Genotype evaluation,muscle histopathology and ultrastructure in a Duchenne muscular dystrophy animal model

AIM: To evaluate the genotype , muscle histopathology and ultrastructure in dko mice. METHODS: Dystrophin/Utrophin-deficient double knockouts (dko) mice were obtained from university of Oxford, UK. Genotype of filial generation of heterozygote was evaluated by PCR-SSP. HE staining and fluorescent immunohistochemistry by SABC-Cy3 were used to detect striated muscle of dko mouse, and the muscle ultrastructure was observed by transmission electron microscope(TEM). RESULTS: In 112 filial generation mice, there were 28 mdx (25.0%), 26 dko (23.2%) and 58 heterozygote (51.8%), which coincided with the law of Mendelian inheritance. HE staining showed that the myocytes were not very uniform, there were phenomenon of round outline, centrally nucleated fibers, widening interspace, inflammatory cell infiltration and connective tissue proliferation in dko mice. There were no any immunofluorescent expression of dystrophin and utrophin in sarcolemma in dko mice. TEM showed sarcolemma breakage, separation and edema, and loose myofibril texture, inflammatory cell infiltration and connective tissue proliferation in dko mice. CONCLUSION: PCR-SSP is a very quick and accurate way for genotype evaluation of filial generation. The pathophysiology of dko mouse was very similar to Duchenne muscular dystrophy (DMD), and dko mouse is an ideal animal model for study of DMD clinical therapy.     

中国上市公司多元化经营绩效的实证分析

通过随机抽样,计算我国57家上市公司1998年-2002年的平均盈利指标和风险指标,根据各公司多元化熵值的大小对样本总体进行分组,分析多元化程度对企业绩效的影响.研究发现1.多元化程度越高,公司的平均净资产收益率越高;2.多元化经营降低了公司收益率的波动水平;3.资产规模越小的公司越倾向于选择多元化经营.     

槟榔红色素的抗氧化活性

测定槟榔红色素(areca red pigment,AP)对 DPPH·和·OH的清除能力、对脂质体过氧化的抑制能力、对Fe2+的络合能力,并测定其还原能力.结果表明:槟榔色素对DPPH·、·OH的清除能力较强:对脂质体过氧化的抑制能力很强,抑制率高达79.84%;与VE、没食子酸(gallic acid,GA)、BHT相比,槟榔色素的还原力、对Fe2+的络合能力较弱.说明槟榔红色素是较好的抗氧化剂.     

High frequency embryogenic callus induction and plant regeneration from mature caryposis of big bluestem and little bluestem

Big bluestem (Andropogon gerardii Vitman) and little bluestem [Schizachyrium scoparium (Michaux) Nash.] are native to the North America and are important forage grasses and ornamental grasses. Both grasses are proposed as ideal biomass producers for cellulosic ethanol production. To apply genetic transformation, which is an important tool for incorporating desirable agronomic traits into plants to both species, however requires an efficient and reproducible regeneration protocol. We used mature caryopses from big and little bluestem as explants and tested the effect of various combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) (1, 2, 3, 4 or 5 mg l⁻¹) and kinetin (KT) (0, 0.1 or 0.2 mg l⁻¹) on embryogenic callus induction with LS as the basal medium. The highest percentage of embryogenic calli induction occurred on medium containing 2, 4-D alone at 2 mg l⁻¹ for ‘Bison’ and on medium containing 4 mg l⁻¹ 2, 4-D alone for ‘Bonilla’ big bluestem. For little bluestem, the highest percentage of embryogenic callus induction occurred on medium containing 3 mg l⁻¹ 2, 4-D plus 0.1 mg l⁻¹ kinetin, suggesting that addition of KT is beneficial. Shoot regeneration took place on LS basal medium without any plant growth regulator for both species, although the addition of KT increased both regeneration frequency and the number of shoots produced per callus. Rooting of shoots reaching about 2 cm long occurred readily with or without α-naphthaleneacetic acid (NAA). Rooted plantlets were all successfully established in the soil.     

中国学术文献总库(CNKI)的主要功能和使用技巧

结合在线咨询服务和用户培训中积累的经验,就升级后新版的CNKI的主要功能和使用技巧进行了整理和总结.     

某土木工程试验班课程主线改进方案

从师生自身反馈角度，结合成立于2003年的中国地质大学工程学院土木工程试验班的具体情况，针对其本硕贯通式的培养教学计划，提出了包括增加专业软件学习、互动式外语教学、高低阶力学课程合并、压缩人文社科类课程等具体改进建议。     

优质高产紫花苜蓿新品种CW 400

CW 400系宁夏固原市农科所2000年从美国西海岸种子公司引入,通过多年多点试验、鉴定,2006年经宁夏回族自治区农作物品种审定委员会审定推广,适宜干旱半干旱地区种植.     

精梳棉卷成卷过程中起泡问题分析

介绍了成卷工艺对棉卷结构的影响以及棉卷成卷过程中起泡现象与准备工艺的关系.分析了棉卷成卷工艺过程中起泡过程和起泡原因,提出了防止起泡的措施.     

地形在园林中的设计原则及综合应用

介绍了园林地形的类型，阐述了地形在园林设计中的重要性及原则，提出了地形在园林设计中的综合应用。