小型摘穗收获机的研制

运用摘穗收获原理，研制了米麦套作地区小麦收获用的小型摘穗收获机，可一次完成田间站秆摘穗脱粒、粒穗分离、复脱、清选和装袋等工序。小型摘穗收获机与传统收获机相比。具有结构简单、工作可靠等特点。     

虚拟仪器技术在发动机故障诊断中的应用

为了达到对发动机运行状态进行实时监测和故障诊断的目的,采用PCI-9113A数据采集板实时采集发动机的技术状态参数,以图形化编程语言Lab VIEW实现数据的处理及管理,建立了发动机状态监测及故障诊断系统,实现了发动机振动信号的采集以及数据处理、显示、打印、存储等操作.以该系统为基础,只需添加一些所需要的传感器,同时增加相应的软件模块,就可实现对发动机其它性能参数的监测及故障诊断.     

叉车轮胎的非正常磨损及预防

叉车的使用因素、行车机构技术状况、驾驶技术等影响叉车轮胎的非正常磨损，应通过加强轮胎维护、提高底盘维护质量、合理选择和搭配轮胎等加以预防。     

灌溉试验站的总体规划和观测方法

随着农业产业结构调整的不断深入，传统的灌溉方式已不相适应。为了实现农业用水的高效利用,必须要有科学的灌溉试验成果作依据，做好灌溉试验站的规划建设和观测工作，为真正实现农业用水“总量控制、定额管理”奠定基础。     

任意形状均质地基二维渗流的可视

为了能够非常迅速和方便地分析网络生成质量和计算结果本身，使渗流分析过程简化，利用Matlab操作平台，将几何数据与数学模型参数、网格生成、渗流计算以及计算结果显示一体化，建立了具有GUI的任意形状地基渗流计算模型。介绍了网格生成方法、平面渗流的数学模型、渗流计算可视化编程、计算与实验的对比。     

洞庭湖区农机装备结构调整研究

农业机械是农业生产的先进手段和工具,其水平是农村生产力发展水平的重要标志之一。要实现传统农业向现代农业的转变,就必须用先进的农机装备武装农业。为此,从分析洞庭湖区农机装备结构入手,论证了其调整的可行性,预测了其发展的趋势,提出了调整的指导思想、目标、内容及具体措施,对当地调整农机装备结构具有一定的指导与参考作用。     

推动我国电力行业的发展措施探讨

能源是发展国民经济和提高人民生活水平的重要物质基础,作为能源工业的重要组成部分,电力行业的发展程度已成为衡量一个国家(地区)经济和社会发达程度的重要标志.改革开放以来,中国电力行业持续发展,取得了巨大的成就,但目前仍然存在一些问题,亟待解决。首先从电力企业的行业特点分析入手，对发电、输电、配电领域垄断程度及其原因进行了阐述，为当前电力体制的改革提供了理论依据，继而在对电力行业的发展现状及存在的问题分析基础上，提出了电力行业的发展措施。     

论农业机械化在新农村建设中的重要作用

以新农村建设带给农机化新的发展机遇为主线,结合武汉市以实施农村"家园建设行动计划"为抓手,建设社会主义新农村的实际,论述农业机械化在新农村建设中发挥的重要作用。     

旋转式开沟机工作部件的运动分析

对旋转式开沟机的工作部件进行了运动分析，探讨了机具的工作原理，参数设计，开沟铲尖的运动轨迹，沟的几何参数及开沟铲的运动参数，为机具的设计提供了理论依据。     

Construction of mouse ESC line stably expressing Pdx1 by Tet-On system

AIM To construct the mouse embryonic stem cell （ESC） line with stable pancreatic and duodenal homeobox 1 （Pdx1） expression by Tet-On system, which may lay a foundation for further research on the differentiation of Pdx1⁺ definitive endoderm cells into pancreatic cells. METHODS The Pdx1-overexpressing lentiviral vector with green fluorescent protein marker and puromycin resistance was constructed by Tet-On system and was used to infect the mouse ESC. The cells were divided into 3 groups： blank control group （ESC group）, empty lentivirus control group （PDX1^- ESC group） and Pdx1 lentivirus transfection group （PDX1⁺ ESC group）. Flow cytometry was used to detect the transfected cells after screening by doxycycline （DOX）. The function of Tet-On system and the expression of Pdx1 gene were detected. The transfected cells in PDX1^- ESC group and PDX1⁺ ESC group were sorted by flow cytometry, and constructed ESC line with stable expression of Pdx1 and negative control ESC line were verified. RESULTS （1） The positive rates of transfected cells in PDX1^- ESC group and PDX1⁺ ESC group were 90.72% and 94.01% after screening by DOX, respectively. The positive rates of transfected cells in PDX1^- ESC group and PDX1⁺ ESC group was 97.84% and 98.13% after sorting by flow cytometry, respectively. （2） With DOX, green fluorescence was observed in PDX1^- ESC group and PDX1⁺ ESC group. The mRNA and protein expression of Pdx1 was significantly increased in PDX1⁺ ESC group （P<0.05）. Without DOX, no green fluorescence was observed in the cells of the 3 groups, and no significant difference in the mRNA and protein expression of Pdx1 was observed （P>0.05）. （3） After 3 months of cryopreservation, the cell lines still survived in resuscitation culture and were regulated by DOX. CONCLUSION Using Tet-On system, the mouse ESC line with inducible Pdx1 expression were successfully established and could be used as an effective cell model to research the differentiation of Pdx1⁺ definitive endoderm cells into pancreatic cells.