Effects of psychological stress on in vitro expression of activated surface molecules on T cells of peripheral blood from healthy persons

AIM: To study cellular and molecular mechanism involved in increasing susceptibility of infection in psychological stress persons. METHODS: Comparative studies were performed with double staining and flow cytometry analysis on immunophenotyping and in vitro expression of early activating surface molecule CD69 in response to mitogens on T cells from peripheral blood of 20 healthy college student volunteers before and after psychological stress. A series of term final examinations was defined as psychological stress. RESULTS: Immunophenotyping analysis showed no statistically significant difference in the percentage of CD2, CD3, CD4, CD8, CD19, CD20, CD16 and CD56 positive lymphocyte populations before and after psychological stress. There was a statistically significant decrease in the in vitro expression of CD69 in response to polyclonal stimulators on the T cells from persons after psychological stress than those before psychological stress. The percentage of CD69 expression (CD69⁺CD3⁺/CD3⁺%) in response to PHA and PDB in the whole blood culture for 72 hours decreased respectively from 28.1±4.1 and 80.7±6.8 on the T cells obtained before psychological stress to 17.6±3.8 and 65.8±7.9 on those obtained after psychological stress, while there was no statistically significant difference between the CD69 expression rates without stimulators on the T cells obtained before and after psychological stress. CONCLUSIONS: The effects of psychological stress to immune system is not on the level of changing proportions of the sub-populations within peripheral blood lymphocytes. Psychological stress can decrease the activating response of T cells in healthy persons, which may be responsible for the increase of susceptibility to infection in the psychological stress persons.     

CYP450 epoxygenase/EET pathway attenuates adipose inflammation of obese mice through HIF-1α

AIM To investigate the effects of cytochrome P450 （CYP450） epoxygenase/epoxyeicosatrienoic acid （EET） pathway on insulin resistance in obese mice, and to explore the possible mechanisms. METHODS High-fat diet-induced obesity model was established in C57BL/6Cnc mice, and the obese mice were randomly divided into 3 groups, including obesity group （treated with saline; n=10）, EET group （treated with 11,12-EET; n=10） and EET inhibitor 14,15-epoxyeicosa-5（Z）-enoic acid （EEZE） group （n=10）. Normal C57BL/6Cnc mice （n=10） treated with saline served as control. Protein expression of CYP2J2 （one of CYP450 epoxygenases） and hypoxia-inducible factor-1α （HIF-1α） was measured by Western blot. Vessel-like structure was detected by immunofluorescence staining. The serum levels of insulin, tumor necrosis factor-α （TNF-α）, interleukin （IL）-1β, IL-6 and monocyte chemoattractant protein-1 （MCP-1） were measured by ELISA. RESULTS In obese mice, homeostasis model assessment of insulin resistance （HOMA-IR） values were increased, the protein level of CYP2J2 was reduced, and the protein level of HIF-1α was increased in adipose tissues as compared with the controls （P<0.05）. The serum levels of MCP-1, IL-1β, IL-6 and TNF-α were also significantly increased in obese mice （P<0.05）. After treatment with 11, 12-EET, the HOMA-IR values were decreased compared with vehicle-treated obese mice, HIF-1α expression levels were decreased in the adipose tissue, and the serum levels of MCP-1, IL-1β, IL-6 and TNF-α were reduced （P<0.05）. Immunohistochemical results of adipose tissue from vehicle-treated obese mice showed a marked decrease in vessel-like structures （CD31-positive） compared with normal control mice （P<0.05）. EET treatment significantly increased the newly formed vessel-like structures in the visceral adipose tissues of obese mice as compared with vehicle-treated obese mice （P<0.05）. CONCLUSION High-fat diet-induced obesity and insulin resistance are closely related to the CYP450 pathway. Exogenous EETs effectively decrease obesity-induced insulin resistance possibly through pro-angiogenesis and attenuation of hypoxia and inflammation.     

岱海表层沉积物中氮素的水平分布及环境意义

针对岱海湖泊富营养化日趋严重及其水域面积逐渐萎缩的现实,本文系统地研究了TN、NH4^＋-N、NO3^--N和Org—N在岱海表层沉积物中的空间分布规律及其环境意义。结果表明,岱海表层沉积物中氮素的水平分布格局均体现出西北部、北部和东北部为高值区的特征,揭示出岱海的氮污染源主要分布于北岸和西北岸;岱海表层沉积物中氮的主要存在形式为有机氮,铵氮则为无机氮的主要存在形态;表层沉积物中全氮对底栖生物已经具有最低级别的生态毒性效应,对底栖生物群落及生态环境构成了威胁。     

间作对咖啡和澳洲坚果根系形态、分布及养分累积的影响

为探明澳洲坚果与咖啡间作对地上部生长及地下部互作效应,为生产推广复合高效栽培模式提供理论依据,布置了咖啡和澳洲坚果间作盆栽实验,以二者分别单作为对照,分析植株的生长量、生物量、养分含量、根系形态以及土壤养分的差异.结果表明:(1)间作咖啡和间作澳洲坚果根干重、茎干重、单株总干重分别为40.86、50.38、148.34...     

不同施肥处理对镉污染土壤雪里蕻光合特性的影响

通过盆栽试验研究了Cd污染土壤不同施肥处理对雪里蕻光合特性的影响,以期通过有效的农业措施降低土壤重金属污染对人类健康的影响.结果表明:不同施肥处理显著提高了雪里蕻的叶绿素含量和对光照强度的适应范围,使净光合速率(Pn)、光饱和点(LSP)和表观量子效率(AQY)显著升高,使光补偿点(LCP)降低.施用有机肥可以保持较高的Pn、LSP和AQY,而且与有机肥的施用方式有关.增施磷肥使雪里蕻的Pn和LSP最大.各施肥处理下Fv/Fm的值均大于0.8,表明雪里蕻未受到光抑制.可见,适宜的施肥措施可以改善Cd污染土壤雪里蕻的光合特性,从而促进其生长.     

现代生物技术在鲑鳟鱼类品种遗传改良中的研究进展

自从1970以来,渔业作为提供动物性食品增长最快速的行业,平均每年增长8.9%。自1950年到1990年间渔业捕捞量增加了5倍,但在最近15年间增长缓慢甚至出现停滞,而世界水产品消费则从1961年的280万吨增长到2001年的963万吨,增长了3倍之多,预计到2015年全球渔业产品的     

推广BMFB-4型高效免耕施肥播种机——提高保护性耕作机械化水平

我国农业生产环境脆弱,水资源紧缺,现有耕地94970khm^2,旱区约占60％,主要分布在日照长、温差大、富有农业生产潜力的北方和中西部地区。传统的耕作方式使土壤由于过度耕作而造成地表裸露,耕地退化,生态环境恶化。特别是近年来北方尘暴、沙化愈来愈烈,极大制约了农业的发展。迫切需要大力推广应用保护性耕作技术。对农田实行免耕少耕、残茬覆盖、生物覆盖、尽可能减少土壤耕作,减少土壤风蚀、水蚀,提高土壤肥力和抗旱能力。     

三系杂交粳稻新组合津粳优2018

津粳优2018是天津市国瑞谷物科技发展有限公司以优良BT型三系不育系津20A为母本,优良恢复系津恢18号为父本配组育成的杂交粳稻新组合,2017年通过国家农作物品种审定委员会审定,适宜在河南沿黄河及信阳地区、山东南部、江苏淮河以北、安徽沿淮河及淮河以北地区种植.     

小麦重要农艺性状QTL研究方法及研究进展

小麦的许多重要农艺性状都是数量性状,研究小麦数量性状遗传对小麦育种及其杂种优势具有十分重要的意义。近年来,分子生物学特别是分子标记技术的飞速发展,使得我们可以更深入地进行数量性状的研究。本文综述了近年来小麦QTL的研究进展,包括QTL定位原理及前提条件,常用的分子标记,QTL定位常用的作图群体,QTL定位的方法,QTL定位的统计软件和阈值,QTL的作图精度及小麦QTL定位的研究进展。讨论了小麦QTL定位存在的问题,展望了该领域的发展前景。     

防治长柔毛野豌豆生草果园杂草茎叶处理剂筛选

【目的】筛选出有效防治果园长柔毛野豌豆生草田杂草的茎叶处理剂,为果园长柔毛野豌豆生草田杂草的化学防治提供参考。【方法】室内盆栽比较了8种除草剂对长柔毛野豌豆的安全性和常见杂草的生物活性,筛选药剂进行复配,进一步研究复配制剂的联合除草类型,测定其对长柔毛野豌豆的安全性及其在长柔毛野豌豆与杂草之间的选择性指数,并对复配制剂进行田间药效试验。【结果】灭草松、咪唑乙烟酸与精喹禾灵茎叶喷雾处理在2×田间推荐剂量下对长柔毛野豌豆安全。咪唑乙烟酸在长柔毛野豌豆与碎米莎草、马唐、牛筋草、狗尾草、播娘蒿、牛繁缕之间的选择性指数大于2.0;灭草松在长柔毛野豌豆与荠菜、播娘蒿、牛繁缕之间的选择性指数小于0.7,安全性差;精喹禾灵在长柔毛野豌豆与牛筋草、狗尾草、马唐之间的选择性指数大于8.8。联合作用测定结果表明咪唑乙烟酸与精喹禾灵按不同质量比复配后对马唐、牛筋草、看麦娘、牛繁缕、播娘蒿、荠菜的联合作用类型均为加成作用,其中以质量比2∶1复配后,选择性指数在2.4～8.9之间。田间试验结果显示：咪唑乙烟酸和精喹禾灵复配后在有效成分125～150 g·hm^-2剂量下,于杂草2～3叶期、长柔...