香料烟育苗技术

介绍云南保山香料烟区根据试验和生产实践总结出的,包括苗床选地、苗床制作、播种、管理和病虫(?)害防治的一套香料烟育苗技术。     

南岳风景名胜区旅游环境容量浅探

随着旅游业的不断升温,景区资源保护与旅游开发利用之间的矛盾日益加剧,旅游环境容量问题也越来越受到人们的关注.以“五岳独秀”的南岳衡山为个案,通过对南岳风景名胜区旅游环境容量的测算以及现状游客量的调查并进行对比分析,揭示该风景区的旅游潜力;提出景区资源保护和旅游调控对策,在维持风景名胜区生态平衡、保证旅游质量的前提下,有计划地开发旅游资源,实现旅游业的可持续发展.     

Differences in Response of Grain Yield,Nitrogen Absorption and Utilization to Elevated CO2 Concentration in Different Rice Varieties

【Objective】Our aim is to investigate the differences in response of grain yield, nitrogen absorption and utilization to FACE (atmospheric CO2 concentration increase) of different rice varieties. 【Method】Six rice varieties, including conventional japonica rice, hybrid indica rice, and conventional indica rice, were used to study the effects of free-air CO2 enrichment (FACE) (atmospheric CO2 concentration increase) on the nitrogen absorption, utilization, and yield of different types of rice.【Result】 1) FACE treatment dramatically increased rice yield by 24.17% on average and the maximum increment was observed in conventional indica rice. Compared with other types of rice varieties, hybrid indica showed the highest grain yield under both FACE and control (CK) treatments. 2) Panicle number was significantly improved in FACE treatment with conventional japonica rice varieties having the maximum increment. Spikelet number per panicle was significantly improved in hybrid and conventional indica rice varieties in FACE treatment. 3) The nitrogen absorption (NA) and nitrogen use efficiency for grain yield (NUEg) were significantly higher in FACE treatment than those in CK treatment. The average increase of NA was 21.23% with the maximum increment in hybrid indica rice varieties. Compared with other rice varieties, conventional indica rice varieties had the highest NA both in FACE and CK treatments. The average increase of NUEg was 7.33% with hybrid indica rice varieties enjoying the maximum increment. The hybrid indica rice varieties had the highest NUEg in FACE treatment and in CK treatment, respectively. 4) Nitrogen content was decreased in FACE treatment with the average decrease of 0.105%, among which the maximum decrease was observed in conventional japonica rice. Dry matter weight was extremely and significantly increased in FACE treatment. The average increase of dry weight was 23.95% with the maximum increment in conventional indica rice varieties. NA of single panicle was significantly improved in FACE treatment with the average increase of 10.79% in conventional indica rice varieties and 13.93% in hybrid indica rice varieties, but NA of single panicle was decrease by 9.60% in conventional japonica rice. FACE treatment significantly increased rice NA intensity with an average increase of 22.29% and the maximum increment was observed in hybrid indica rice varieties. The growth duration was not influenced by FACE treatment in all rice varieties. NAs of stem, leaf and panicle were significantly higher in FACE treatment than in CK treatment with the highest increase of 51.86% in leaf. The largest increase of NA was observed in hybrid indica rice. NA in different rice growth stages was significantly improved with the maximum increment of 108.90% during heading-maturity. The maximum increment of NA from heading to maturity was observed in hybrid indica rice varieties.5) Effects of dry matter weight, NA per panicle, NA intensity, NA of panicle and NA from heading to maturity on NA at maturity were greater than those of nitrogen content, panicle number, growth duration, NAA of leaf, stem and sheath, NAA during transplanting-tillering and tillering-heading. 6) Partial productivity of nitrogen fertilizer (PFPN) was significantly improved in FACE treatment with an average increase of 24.16% and the maximum increment in conventional indica rice. NAA per 100 kg grains was significantly reduced in FACE treatment with an average decrease of 4.7%. The maximum decrease of NAA per 100 kg grains was observed in conventional indica rice varieties.【Conclusion】The results indicated that FACE could markedly increased both grain yield and nitrogen use efficiency in all rice varieties, but the increases varied with the variety types.     

应用Excel进行畜禽性状相关和回归分析

在科研及教学实践中,成熟的统计分析软件正在被广泛使用.Microsoft Excel是功能强大的专业电子表格系统.本文介绍了如何使用Excel 2000的“数据分析”工具进行相关和回归分析.在教学中利用Excel可以较方便地完成复杂的统计计算.     

茶叶中黄酮类化合物对羟自由基清除实现抗氧化功能研究

茶叶中黄酮类化合物是一种天然抗氧化物质，通过对羟自由基清除作用，实现抗氧化作用。茶叶有较强的的抗氧化作用，是因其黄酮类化合物有清除自由基的功能。     

野生茶树菇YW-2液体发酵及子实体氨基酸成分分析

本文在前期试验基础上,进一步研究探讨茶树菇(Agrocybe aegerita)YW-2液体深层培养的优化条件及其子实体氨基酸成分分析.结果表明,经过液体发酵试验,初步获得最佳液体配方为马铃薯麸皮培养液,进而采用正交试验得到优化培养基配方为:A1B2C2D3, 即为马铃薯15g/0.1L,蔗糖2 g/0.1L,KH2PO4 0.2 g/0.1L,豆芽与麸皮汁比3∶ 2;在玉米粒培养基中接入固体原种与液体原种,液体原种转接后更能促进菌丝快速生长;通过子实体营养成分分析,该株野生茶树菇含有9种非必需氨基酸和7种必需氨基酸成分,营养价值丰富.     

大豆CAPS标记快速开发方法的建立与优化

采用生物信息学方法,分析大豆SNP位点序列信息,选择合适的内切酶,进行混样PCR和酶切预筛选,建立了大豆CAPS标记的快速开发方法—gspCAPS(Genome Sequence Pool,CAPS)。设计合成了61对引物,在9个大豆品种中对gspCAPS方法进行了评测。结果表明,该方法显著提高CAPS标记开发的效率,传统CAPS方法效率为40.98%(25/61),该方法效率高达86.21%(25/29),并且标记开发的正确性没有降低,维持在100%。本研究所建立的gspCAPS方法效率高、周期短、成本低,对高效开发CAPS标记具有重大的指导意义和广阔的应用前景。     

Study of Lightning Impulse Breakdown Voltage Correction of PositiveRod-Plate Short Air Gaps on Altitude

The air pressure of the high altitude condition in the artificial climate chamber is simulated, and the laboratory investigation of positive rod plate lightning impulse discharge performance were carried out on the short air gaps of 200 mm, 300 mm and 450 mm according to the test method demanded in IEC 60.1. Based the tested results, it is put forward that the positive lightning impulse breakdown voltage of a short air gap at the air pressure of the high altitude district is a power function of the air pressure with a special index less than 1, which indicates that the effect of the air pressure on the breakdown voltage decreases by 8.7% with the increase of the altitude by 1 kilometer. According to the theoretical analysis, it is proposed that the air pressure suggests the comprehensive changes of the basic atmospheric parameters of the temperature, the reltive air density and the absolute humidity, thus it could be used as the characteristic variable which expresses the effect of the absolute humidity, thus it could be used as the characteristic variable which expresses the effect of the atmospheric condition on the breakdown voltage of an air gap.     

分子标记技术及其在果树种质资源研究上的应用

本文对RFLP、RAPD及AFLP等3种分子标记技术的原理、特点及其在果树品种的鉴定、遗传多样检测、系谱分析、遗传图谱构建等方面应用和进展进行了简要概述，并对应用前景做了展望。     

微卫星DNA标记与鸡肉品质性状的相关分析

本研究测定了隐性白羽和仙居鸡参考家系F2代500羽个体的26个微卫星座位的多态性,分析微卫星标记与鸡肉品质性状之间的相关性,结果发现:位于2号染色体上的标记ADL212分别与pH值和嫩度显著相关;位于3号和5号染色体上的微卫星标记MCW 004和MCW 223与肉色显著相关;与胸肌失水率显著相关的微卫星标记分别为:位于2号染色体的MCW 264和ADL212以及5号染色体的MCW 223和9号染色体的ADL211。