全文获取类型
收费全文 | 19022篇 |
免费 | 1021篇 |
国内免费 | 1507篇 |
专业分类
林业 | 1650篇 |
农学 | 1455篇 |
基础科学 | 787篇 |
1742篇 | |
综合类 | 8785篇 |
农作物 | 1101篇 |
水产渔业 | 709篇 |
畜牧兽医 | 3065篇 |
园艺 | 1450篇 |
植物保护 | 806篇 |
出版年
2024年 | 85篇 |
2023年 | 247篇 |
2022年 | 712篇 |
2021年 | 732篇 |
2020年 | 688篇 |
2019年 | 688篇 |
2018年 | 443篇 |
2017年 | 756篇 |
2016年 | 561篇 |
2015年 | 838篇 |
2014年 | 894篇 |
2013年 | 1260篇 |
2012年 | 1644篇 |
2011年 | 1675篇 |
2010年 | 1678篇 |
2009年 | 1449篇 |
2008年 | 1401篇 |
2007年 | 1274篇 |
2006年 | 1007篇 |
2005年 | 799篇 |
2004年 | 510篇 |
2003年 | 328篇 |
2002年 | 378篇 |
2001年 | 371篇 |
2000年 | 304篇 |
1999年 | 188篇 |
1998年 | 108篇 |
1997年 | 83篇 |
1996年 | 71篇 |
1995年 | 74篇 |
1994年 | 53篇 |
1993年 | 59篇 |
1992年 | 54篇 |
1991年 | 33篇 |
1990年 | 28篇 |
1989年 | 23篇 |
1988年 | 17篇 |
1987年 | 11篇 |
1986年 | 6篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1983年 | 4篇 |
1981年 | 3篇 |
1980年 | 1篇 |
1963年 | 1篇 |
1962年 | 2篇 |
1956年 | 4篇 |
1955年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
111.
Jing YUAN Zhitao WANG Pengxiang DUAN Yousheng XIAO Hongke ZHANG Zhixiong HUANG Ruichun ZHOU Hua WEN Kexiong WANG Ding WANG 《Integrative zoology》2021,16(4):499-511
Geographic variations in the dolphin whistles could be useful in assessing association and isolation among populations. Whistle of free-ranging Indo-Pacific humpback dolphins (Sousa chinensis) among the Pearl River Estuary (PRE), Leizhou Bei (LZB) and Sanniang Bay (SNB) populations were investigated. A total of 2850 whistles with legible fundamental contour were extracted and 15 acoustic parameters were measured. Contrary to SNB, PRE and LZB had the same relative proportion of tonal type compositions with flat and sine representing the most frequent types. The generalized linear model analysis showed significant acoustic difference among populations and tonal types. All frequency parameters in SNB were significantly higher than those in PRE and LZB, where no significant variation was observed in most of the parameters either at the population level or within each tonal type. Canonical discriminant functions analysis showed a smaller difference between PRE and LZB than between PRE and SNB and between LZB and SNB. Compared with previous recordings, recent recordings demonstrated a consistent pattern of becoming higher in whistle frequency parameters in both LZB and SNB populations, suggesting that noise pollution in LZB and SNB increasing with time according to the acoustic niche hypothesis. Dolphin whistle's geographic variations could be shaped by the combined function of the geographical barrier function of the Qiongzhou strait and local ambient noise. Considering the isolated condition and the relatively smaller population size of the humpback dolphin in the SNB, more effective and proactive conservation actions should be taken to prevent the extinction of small populations. 相似文献
112.
依据贵州省松桃县的气候特点和牧草种植需要,用黔育1号菊苣与北林202紫花苜蓿、黔草4号鸭茅分别进行间作试验,从产量、营养品质、生产效益等方面对间作复合群体与3种牧草单播进行比较分析,探讨黔育1号菊苣与不同牧草间作种植模式的增产效益。结果表明,在贵州松桃地区,黔育1号菊苣+北林202紫花苜蓿间作,年均鲜草产量可达87.27 t/hm2,比紫花苜蓿单播产量增加36.17%,生产效益提高20.77%,粗蛋白含量达26.83%。该研究结果可为松桃县种草养畜产业的发展提供技术支撑。 相似文献
113.
滇西北是云南省最为贫困的少数民族聚集地区之一,实行退耕还林政策10多年来取得了巨大的成效,随着退耕还林政策补助陆续到期,部分退耕农户生计将出现困难。对退耕还林后续政策进行分析,认为退耕还林补偿标准与现有物价水平不符,套种的规定过于保守,针对政策上的不足与实施过程中存在的作业设计不够规范、监管不力、档案管理不够规范等问题提出相关对策和建议。 相似文献
114.
通过离子交换色谱法,建立不同厂家阿胶及动物皮胶中Cl~-、SO_4~(2-)、NO_3~-、PO_4~(3-)4种阴离子的测定方法。结果表明,4种阴离子分离效果良好,Cl~-、SO_4~(2-)、NO_3~-、PO_4~(3-)分别在0.000 922~0.023 1、0.001 09~0.027 3、0.000 167~0.004 18、0.000 314~0.007 86 mg/mL浓度范围内线性关系良好,平均加样回收率在98.7%~101.0%。该测定方法结果准确、灵敏、可靠,重现性较好,可用于阿胶及动物皮胶中4种阴离子含量的测定。 相似文献
115.
ZHONG Xing-fu WANG Pei-kun YANG Yong-li QIN Li-li BI Yu-yu ZOU Guang-zhen PENG Hao WEI Ping 《中国畜牧兽医》2015,42(7):1647-1653
In order to investigate the infection status of avian leukosis virus (ALV) and avian reticuloendotheliosis virus (REV) in the major local breeds of Qinzhou,Guangxi,totally 953 samples of egg white,cloaca swab and serum of Ma duck,Shitou goose,Tiejiao-Ma chicken,turkey and pigeon were collected from the representing flocks and detected by the commercial ELISA kits.ALV was isolated for the ALV p27 positive samples by culturing on DF-1 cells,and gp85 gene was sequenced.The results showed that the detections of ALV were negative in the samples except those of Tiejiao-Ma chicken,while REV antibody was found positive in Ma duck,Tiejiao-Ma chicken and turkey.The nucleotide sequences of gp85 gene of two isolates shared 94.5% identity with each other,and shared 86.9% to 94.9% with reference strains.The amino acid sequences of gp85 gene of two isolates shared 91.5% identity with each other,and shared 84.0% to 91.6% with reference strains.There were many variable sites in the hyper variable region hr1 and hr2,and the vr2 and vr3 variable regions were relatively conservative.Phylogenetic tree analysis showed that the two isolates shared the highest homology with SCAU11-XG strain. 相似文献
116.
ZHAO Xin RUAN Zi-yun GUO Zhen-wei ZHOU Wen-ting QIN Xi-ling NIU Xiang-li LU Feng-hua SHI De-shun 《中国畜牧兽医》2016,43(8):1975-1982
In this study,the CDS sequence of buffalo Keap1 gene was cloned and analyzed,then its expression pattern in different tissues was also investigated.A pair of primers of buffalo Keap1 gene was designed based on the nucleotide sequence of Bos taurus Keap1 gene from GenBank,and then the buffalo Keap1 gene was amplified.Using the bioinformation techniques,the gene sequence and the protein structure were analyzed.The expression level of Keap1 gene in different tissues were detected with Real-time quantitative PCR.The results showed that the length of buffalo Keap1 gene coding sequence was 1 875 bp and encoded 624 amino acids.The multiple sequence alignment results showed that buffalo Keap1 gene shared 99%,96%,92% and 90% of similar nucleotide sequence with that of Bos taurus,Ovis aries,Sus scrofa and Homo sapiens,respectively.And the phyogenetic tree also showed the conservatism between several different species.The second structure of buffalo Keap1 protein was predicted as 24 alpha regions,40 beta regions,38 turn regions and 27 coil regions.In addition,the results of Real-time quantitative PCR showed that Keap1 mRNA exists in all seven tissues,but the most abundant expression was in heart and the minimal expression was in liver and spleen.The results provided an foundation for further study of Keap1-Nrf2-ARE signal pathway,for enhancing the ability of antioxidant of buffalo embryo in vitro culture. 相似文献
117.
118.
119.
由于激光具有功率高、相干性好、光谱线宽窄、光谱范围广等特点,其在土地保护、农作物生长及害虫防治等领域应用广泛.文章综合国内外激光技术在农业领域的应用情况,介绍了已经获得应用的激光诱导击穿光谱仪、激光测距仪、激光雷达系统、激光补光灯等设备在土壤检测、土地平整、激光诱变育种、植物补光和虫害防治等领域发挥的作用,对激光技术对农业领域的推动作用,特别是激光技术在吉林省农业应用方面的情况进行了展望. 相似文献
120.
本研究以三七(Panax notoginseng)植株及其产品和近缘种为研究对象,采用L9(3 4)正交试验对三七素的提取工艺进行了优化,然后利用HPLC法检测三七素含量,并对各样品中的三七素含量进行了比较分析。结果表明:优化后的三七素提取条件为超纯水、料液比1∶20(g/mL),提取1次(10 min);三七素在三七花蕾中的含量最高,其次是根状茎和主根,但是三七茎叶中也有较高含量的三七素,说明三七花蕾和茎叶均可作为三七素的提取原料;在三七产品中三七花的三七素含量最高,达到3.33%,其次是剪口(根状茎)、筋条(侧根)和须根;但三七素的含量与三七个头大小(头数)并无明显的相关性;在三七近缘种姜状三七(P. zingiberensis)和野三七(P. vienamensis var. fuscidiscus)主根、珠子参(P. japonicus var. major)根状茎中的三七素含量显著低于三七主根三七素含量,也显著低于三七其他部位的三七素含量,说明三七是最佳的三七素来源植物。三七素的含量主要受到物种和部位的影响,与产品规格无关。本文所建立的三七素提取方法和HPLC检测方法,能够快速简便、稳定可靠测定样品中三七素含量;并可为三七素相关标准的建立奠定基础,为三七的综合利用开发提供依据。 相似文献