杉木木质化过程特异表达基因文库的构建与分析

研究木质部木质化过程及其生物学特性对于了解木本植物水分和矿物质的吸收及运输机制、木材形成的遗传控制、改良材性及提高木材生长量具有十分重要的意义。为了获得杉木木质化过程中特异表达的基因,本研究利用抑制差减杂交技术,以杉木突变体独干杉无性系为测试方(Tester),正常的句容0号无性系为驱动方(Driver),构建了正向和反向两个差减文库,分别获得了618和409个克隆。利用通用引物T7和SP6进行PCR及EcoRⅠ酶切鉴定文库重组子,结果证明所构建的文库符合要求,具有代表性。该文库的成功构建为获得杉木木质化过程中特异表达的基因及深入了解木本植物木质化的机理奠定了基础。     

高校毕业生就业信息的搜集、整理、利用及有效性控制

高校毕业生就业信息的搜集、整理、利用及有效性控制是高校就业指导工作的重要组成部分。本文对就业信息搜集、整理、利用及有效性控制等一系列问题进行研究,提出了相应的对策和措施,并介绍了工作实践经验。     

Effects of Interaction of Soil Moisture and Organic Matter on Powdery Mildew Disease and Growth of Heracleum moellendorffii Hance

Taking annual Heracl'eum moellendorffii Hance as the material, this research studied the epidemic dynamics of H. moellendorffii Hance powdery mildew and plant growth by using the interactive methods of soil moisture and soil organic matter. The results showed that the interaction of higher soil moisture content (60％-80％) and soil organic matter content (75-100 g · kg-1) had few diseases, dosease index and area under disease progression curve (AUDPC) compared with those of other treatments, thus could effectivley control powdery mildew disease. Moreover, higher soil moisture and organic matter content increased the yield, contents of soluble sugar, soluble protein, vitamin C and coumarin in H. moellendorffii Hance.     

硬质合金刀片切削力控制器的设计及仿真

针对硬态切削条件下的切削力控制问题,建立了控制模型,使用MATLAB/SIMULINK设计了PID/PD控制器,对径向力的控制效果进行了仿真。结果表明:通过调节进给量能够有效地控制径向切削力变化,同时,能够较好地控制刀具的磨损速率。     

筇竹QtKAT1基因的克隆与表达分析

为探究竹类植物K+调控机理及K+通道的系统,本研究以筇竹(Qiongzhuea tumidinoda)为试验材料,对筇竹QtKAT1基因分离克隆及表达分析.通过克隆获得筇竹QtKAT1基因,全长为3 124bp,含2 235 bp的最大开放阅读框,编码744个氨基酸.生物信息学分析表明QtKAT1基因预测编码蛋白分子质...     

环磺酮对八种杂草生物活性的室内测定及田间应用效果

正环磺酮属于对羟基苯基丙酮酸双氧化酶抑制剂,是由拜耳公司开发的一种苯甲酰环己二酮类除草剂,其中国专利将于2019年9月8日到期,目前国内对环磺酮的研究主要集中在合成、色谱分析以及残留等方面(余玉等,2017),但其生物活性以及在玉米田的应用效果未见报道。本研究在温室内测定环磺酮杂草防除谱及其对玉米的安全性,同时在田间对其应用效果进行测定,以期为该药剂在中国的推广以及大面积应用提供理论依据。     

作物地膜覆盖技术适宜性及其在东北春玉米上的应用

为从源头解决地膜覆盖技术泛用、滥用问题,提高作物地膜覆盖技术的合理利用性。研究提出作物地膜覆盖适宜性的概念,将其定义为"地膜覆盖技术对作物自身环境要素需求与所在地提供环境要素差异的补偿程度"。该研究以东北春玉米为例,构建春玉米地膜覆盖适宜性的评价体系,通过数据检索的方式收集东北地区田间试验中春玉米地膜覆盖功能数据,明确作物地膜覆盖与不覆盖农田土壤温度和水分、经济产量相关关系,量化作物地膜覆盖的增温保墒、增产功效,筛选春玉米地膜覆盖技术适宜性评价指标,并计算地膜覆盖技术适宜指数,参照作物需求阈值标准和经济效益,明确东北不同熟期春玉米地膜覆盖种植范围,形成春玉米地膜覆盖综合适宜性区划。东北地区春玉米不同生育阶段地膜覆盖耕作层土壤温度（土壤含水率）与裸地土壤温度（土壤含水率）及产量与≥10 ℃积温存在良好的线性关系（P < 0.01）。基于春玉米地膜覆盖适宜性的评价体系,计算了地膜覆盖适宜指数,完成东北地区春玉米地膜覆盖综合适宜分区。作物地膜覆盖适宜性相关研究可为中国地膜投入量零增长和地膜污染综合防控提供可靠技术支撑。     

福尔马林对鳜鱼苗种车轮虫、斜管虫病的防治研究

福尔马林对车轮虫、斜管虫有极强的杀灭能力,对鳜鱼苗种的安全浓度为2.04ppm.在网箱、孵化缸中的泼洒浓度为150～200ppm,池塘中为20ppm.试验结果表明:福尔马林对鳜鱼苗种车轮虫、斜管虫病的治疗效果好于硫酸铜、硫酸亚铁合剂及硫酸铜。     

Characterization of Collagen Enzymatic Hydrolysates Derived from Lizardfish (Synodus fuscus) Scales

Acid soluble collagen was extracted from the scales of lizardfish and characterized with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid analysis. After 8 h of collagen hydrolysis, hydrolysates had an estimated degree of hydrolysis (DH) from 4 ± 0.05 to 25 ± 0.63% (p < 0.05). All hydrolysates had angiotensin converting enzyme inhibitory and antioxidant activity. These activity levels showed little change after treatment with gastrointestinal proteases. Results indicated that the lizardfish by-products may be improved by enzymatic treatment with acid-soluble collagen from lizardfish scales.     

Can fermented soybean meal and squid by‐product blend be used as fishmeal replacements for Japanese flounder (Paralichthys olivaceus)?

An experiment was carried out to evaluate fermented soybean meal and squid by‐product blend (1:1) (FP) as replacement of fishmeal (FM) for Japanese flounder, Paralichthys olivaceus. Five isocaloric (19 kJ g^?1) diets were prepared by replacing 0 (FP0), 12 (FP12), 24 (FP24), 36 (FP36) and 48% (FP48) FM protein with FP. Triplicate groups of juveniles (mean weight of 3.9 g) were delivered the test diets for 8 weeks in a flow‐through sea water system. The results showed that there were no significant differences (P > 0.05) among the growth rates of fish fed FP0, FP12, FP24 and FP36 diets. Growth and nutrient utilization parameters were significantly reduced in fish fed FP48 diet. Although, whole body proximate composition of fish were not significantly affected by the dietary treatments compared to the control; methionine and phenylalanine contents were significantly decreased in FP48 group. Protein retention was also significantly decreased in the similar group of fish. Dietary treatments did not alter most of the plasma metabolites, while some of the health parameters were improved in the replacement groups. Results suggested that FP is a potential candidate for alternative protein ingredient in aquafeed and can replace 36% FM protein in the diet of Japanese flounder.