半滑舌鳎生物学及养殖生态学研究进展

半滑舌鳎(Cynoglossus sem ilaevisGünther)属鲽形目,舌鳎科,舌鳎属,三线舌鳎亚属,俗称牛舌头、鳎米等。分布于朝鲜、中国、日本的近海,为近海常见的暖温性大型底层鱼类。我国主要分布于渤海、黄海海域。半滑舌鳎栖息于泥沙质海底,食性广,抗逆性强,摄食底栖无脊椎动物。半滑舌鳎是我国已报告的25种舌鳎属种类中个体最大的一种,体长一般为20~50 cm,最大体长可达80 cm。半滑舌鳎生长速度快,肉质细腻、鲜美,营养丰富,经济价值很高,适合于我国沿海养殖[1]。我国有关半滑舌鳎的研究始于20世纪80年代末90年代初,中国水产科学研究院黄海水产研究…     

北太平洋鱿鱼肌肉蛋白质凝胶特性的研究

以北太平洋鱿鱼为原料,从擂溃工艺、弹性补强剂、品质改良剂以及加热方式对鱿鱼肌肉蛋白质凝胶特性进行了较为系统的研究.先利用正交试验设计分别确定擂溃参数,淀粉、植物蛋白和水的添加量,并在此基础上通过单因素试验确定转谷氨酰胺酶、柠檬酸钠、多磷酸盐的添加量及加热方式.研究结果表明,在试验范围内,擂溃温度3 ℃,空溃5 min,盐溃10 min;添加8%植物蛋白,10%淀粉,20%水分,凝胶弹性较好.采用二段加热,柠檬酸钠、转谷氨酰胺酶、多磷酸盐的添加量分别为0.2%、0.2%、0.3%,鱼糜的凝胶性能较好.     

梨叶片中苹果褪绿叶斑病毒的引物原位标记检测

 选用带苹果褪绿叶斑病毒的香梨叶片,制备成适合进行原位RT-PCR的石蜡切片。设计特异引物,利用引物原位标记技术对切片组织中的病毒进行了检测研究,结果表明,PRINS-FITC染色法和PRINS-Rhodamine染色法均能获得良好的检测效果,有病毒部位分别显示绿色荧光和红色荧光,而且荧光出现的位置与原位RT-PCR检测的结果一致。由此证明引物原位标记技术可用于果树病毒的原位检测。     

Mechanism of mesenteric lymph reperfusion aggravates multiple organ injury in superior mesenteric artery occlusion shock rats

AIM: To explore the mechanism of mesenteric lymph reperfusion (MLR) aggravates multiple organ injury in superior mesenteric artery occlusion (SMAO) shock rats. METHODS: Male Wistar rats were randomly divided into 4 groups: in sham group, only anesthetization and operation were performed; in MLR group, occlusion of mesenteric lymphatics (ML) for 1 h followed by 2 h of reperfusion; in SMAO group, occlusion of superior mesenteric artery (SMA) for 1 h followed by 2 h reperfusion; in MLR+SMAO group, occlusion of SMA and ML for 1 h followed by 2 h of reperfusion. The homogenates of liver, kidney, myocardium and lung were prepared for determining the activities of free radical, nitric oxide, myeloperoxidase (MPO) and cell membrane ATPase. RESULTS: The MDA, NO contents and NOS, MPO activities of multiple organic homogenate in SMAO and MLR+SMAO group were higher than those in sham and MLR group, and these indexes in MLR+SMAO were increased significantly than those in SMAO group. The SOD and ATPase activities of muliple organic homogenate in SMAO and MLR+SMAO group were lower than those in sham and MLR group, and those in MLR+SMAO group was decreased obviously than those in SMAO group. CONCLUSION: The MLR enhances the multiple organ free radical injury, NO synthesis and release, PMN detention and decreases the activity of cell membrane ATPase, aggravating the major organs injury in SMAO shock rats. Intestinal lymphatic pathway plays an important role in the pathogenesis of SMAO shock.     

黄瓜耐弱光性的多元统计分析

 利用遮阳网模拟弱光条件, 调查18份黄瓜自交系苗期生长发育和生理生化指标, 采用多元统计分析方法评价黄瓜品系耐弱光性强弱。结果表明, 参试品系的13个指标在品系间存在显著差异, 利用因子分析将这些指标综合成5个主因子, 累计贡献率达85.62%。对旋转后的因子得分进行聚类分析, 供试品系可分为4类: 耐弱光材料M₂₂、M₄₃、M_a9; 较耐弱光材料M₈、M₁₁、M₃、M₄、M₃₄; 弱光中度敏感材料M₁₂、M₂₁、M₁₄、M₂、M_a1、M₂₈和弱光敏感材料M₁₀、M₄₄、M₃₂、M₃₆。经过弱光处理后耐弱光指数较大、光合速率降低幅度较小的品系耐弱光性强, 应结合耐弱光指数与光合、生长等因子综合进行黄瓜耐弱光性鉴定。     

甘蓝自交不亲和决定因子的体外表达和相互作用的检测

 S位点受体激酶(SRK)和S位点富含半胱氨酸蛋白/S位点蛋白11(SCR/SP11)分别为甘蓝自交不亲和(self-incompatibility, SI)信号传导的雌雄决定因子。为了深入研究两者的作用机理和进行人工调控,本研究以结球甘蓝ZQ为材料,利用pET·NusA融合蛋白表达系统,将包含SRK胞外域和跨膜域的mSRK蛋白和SCR蛋白在大肠杆菌BL21中融合表达,经SDS-PAGE电泳检测表达出融合蛋白大小分别约为116 kD和74 kD。进一步将两融合蛋白进行体外相互作用的检测,结果表明mSRK与SCR蛋白能够相互结合形成稳定的复合体,这为下一步实现SRK-SCR复合体聚合与解离的人为调控提供了技术平台。     

Asymmetric dimethylarginine protects PC12 cells against glutamate-induced excitotoxic damage

AIM: To investigate the effects of asymmetric dimethylaoyoinine (ADMA) on glutamate-induced PC12 cell damage and its mechanisms. METHODS: PC12 cells were treated with different concentrations of glutamate as an in vitro excitotoxic trauma model. The cell viability was measured by MTT assay. Glutamate cytotoxicity was evaluated by lactate dehydrogenase (LDH) release assay. Intercellular reactive oxygen species (ROS) was detected by dihydrorhodamine123 (DHR) staining and flow cytometric (FCM) analysis. Nitric oxide synthase (NOS) activity and nitric oxide (NO) production were detected by using commercial kits with a spectrophotometer. RESULTS: Glutamate at concentrations of 1 mmol/L to 6 mmol/L dose-dependently decreased PC12 cell viability. Pretreatment 30 min with ADMA prior to administration of glutamate significantly attenuated the inhibition of cell viability, LDH release and ROS accumulation induced by glutamate. Pretreatment with ADMA significantly inhibited the increases in NOS activity and NO production caused by glutamate. CONCLUSION: ADMA obviously protects PC12 cells against glutamate-induced excitotoxicity by inhibiting NOS activity, overproduction of NO and accumulation of intracellular ROS.     

Jagged1 knocking down in endothelial cells accelerates PDGF induced proliferation and migration of vascular smooth muscle cells in rats

AIM: To investigate the effect of Jagged1 expression in endothelial cells (EC) on platelet derived growth factor (PDGF) induced proliferation and migration of vascular smooth muscle cells (VSMC) in rat.METHODS: Rat aorta EC was inoculated in the lower chamber and VSMC were in the upper chamber of the cell coculture system. Three groups were divided: control, sicontrol and siJagged1. The EC Jagged1 protein expression was assayed by Western blotting to evaluate small RNA interfering (RNAi) efficiency. After the cells were cocultured with PDGF for 24 h, the proliferation and migration of VSMC were respectively evaluated by ［3H］-TdR incorporation and migrating cells counting. Protein expression of α-SM-actin in VSMC was assayed by Western blotting. RESULTS: The Jagged1 protein expression in EC was significantly lower in siJagged1 group than that in control group (0.26±0.02 vs 0.67±0.02, P<0.05), and no statistic significance was observed between control and sicontrol groups. The VSMC ［3H］-TdR incorporation and migration were higher in PDGF +siJagged1 group than those in PDGF group {［3H］-TdR incorporation (23 074±2 702) counts·min-1·well-1 vs (16 442±1 803)counts·min-1·well-1, n=5, P<0.05; migration (27±4) cells/field vs (15±3)cells/field, n=5, P<0.05}. The α-SM-actin protein in VSMC was lower in PDGF + siJagged1 group than that in PDGF group (0.25±0.06 vs 0.49±0.04, n=3, P<0.05).CONCLUSION: Jagged1 knock down in rat EC accelerates PDGF induced proliferation and migration of VSMC. These results suggest that Jagged1 expression in EC plays an important role in maintaining VSMC contract phenotype and inhibiting VSMC overgrowth after arterial injury.     

早熟大白菜新品种中白62号的选育

中白62号是以矬菜类型早熟材料自交系B60533和青麻叶类型材料自交系B60532为亲本配制而成的早熟大白菜一代杂种。成熟期60 d(天)左右。株型紧凑,株高39 cm,开展度57 cm,外叶深绿色,少皱、有光泽,叶缘波褶小而浅,叶柄浅绿色。叶球直筒形,顶部舒心,球高35 cm,横径13 cm,球形指数2.8,叶球净质量1.6 kg左右。每667 m2净菜产量4 600 kg左右,高抗病毒病、霜霉病、黑腐病,球叶不易发生干烧心。质地脆嫩,品质优良,适宜在辽宁、河北、天津、北京、陕西、贵州等省市及山东、黑龙江、河南、山西等省的部分地区秋季种植。     

几个鸢尾新品种栽培研究

对几种在郑州地区新引进的德国彩花鸢尾进行了栽培研究。结果表明:不朽白和黑骑士生长较旺,花朵较大,但开花率低,花期较短,繁殖系数低;黄娃娃和血石长势中庸,花朵中等,开花率高,花期较长,繁殖系数也高;金娃娃与黄链长势中庸偏下,花朵较小,但花期较长,金娃娃繁殖系数中等,黄链繁殖系数最低;紫边白植株矮小,长势较弱,花朵也小,但花期较长,繁殖系数较低。无论任何品种在郑州地区均能良好生长,且其生长、开花、繁殖均与土壤肥沃、疏松、排水良好呈正相关。