but induced shatter and predisposed grapes to gray mold caused by Botrytis cinerea. In contrast, CPPU increased berry pedicel thickness and cuticle content but did not increase shatter or gray mold incidence. Clusters that were subjected to overuse of combined GA₃ and CPPU were highly sensitive to shatter, had the thickest pedicel, and developed a high gray mold incidence during cold storage. Hairline, a fine cracking developed during cold storage, was induced on ‘Thompson Seedless’ and ‘Ruby Seedless’ by growth regulators, but no hairline occurred on ‘Redglobe’ table grapes. Therefore, berry quality during cold storage is greatly influenced by growth regulator management in the vineyard.     

Biological characteristics of long passaging rat mesenchymal stem cells

AIM:To investigate multi-potential of rat bone marrow mesenchymal stem cells (rBMMSC) and mutation inclination, the rBMMSC were long passaged in vitro. METHODS:Cellular cycles of different passages were assayed by FACSan flow cytometry and karyotypes of passage 6, passage 25 and passage 45 were compared by G-binding analysis. RESULTS:The early passages and long-term passages all showed strong proliferation; passage 6, passage 25 and passage 45 all showed normal karyotype. CONCLUSION:Long-term culture and passage of rBMMSC still remains strong proliferation. With this capability, the mutation inclination is not enhanced.     

Effects of changing PKC activity on proliferation and telomerase expression in human hepatocellular carcinoma cells

AIM: To investigate whether protein kinase C (PKC) is involved in the proliferation and the telomerase expression in human hepatocellular carcinoma cells. METHODS: Human hepatocellular carcinoma cells (BEL-7402) were treated with exogenous phorbol-12-myristate-13-acetate (PMA, PKC activator) and staurosporine (SP, PKC inhibitor) for 48 hours. The techniques of cell culture and the telomeric repeat amplification protocol silver staining in combination with computer image scanning system in vitro were used to observe the variations of the growth and the telomerase expression. RESULTS: The proliferative potential of BEL-7402 cells was decreased by the action of PMA as well as SP, and the telomerase expression was also inhibited by PMA and SP. CONCLUSION: Our findings suggest that the proliferation of human hepatocellular carcinoma cells and the telomerase expression may be related to PKC.     

Effects of fosinopril,captopril and valsartan on the expressionof tissue factor in human monocytes

AIM: To investigate the effects of angiotensin-converting enzyme inhibitors (ACEI), fosinopril, captopril and angiotensin II AT₁ antagonists, valsartan on tissue factor (TF) expression on monocytes induced by lipopolysaccharide (LPS). METHODS: Mononuclear leukocytes from normal delivered female umbilical veins were incubated with bacterial LPS in presence or absence of different ACE inhibitors .At the end of incubation, the cells were disrupted by 3 freeze-thaw cycles. TF procoagulant activity was assessed by a one-stage clotting assay. RT-PCR was used to check TF mRNA expression, and GAPDH mRNA was used for parallel assay. RESULTS and CONCLUSION: The results showed that increased expression of TF mRNA induced by LPS was inhibited by fosinopril, captopril and valsartan, respectively, and the procoagualant activity of monocytes was also reduced.     

氮、磷、钾肥不同施用量对马铃薯产量的影响

马铃薯种植面积在德江是仅次于水稻、玉米的第三大作物,常年种植面积在0.93万hm2左右.由于农户在种植马铃薯时普遍存在重氮轻磷缺钾的现象,导致马铃薯产量不高,经济效益低下.为了提高马铃薯产量,增加经济效益,特设置施肥对马铃薯产量的影响试验.     

云南省6县退耕还林农户收益分析及增收对策的案例研究

采取抽样调查的方式,对云南省6个地(市)退耕农户进行调研,对农户的林业收入状况、在退耕地所经营的林业项目以及退耕面积与收益等3方面进行深入剖析,认为农户的林业收入有高型、中高型、中等型、偏低型以及低下型5种类型.对退耕还林发展林业过程中面临的主要问题和促进当地林业发展、增加农户收入的对策进行了探讨.     

达乌里胡枝子花粉萌发特性研究

为揭示达乌里胡枝子(Lespedeza daurica)花粉的育性并为其花粉活力检测提供一种高效方法,本试验以达乌里胡枝子新鲜花粉为试验材料,采用液体培养基,研究了蔗糖、硼酸、氯化钙浓度、采样时间、温度、光照和培养时间等因素对花粉离体萌发和花粉管生长的影响.试验结果表明:单因素试验中,适宜花粉萌发的培养基配方为20 g...     

利用SSR标记分析高粱属种质资源的遗传多样性

本研究利用15对SSR 引物对161 份高粱属种质资源进行了遗传多样性分析。结果显示,15对引物共扩增出118条谱带,其中88条具有多态性,多态性比率(P)为75.21%;多态性信息含量(PIC值)变幅为0.612~0.806,平均为0.699。161份高粱属材料遗传相似性系数为0.636~0.977,平均基因多样性指数(H)为0.696,Shannon信息指数(I)为1.393。UPGMA聚类分析显示, 161 份高粱属材料在遗传相似系数(GS)为0.682处可分为3组;129份高粱和苏丹草材料在相似系数为0.671处可分为2组。表明SSR标记可以作为高粱属种间以及种内遗传分化分析的有力工具,被分析的高粱属种质材料具有较高的遗传多样性。