Antihypertensive Effects of IGTGIPGIW Peptide Purified from Hippocampus abdominalis: p-eNOS and p-AKT Stimulation in EA.hy926 Cells and Lowering of Blood Pressure in SHR Model

The aim of this study was to assess the potential hypertensive effects of the IGTGIPGIW peptide purified from Hippocampus abdominalis alcalase hydrolysate (HA) for application in the functional food industry. We investigated the antihypertensive effects of IGTGIPGIW in vitro by assessing nitric oxide production in EA.hy926 endothelial cells, which is a major factor affecting vasorelaxation. The potential vasorelaxation effect was evaluated using 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate, a fluorescent stain. IGTGIPGIW significantly increased the expression of endothelial-derived relaxing factors, including endothelial nitric oxide synthase and protein kinase B, in EA.hy926 cells. Furthermore, oral administration of IGTGIPGIW significantly lowered the systolic blood pressure (183.60 ± 1.34 mmHg) and rapidly recovered the diastolic blood pressure (143.50 ± 5.55 mmHg) in the spontaneously hypertensive rat model in vivo. Our results demonstrate the antihypertensive activity of the IGTGIPGIW peptide purified from H. abdominalis and indicate its suitability for application in the functional food industry.     

Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

The endometrium of sheep consists of plenty of raised intercaruncular areas （IC）. In order to better understand aglandular areas called caruncular （C）, and intensely glandular the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins （DEPs） （P 〈 0.05）, functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.     

滇中冷凉山区不同播期反季花椰菜产量效益分析

为摸索滇中高海拔冷凉山区反季节栽培花椰菜的最佳播期以集成高效栽培技术推广应用,于2017—2018年选择海拔2250 m的云南省峨山县塔甸镇大西村地块进行9个播期的2年随机区组试验。结果表明,花椰菜生育期随播期推迟而延长,而花球采收期除播期7月10日外随播期推迟而逐渐增长;花椰菜株高、外叶数、开展度、球高、球径和单球重等农艺性状有随播期延迟呈现先逐渐减小而后又逐渐增大的趋势;莲座期黑腐病和霜霉病的病情指数随着播期的延迟呈现先逐渐升高而后又逐渐下降的趋势;花椰菜小区产量随着播期的延迟呈现先逐渐下降而后又逐渐提高的趋势,播期4月20日和4月30日与其余7个播期产量之间的差异达极显著水平。综合花椰菜在冷凉山区反季节栽培的生产实际和各播期产量产值及商品性表现,推荐滇中高海拔冷凉山区反季节栽培花椰菜的最佳播期为4月20—30日。     

黄土高原坡改梯综合效益分析——以燕沟流域为例

 退耕坡地是近年来黄土高原整治生态环境和控制水土流失的关键措施,但对于如何退耕,学术界存在多种不同观点,实践方面存在多种不同模式。以延安燕沟流域坡改梯为例,利用2005年延安站的逐日气象数据和燕沟流域地貌、土壤及土地利用等资料,借助WIN-YIELD软件,对不同地形坡度条件下坡改梯在作物增产、保水减沙效益以及燕沟流域坡改梯实践效果进行模拟、对比和实证分析。结果表明:地形坡度越大,坡改梯作物增产、保水和减沙效益越显著;坡度小于5°的坡耕地可以不进行梯田改造,坡度大于15°的坡耕地应及早实施坡改梯或退耕还林(草)。     

比格犬的繁殖技术Ⅰ.采精及精液冷冻

采用手握法采取比格犬精液,成功率为71.4％,平均射精量2.67mL;精液呈弱酸性,每次射出的精液分三段,精子主要在第二段,精子平均密度为2.105×10~8/mL;新鲜精液的活力为0.8～0.9;精液用两种稀释液稀释后在液氮中保存14～60d,解冻后,其孵活率分别为0.425和0.427.     

Effects of Compound Plant Nutrients on Rumen Fermentation Parameters,Rumen Microbiota and Fatty Acid Composition of Longissimus dorsi Muscle in Finishing Small Tail Han Sheep北大核心CSCD

This study was conducted to investigate the effects of compound p1ant nutrients (CPN) on rumen fermentation parameters, rumen microbiota and fatty acid composition of longissimus dorsi musc1e in finishing sma11 tai1 Han sheep. Sixteen 4-month-o1d finishing sma11 tai1 Han sheep with an initia1 body weight (BW) of (24.18±0.31) kg were random1y divided into two groups, name1y, with 8 rep1icates per group and 1 sheep per rep1icate. The sheep in the contro1 group were fed a basa1 diet, whereas the sheep of the contro1 group (CPN group) was fed the basa1 diet supp1ementation with 3‰ CPN. The experiment 1asted for 97 days after 7 days adaption. The resu1ts showed as fo11ows: compared with the contro1 group, 1) adding CPN decreased the concentration of ammonia nitrogen (NH3-N) (P<0.05); 2) CPN supp1ementation affected beta diversity of rumen microbiota; 3) the re1ative abundance of Acidobacteriota, Erysipe1otrichaceae_UCG-002, Lactobacillus, and Megasphaera were enhanced by adding CPN (P<0.05), whereas, the re1ative abundance of Bacteroidetes and Prevotella was 1ower (P<0.05); 4) the supp1ementation of CPN had no significant effect on fatty acid composition of longissimus dorsi musc1e of fattening sma11-tai1ed Han sheep (P>0.05); 5) the contents of tota1 MUFA, C18:1n9c, C14:1, C16:1, C18:2n6c, C18:3n3 and n-3PUFA in the longissimus dorsi musc1e were corre1ated with the re1ative abundance of Megasphaera, Erysipe1otrichaceae_UCG-002, Succiniclasticum and Ruminococcus (P<0.05). In conc1usion, CPN can regu1ate the rumen microbiota structure and reduce the rumen NH3-N concentration of fattening sma11-tai1ed Han sheep. In production practice, CPN can be used as a rumen eco1ogica1 regu1ator. © 2023 Authors. All rights reserved.     

Foot-and-mouth disease eradication efforts in the Republic of Korea

On March 20, 2000, a suspected vesicular disease in cattle was reported to the National Veterinary Research and Quarantine Service (NVRQS) of the Republic of Korea. This represented the index case of a foot-and-mouth disease (FMD) outbreak, which spread through several provinces. The Republic of Korea had been free of FMD for 66 years prior to the reintroduction of the virus and had recently suspended imports of pork and pork products from neighboring Japan owing to a reported FMD outbreak in that country. The Korean outbreak was ultimately controlled through the combination of preemptive slaughter, animal movement restrictions, and a strategy of ring vaccination. The purpose of this paper is to review the current FMD situation in Korea in the aftermath of its 2000 epizootic and how it may affect future efforts to eradicate or reduce risk of reintroduction of the disease into Korea.     

浅议非主要农作物品种不审定制度及其品种保护

论述了非主要农作物品种的管理及其保护是种子管理中不可忽缺的组成部分,指出非主要农作物品种不审定制度给种业市场带来的问题,对非主要农作物品种侵权现象进行了分析,并对完善非主要农作物品种不审定制度及其品种保护提出对策措施。     

低温环境下饲粮电解质平衡对后备蛋鸭激素分泌及营养物质代谢的影响

本试验旨在研究低温环境下饲粮电解质平衡(DEB)对后备蛋鸭激素分泌及营养物质代谢的影响.试验采用11周龄金定蛋鸭600只,随机分成5组(每组20个重复,每个重复6只试鸭),DEB值分别为144.18(Ⅰ组)、194.94(Ⅱ组)、247.14(Ⅲ组)、299.34(Ⅳ组)和351.54 mmol/kg(Ⅴ组).试验期为7周,环境温度为(1±1) ℃.结果表明:Ⅲ组血清中四碘甲腺原氨酸含量和四碘甲腺原氨酸/三碘甲腺原氨酸显著高于Ⅰ组(P＜0.05),但与Ⅱ组差异不显著(P>0.05);Ⅱ组血清中胰岛素含量显著高于Ⅴ组(P＜0.05),但与Ⅲ组差异不显著(P>0.05);血清中三碘甲腺原氨酸和皮质醇含量各组间差异不显著(P>0.05).DEB对血清中卵泡刺激素、黄体生成素、雌二醇含量影响显著(P＜0.05),且最高值均出现在Ⅱ组;对血清中孕酮含量影响不显著(P>0.05).随着DEB值的升高,血清中游离脂肪酸含量呈先上升后下降的趋势,Ⅱ组显著高于Ⅰ、Ⅳ和Ⅴ组(P＜0.05);血清中尿素氮含量呈先下降后上升的趋势,Ⅱ和Ⅲ组显著低于Ⅴ组(P＜0.05);血清中葡萄糖含量呈先上升后下降的趋势,Ⅱ和Ⅲ组显著高于Ⅳ和Ⅴ组(P＜0.05).由此得出,在低温环境下,当DEB值为194.94～247.14 mmol/kg时可促进后备蛋鸭激素分泌及营养物质代谢.     

Genetic Diversity and Population Structure in the Heavily Exploited Korean Rockfish,Sebastes schlegeli,in Korea

The Korean rockfish, Sebastes schlegeli, is a valuable and intensively exploited species in Korea. We discuss the genetic diversity and genetic structure of four Korean rockfish populations using eight microsatellite loci. In total, 161 different alleles from 138 individuals were observed. Average allele number per locus ranged from 2.5 to 23 and allelic richness varied from 13.38 to 14.63 within a population. Despite a long history of stocking practices, we found very high levels of polymorphism (mean heterozygosity = 0.810), which is comparable to other congeneric species. No significant difference in genetic diversity and molecular genetic variance (F_ST and R_ST) was observed among four local samples (P > 0.1). Little indication of contemporary inbreeding (F_IS= 0.051) or population structure (K = 1) was detected. This absence of differentiation may reflect high levels of gene flow along the coast of Korea. Our study demonstrates that rockfish in Korea should be managed as a single unit. Currently, the species does not appear to be genetically threatened, but the potential for a rapid loss of genetic diversity remains. This information on the genetic characteristics of Korean rockfish populations has important implications for fisheries management and conservation efforts, and will aid in the sustainable exploitation of the fishing resources and the preservation of biodiversity.