全文获取类型
收费全文 | 54383篇 |
免费 | 3617篇 |
国内免费 | 4642篇 |
专业分类
林业 | 4256篇 |
农学 | 3105篇 |
基础科学 | 2557篇 |
5570篇 | |
综合类 | 26830篇 |
农作物 | 4231篇 |
水产渔业 | 2514篇 |
畜牧兽医 | 7526篇 |
园艺 | 3900篇 |
植物保护 | 2153篇 |
出版年
2024年 | 428篇 |
2023年 | 1085篇 |
2022年 | 2515篇 |
2021年 | 2568篇 |
2020年 | 2395篇 |
2019年 | 2243篇 |
2018年 | 1550篇 |
2017年 | 2705篇 |
2016年 | 1700篇 |
2015年 | 2723篇 |
2014年 | 2843篇 |
2013年 | 3506篇 |
2012年 | 4614篇 |
2011年 | 4817篇 |
2010年 | 4287篇 |
2009年 | 3902篇 |
2008年 | 3770篇 |
2007年 | 3478篇 |
2006年 | 2964篇 |
2005年 | 2259篇 |
2004年 | 1410篇 |
2003年 | 955篇 |
2002年 | 1054篇 |
2001年 | 1042篇 |
2000年 | 839篇 |
1999年 | 333篇 |
1998年 | 112篇 |
1997年 | 86篇 |
1996年 | 59篇 |
1995年 | 66篇 |
1994年 | 50篇 |
1993年 | 35篇 |
1992年 | 41篇 |
1991年 | 40篇 |
1990年 | 21篇 |
1989年 | 26篇 |
1988年 | 19篇 |
1987年 | 21篇 |
1986年 | 23篇 |
1984年 | 4篇 |
1981年 | 11篇 |
1979年 | 1篇 |
1973年 | 1篇 |
1965年 | 2篇 |
1963年 | 3篇 |
1962年 | 11篇 |
1957年 | 1篇 |
1956年 | 16篇 |
1955年 | 5篇 |
1954年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 343 毫秒
101.
AIM:To study the effect of TGF-β1 and TNF-α antisense PS-ODNS on ex vivo expansion of hematopoietic stem/progenitor cells (HSPC). METHODS:CD34+cells were purified from fresh umbilical cord blood by immunomagnetic beads, and mononuclear cells were purified from bone marrow by Ficoll-hypaque. The effects of TGF-β1 and /or TNF-α antisense PS-ODNS on ex vivo expansion of CD34+ cells、CFU-GEMM、CFU-GM、CFU-E and BFU-E were detected by using liquid and semi-solid culture systems.RESULTS:TGF-β1 antisense PS-ODNS cooperated with cytokines increased the number of CD34+ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E, which was as 4, 2.6, 2.7, 1.8, 2.1 times as that of the control (the cytokines combination), respectively. TNF-α antisense PS-ODNS cooperated with cytokines respectively increased the number of CD34+ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E by 4, 2.9, 2.6, 1.7, 1.8 times as that of the control. The above two antisense PS-ODNS cooperated with cytokines could respectively increased the number of CD34+ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E by 5.3, 2.1, 2.7, 1.9, 1.8 times as that of the control.CONCLUSION:Inhibition of endogenous TGF-β1 and TNF-α by antisense PS-ODNS will be one of the effective methods to expand HSPC ex vivo. 相似文献
102.
AIM:To investigate the effects of FK506 on anti-glomerular basement membrane(GBM) nephritis in rats. METHODS: Anti-GBM nephritis model was elaborated by rabbit anti-rat GBM antibody injection in SD rats in this study. The rats were divided into three groups: FK506 treated group(0.5 mg·kg-1·d-1, sc), untreated nephritis control group and normal control group. FK506 was administered daily six hours after injection of anti-GBM IgG. All the rats were observed urinary protein at the 4th day, the 14th day and the 21st day. At the same time, the kidney specimens were collected, and T cell transforming function was also monitored.RESULTS:Rats injected with rabbit anti-GBM Ab developed heavy proteinuria by 4 days, and serum creatinine and serum urea appeared which kept on the rising. Glmerular hypercellularity, crescents, and protein casts were observed in nephritic rats. By electron microscopy, the thickening of GBM and loss of foot processes were seen. T cell transforming function was higher than normal. But, all pathological changes obviously turned for the better in FK506 treated group. CONCLUSION:FK506 could inhibit the progression of rat anti-GBM nephritis. 相似文献
103.
AIM:To investigate therapeutic effects of recombinant human growth hormone(rhGH) on rat sepsis and its possible mechanisms.METHODS:Mean arterial pressure (MAP), levels of plasma TNFα, IL-1β and endotoxin, leukocyte count and survival rate within 1 week were determined after E. coli injection among control group, sepsis group and sepsis+rhGH group.RESULTS:(1)rhGH diminished the decrease of MAP, reduced plasma endotoxin and TNFα levels and increased neutrophil ratio in total leukocytes in sepsis rat. rhGH increased survival rate within 1 week on sepsis rat. (2)No changes were found in IL-1β level among the three groups.CONCLUSION:rhGH showed desirable beneficial effects on rat sepsis, which may attribute to: improving circulatory function;maintaining intestinal mucosa barrier, attenuating bacteria/endotoxin translocation and inhibiting the production and release of TNFα. 相似文献
104.
105.
106.
陕甘宁老区脆弱生态环境定量评价——以榆林、延安两市为例 总被引:4,自引:0,他引:4
位于黄土高原中部的陕甘宁老区生态环境极为脆弱 ,近年来由于气候、人类开发资源等自然和人为原因 ,使生态环境的脆弱程度升高。选定年降水量、年均温、蒸发量等 8个指标 ,定量评价各县 1 970 - 2 0 0 0年的脆弱度状况 ,结果表明榆林、延安两市生态环境整体脆弱 ,脆弱度存在空间差异但差异不明显 ,时间段上的波动幅度不大。陕甘宁老区脆弱生态环境具有不稳定性 ,对外界干扰较敏感。 相似文献
107.
不同粘度矿物油助剂对除草剂活性的影响 总被引:5,自引:1,他引:5
室内及温室内测定了10种不同粘度矿物油助剂对除草剂防除苘麻和稗草药效的影响及对玉米苗期的安全性。结果表明,10种矿物油助剂均不同程度地增加了氰草津和烟嘧磺隆防除两种杂草的活性。其中粘度为46 mm2/s (40℃)的矿物油乳剂(MOC46)对供试除草剂药效的增强作用最为明显,其次为粘度32 mm2/s (40℃)的矿物油乳剂。10种助剂对氰草津药效的增强作用大于对烟嘧磺隆。MOC46对除草剂活性的增加由高到低依次为:莠去津、氰草津>莠去津+氰草津、莠去津+烟嘧磺隆、氰草津+烟嘧磺隆>烟嘧磺隆;对玉米苗期安全性依次为:莠去津、烟嘧磺隆、莠去津+烟嘧磺隆>莠去津+氰草津、氰草津+烟嘧磺隆>氰草津。氰草津与MOC46混用对玉米苗期药害较重。 相似文献
108.
109.
苏云金芽孢杆菌cry基因在大肠杆菌中表达产物和生物活性 总被引:4,自引:0,他引:4
研究了几种Bt cry基因于大肠杆菌(Escherichia coli)中表达产物在pH 10.0的50mmol/L碳酸钠和20mmol/L乙醇胺溶解液中的溶解性 ,发现同样的Cry蛋白在碳酸钠中的溶解度大于乙醇胺。通过胰蛋白酶消化 ,明确Cry1Ca7、Cry1Ia8酶解产物为 38kD多肽 ;Cry1Ie1、Cry1Cb2、Cry2Ab4酶解产物为 41kD多肽 ;Cry1Ac酶解产物为60kD多肽。采用FPLC层析方法对 6种原毒素及其酶解后得到的毒素多肽进行了分离纯化 ,比较了原毒素和毒素的杀虫活性的差异。其结果表明 ,Cry1Ac的原毒素和毒素对棉铃虫初孵幼虫的校正死亡率均为 100% ,Cry2Ab4的原毒素的毒力高于其酶解毒素。 相似文献
110.