甘肃省秦安县苹果叶缘焦枯死亡原因调查

通过对甘肃省秦安县苹果叶缘焦枯死亡的原因初步调查.结果表明:苹果叶缘焦枯是由于土壤中单盐(NaCl)毒害所致,而土壤中盐分的来源是通过微咸水和中度咸水灌溉累积造成的,随年限的增加,危害日趋严重.从土壤的分析结果看,项目区果园土壤盐分组成类型为:0～20cm和40～60cm土层为氯化物-苏打-硫酸盐型;20～40cm土层为苏打-氯化物-硫酸盐型.pH＜8.5,土壤表层盐分的含量均小于0.20%,ESP值小于10,HCO3-/(Cl- SO42-)＜0.5,与生长良好的果园区土壤相比有轻微的盐化和碱化现象.     

不同氮、钾供肥量对厚皮甜瓜产量和品质的影响

研究了富磷土壤条件下不同氮、钾供肥量对厚皮甜瓜产量和品质的影响.结果表明:在富磷条件下,随供氮量的增加,厚皮甜瓜第一雌花发育时间和果实成熟期相应延迟;供氮量低于393.8 kg/hm2时,增加供氮量可提高产量、增加单果重、Vc含量和蛋白质含量;供氮量超过330.1 kg/hm2时,总糖含量会随着供氮量的增加而降低,硝酸盐含量随之显著增加.供钾量的增加会使厚皮甜瓜第一雌花发育时间和果实成熟期缩短,供钾量在634.9 kg/hm2以下时,随着供钾量的增加单果重、产量增加,硝酸盐含量则随之降低.供氮量在393.8 kg/hm2以下时,蛋白质含量随着供钾量的增加而增加.从产量和品质方面综合分析,供氮量为393.8 kg/hm2,供钾量为611.9 kg/hm2时综合效果最好,产量可达45 663.5 kg/hm2,总糖含量达17.88%,Vc含量达到最高水平35.94 mg/100g,蛋白质含量达606.36 mg/100g,硝酸盐含量低于600 mg/100g.     

甜瓜辐射花粉诱导单倍体的影响因素研究

以Co60γ射线为辐射源,研究了辐射剂量200,300,500,800,1 200,1 500和2 000 Gy对甜瓜单倍体诱导率的影响. 同时在辐射花粉授粉6 h后,用1,10,50,100和200 mg/L的2,4-D,以浸蘸柱头和节间注射2种处理方法,研究2,4-D对甜瓜辐射花粉诱导单倍体的影响.研究结果表明,辐射剂量在200～1 500 Gy时,坐果率低于对照(96.7%),但不同剂量间坐果率没有明显的差异,都达到80%以上;而在2 000 Gy时坐果率明显降低,仅为63.3%.2,4-D的两种处理方法在低浓度(1 mg/L)时,对坐果率没有影响;但随2,4-D处理浓度的升高,坐果率呈下降趋势.浸蘸柱头对出胚率和植株再生率没有明显影响,而在100 mg/L浓度下采用节间注射处理时出胚率和植株再生率分别为2.8%和1.09%,明显高于其他处理.     

不同覆土材料对双孢蘑菇栽培影响研究

在湖北荆门对当地的3种土样及其和砻糠混合组成的6种覆土材料进行对比试验,通过试验棉花地土最适合作为双孢蘑菇简易覆土材料。     

陇东马牙枣幼胚培养

以陇东马牙枣盛花后50～60d的幼胚为试材,采用L9(3)4正交设计,筛选适宜的幼胚萌发培养基。结果表明,基本培养基、激素配比、蔗糖的含量对幼胚萌发都有显著的影响;其中MS-H培养基的萌发率最高为47.62%;ZT最适宜含量为0.5mg/L,IAA最适宜含量为0.6mg/L;较高的蔗糖含量(50g/L)有利于胚的萌发。幼胚萌发的适宜培养基为MS-H+ZT0.5mg/L+IAA0.6mg/L+LH0.5g/L+蔗糖50g/L+琼脂4.0g/L,剥去种皮,幼胚萌发效果较好。当胚萌发后,将胚从胚乳中或愈伤组织中剥离继续诱导愈伤组织,再经诱导不定芽,生根获得正常植株。     

无籽西瓜新品种新优39号的选育

新优39号是新疆石河子市蔬菜研究所2003年育成的中熟无籽西瓜一代杂种。通过品种比较试验、区域试验和生产试验,该品种表现优良,全生育期90d左右,果实发育期约33d。果实圆形,果皮绿底覆深绿色条带,光滑美观,厚约1.0cm;瓤色鲜红,质脆多汁,口感风味好,中心可溶性固形物含量11.4%左右;白色秕籽小而少。平均单果重4kg,667m2产量3500kg左右。综合性状超过对照。2007年2月通过新疆维吾尔自治区农作物品种审定委员会审定。     

Role of estrogen in alleviating hemorrhagic shock-induced organ injury

Estrogen has many biological activities and extensive clinical applications. Hemorrhagic shock-induced major organ dysfunction and injury, which are related to sex differences, play a triggering role in irreversible shock. The present article reviews the role of estrogen in alleviating hemorrhagic shock-induced organ injury by analyzing and summarizing the recent studies, thus expanding the clinical application of estrogen and providing a novel approach for treatment of severe hemorrhagic shock.     

Effects of USP9X down-regulation on apoptosis and invasion ability of gastric carcinoma AGS cells

AIM: To investigate the effects of ubiquitin-specific peptidase 9, X-linked (USP9X) down-regulation on apoptosis and invasion ability in gastric carcinoma cells, and to explore its possible molecular mechanisms. METHODS: USP9X small interfering RNA (siRNA) and control siRNA were used to be transfected into gastric carcinoma AGS cells. The cells were divided into 3 groups, including untreated AGS group, control siRNA group and USP9X siRNA group. The expression of USP9X at mRNA and protein levels in the AGS cells with different treatments was determined by real-time PCR and Western blot. The cell viability was analyzed by CCK-8 assay. Flow cytometry and Boyden chamber were employed to examine the apoptosis and invasion ability of the AGS cells. RESULTS: USP9X siRNA significantly down-regulated the expression of USP9X at mRNA and protein levels in the AGS cells. Down-regulation of USP9X markedly induced apoptosis and reduced invasion ability of the gastric carcinoma AGS cells. Notably, down-regulation of USP9X significantly reduced the protein expression of Mcl-1 and MMP-2, but markedly increased the protein level of Bax. CONCLUSION: USP9X may be a key regulator for apoptosis and invasion in gastric carcinoma.     

Mechanism of AKT1 negatively regulating breast cancer cell migration

AIM: To investigate the molecular mechanism and downstream signaling pathway by which AKT1 inhibition regulates breast cancer cell migration. METHODS: RNA interference was used to knockdown the expression of AKT1. Western blot assay was performed to examine the expression of AKT1 total protein, β-catenin total protein and β-catenin nuclear protein. Immunofluorescence was used to examine the cellular localization of β-catenin. Transwell assay was used to investigate whether β-catenin nuclear accumulation as an alternative pathway was responsible for breast cancer metastasis induced by AKT1 inhibition. RESULTS: The total protein expression of AKT1 was decreased in MCF-7 and MDA-MB-231 cells treated with AKT1 siRNA. A significant increase in the protein expression of β-catenin was observed in MCF-7 cells and MDA-MB-231 cells treated with AKT1 siRNA. Immunofluorescence staining showed that MCF-7 cells and MDA-MB-231 cells displayed strong β-catenin staining in the cytoplasm and nucleus after knockdown of AKT1 expression. The ability of tumor cell migration increased dramatically after treated with AKT1 specific siRNA in the breast cancer MCF-7 cells and MDA-MB-231 cells in Transwell assay. XAV-939 reversed breast cancer cell migration induced by knockdown of AKT1 expression. CONCLUSION: β-catenin nuclear accumulation contributes to AKT1 inhibition-mediated breast cancer cell migration.     

日本苦瓜种质资源种子脂溶性成分分析鉴定

以日本苦瓜种质(Y105、Y112、Y115和Y116)为试材,采用气相色谱-质谱技术分析种子脂溶性化学成分及相对含量差异。结果表明:不同日本苦瓜种质脂溶性化学成分种类和相对含量存在一定程度的差异。从Y105、Y112、Y115和Y116中分别鉴定出23、16、19、20种化合物,共有33种成分。5种脂溶性化学成分为Y105中特有;5种脂溶性化学成分为Y112中特有;1种脂溶性化学成分为Y116中特有。其中主要化学成分为25,26-二氢ela甾醇、Ela甾醇、β-生育酚、γ-生育酚、24-亚甲基环木菠萝烷醇。该研究可为苦瓜产品开发提供依据。