玉米雄性不育突变体mi-ms-3的遗传分析及分子鉴定

Maize is one of the best crops in the utilization of heterosis. Male sterile lines are important germplasms for the hybrids production. A male sterile mutant named mi-ms-3 was obtained by screening in a mutator insertion library. The number of male anthers in tassel decreased and not exserted. There were few anthers with only two pollen sacs in the mutant tassels, and some of the anthers were degenerated to membranous and formed filaments at their ends. Although pollens in the anthers could be stained by I₂-KI, pollen shedding was abnormal and the number of pollen grains decreased. The number of silks in the ear of the mutant increased, and there was a sterile grain on both sides of the maturated kernel. Fertility of F₁ plants, which were obtained by hybridization between mi-ms-3 and maize inbred Mo17, was normal. Genetic analysis of F₂ population showed that the mutant phenotype was controlled by a recessive gene. The candidate gene was preliminarily mapped on the long arm of chromosome 3 by BSA and it was located between a SSR marker and an Indel marker with a distance of 1.5 cM. There are 21 candidate genes in this region. It was finally found that the insertion mutation of Mu transposon occurred at 30 bp upstream of the coding region of zm00001d042618 (zmm16) by transponson tagging and sequencing analysis. The results showed that mi-ms-3 was a new allele of sts1, which caused by a single base mutation in the coding region. RT-PCR analysis indicated that the expression of zmm16 in the mutant was decreased. The identification of the new allelic mutant of sts1 in this study would provide new materials for the study of flower development and hybrid seed production.     

温度和湿度对伴性赤蚁sch系统致死性的影响

为进一步探讨家蚕伴性赤蚁(sch)致死的机理,比较了不同温度、湿度催青条件下,sch系统及其杂交F_1代和对照种黑蚁系统的孵化率、失水率、含水率、失水临界温度等。结果表明:伴性赤蚁sch系统在已_3至已_4期对温湿度敏感,受到高温和干燥的冲击,将获得“孵化不能”性状;温度、湿度因子对赤蚁sch孵化抑制均有较大的独立作用,sch表现出一定的“温度敏感性”和“湿度敏感性”;温度、湿度都能通过影响胚胎体内水分平衡失调而引起这种孵化不能;在不引起水分变化的情况下,温度还存在一条独立的抑制赤蚁孵化的途径。     

Morphological relationship between intercalated duct and pancreatic islet in streptozotocin and/or camostat mesilate administrations in the chicken

Present electron microscopical and immunocytochemistrical studies elucidated some morphological relationship between intercalated duct (ICD) and pancreatic islet cells in the chicken in streptozotocin (STZ) and/or camostat mesilate (CM) administrations. Twenty-one chickens were set into four experimental groups: (1) control group, (2) STZ administration group, (3) CM administration group, and (4) STZ + CM administration group. Cytoplasms of ICD cells stained more strongly with eosin in STZ administration group than other groups, and electron-dense materials and intercalated processes between ICD and islet cells were also increasing in time dependence in STZ administration. Number of pancreatic islet in STZ + CM co-administration was about 3.1 times larger than other groups. Many small sized cells were detected at surrounding area of ICD and they incorporated 5-bromo-2'-deoxyuridine better than other experimental groups. Present morphological data suggested that ICD cells might support some tolerances of pancreatic endocrine cells against toxic substances and also involve in regeneration of new pancreatic islet cells in STZ + CM co-administration.     

茶秆竹高生长模型的比较及组合选择

通过对 5种 S增长模型的比较及组合选择 ,结果表明 :S增长模型都具有单调性、渐近性和拐点 ,此特征能客观地反映诸多自然和经济规律 .通过一定的加权组合 ,用多个增长模型来反映 S变化过程 ,可以提高拟合的精度 ,多个模型的组合要优于单个模型 .用加权模型可以很好地反映茶秆竹的高生长过程 ,在实际生产中可选择 yp2 或 yp 3 组合     

家禽抑制素和活化素结构与功能的研究进展

许多研究显示,利用活化素和各种来源的抑制素作免疫原,可不同程度地提高家禽的排卵率和产蛋率.因此,研究家禽的这2种激素,对提高家禽产蛋量和繁殖性能具有重要的现实意义.文章对家禽抑制素和活化素的来源、结构特性及其对家禽生产性能的作用进行了综述.     

犬常见寄生虫病及防治

犬寄生虫病是犬病临床的常见病之一。犬患寄生虫病的种类很多,如果不能及时发现犬患上了寄生虫病或者未能及时准确地给予治疗,采取相应的防治措施,将会严重损害犬的健康。有些犬患寄生虫病还可以传染给人类,形成人犬共患危害人类身体健康。犬常见的寄生虫病有:蛔虫病、绦虫病、犬血丝虫病、     

新扬州鸡早期增重的OPAY02-SCAR分子标记研究

OPAY02型2条多态性条带经克隆、测序和引物设计后,转换成SCAR标记,并对86个新扬州鸡随机交配后代基因组DNA进行了PCR扩增.2条带DNA序列与红色原鸡基因组序列比对结果表明,大分子量条带与位于红色原鸡第3号染色体上序列有98%的同源性,共检测到8个SNPS,其中195位的碱基T→G,316位的A→T,538位的G→A,731位的T→A,1 147位的G→A,1 329位的T→C,1 927位的C→T,2 081位的C→T,小分子量条带与红色原鸡没有同源序列,推测新扬州鸡野祖除红色原鸡外,还有其它来源.SCAR标记分析表明,经条件优化随机扩增的OPAY02型标记稳定、可靠,可用于遗传分析.2条带所在座位群体基因型平衡性测验结果表明,所测新扬州鸡群体处于平衡状态,选择可以打破平衡,有利于动物育种.     

鲁西黄牛体细胞克隆保种技术的研究

以体外培养的鲁西黄牛耳皮肤成纤维细胞作核供体，研究利用体细胞克隆技术保存我国地方黄牛优良品种的技术方法。通过组织块培养法建立的鲁西黄牛（1♂，4♀）成纤维细胞系，作为核移植供体细胞，利用屠宰场母牛卵巢卵母细胞经体外培养成熟后作为核受体进行核移植，试验结果表明：重构胚的融合率为62．5％（242／387），分裂率为63．6％（154／242），体外培养第7天囊胚发育率为42．9％（66／154）。体外培养第7天的囊胚的内细胞团细胞（ICM）与滋养层细胞数平均为37和47，ICM占44．2％。体外发育到7d的囊胚新鲜胚胎的移植妊娠率（新鲜胚胎）移植受体10头，60d妊娠率为20％（2／10）。     

基因芯片技术及其在微生物检测中的应用

基因芯片技术是20世纪90年代发展起来的一门高新技术。应用该技术可以对大量的遗传信息进行快速、高通量、并行检测,解决了传统检测方法中遇到的难题,已广泛应用于基因表达分析、突变检测、核酸多态性分析、基因测序和药物筛选等几乎所有的生物学研究领域。作为一种高通量的基因检测方法,基因芯片技术在微生物检测和鉴定方面的应用越来越多,具有巨大的应用潜力。文章简要介绍了基因芯片技术的基本概念和技术流程,综述其在微生物检测和鉴定中的应用。