Prediction of the surface roughness of wood for machining

The surface quality of solid wood is very important for its effective response in manufacturing processes. The effects of feed rate, cutting depth and rake angle on surface roughness and power consumption were investigated and modeled. Neuro-fuzzy methodology was applied and shown that it could be useful, reliable and an effective tool for modeling the surface roughness of wood. Thus, the results of the present research can be successfully applied in the wood industry to reduce time, energy and high experimental costs.     

A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction

Exposure to β-N-methylamino-l-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimer’s disease and Parkinson’s disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D₃BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%–32%), implying that D₃BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery (<10%). Most BMAA was found in a trichloroacetic acid soluble, bound form and we recommend including this fraction during analysis.     

Ancient genetic bottleneck and Plio-Pleistocene climatic changes imprinted the phylobiogeography of European Black Pine populations

The historical changes in European Black Pine population size across the whole natural distribution in Europe and Asia Minor were analyzed facing the Plio-Pleistocene climatic fluctuations. Thirteen chloroplast SSRs and SNPs markers have been studied under the assumptions of “neutral evolution.” Populations and meta-populations had different histories of migration routes, and they were strongly affected by complex patterns of isolation, fragmentation, speciation, expansion (1.88–4.28 Ma), purification selection (2.09–21.41 Ma) and bottleneck (1.85–21.76 Ma). A significant number of populations (min. 29–41%) were in equilibrium for very long periods. Generally, the bottleneck revealed by chloroplast DNA is weaker than the bottleneck revealed by nuclear DNA. The N _e immediately after the bottleneck reaches between 1820 and 3640 individuals. Generally, the historical effective population sizes shrink significantly for the Tertiary period from 10–15 up to 2.5 Ma in Western Europe (by 82%), followed by Asia Minor (69%) and the Balkan Peninsula (28%), likely resulting from important climatic changes. The rates and frequencies of stepwise westwards migration waves have been not sufficient to prevent isolation between the meta-populations and to suppress “sympatric speciation.” The migration was weak for the Pliocene, but was maximal for the Pleistocene, and finally silent for the present interglacial period, namely the Holocene.     

Changes in soil carbon stock under the wheat-based cropping systems at Vojvodina province of Serbia

The aim of this study was to assess the changes in soil organic carbon (SOC) stock in relation to the carbon (C) input from nine wheat-based cropping systems and untilled grass. The SOC pool ranged from 32.1 to 49.4 Mg ha^?1 at 0–20 cm and from 94 to 171 Mg ha^?1 at 0–100 cm for the arable soil, while in untilled grassland, it was higher (54 and 185 Mg C ha^?1, respectively). SOC stock was observed to be lower at the unfertilized 2-year rotation and higher at the 4-year rotation with manure and mineral fertilization. The study showed a winter wheat yield decrease of 176.8 kg ha^?1 for a 1- Mg ha^?1 SOC stock change in the 0–20-cm soil depth. The estimated C input for SOC stock maintenance was from 266 to 340 g C m^?2 year^?1 for winter wheat and rotations, respectively. Additional C input did not increase the SOC pool, suggesting that arable plots had a limited ability to increase SOC. These results provide guidance for the selection of management practices to improve C sequestration.     

Effect of lairage time,behaviour and gender on stress and meat quality parameters in pigs

The objective of this study was to determine the effects of lairage time (short vs. long), behavior (aggressive vs. non‐aggressive) and gender (gilts, barrows and boars) on stress and meat quality parameters in pigs (n = 100). In the group with short lairage, aggressive behavior of gilts and boars was observed. At exsanguination, blood samples were collected and levels of blood lactate and plasma cortisol were determined. Post mortem pH value, temperature, drip loss, sensory and instrumental color and meat quality class were determined on the muscle Longissimus dorsi. Higher blood lactate concentration (P < 0.05) and skin blemish score (P <0.001), lower temperature (P < 0.05) and drip loss (P < 0.01), as well as darker, less red and yellow meat color (P < 0.001) were found after long lairage compared to short lairage. Gilts had higher plasma cortisol concentrations than barrows (P < 0.05), while barrows had lower skin blemish scores (P < 0.05) than gilts and boars after long lairage. The highest incidence of PSE (pale, soft and exudative) meat was observed in barrows (100.00%) and boars (91.67%) after short lairage, while the lowest incidence of PSE meat was found in barrows (28.89%) after long lairage. Aggressiveness affected meat temperature, as aggressive pigs had higher meat temperature (P < 0.05) than non‐aggressive pigs.     

The attraction of cerambycids and other xylophagous beetles, potential vectors of Bursaphelenchus xylophilus, to semio-chemicals in Slovenia

The attractiveness of different semio-chemicals to potential vectors of the phytoparasitic nematode Bursaphelenchus xylophilus was investigated in conifer forests in Slovenia. From 2007 to 2009, the presence of xylophagous beetles in Pinus nigra, P. sylvestris, P. halepensis, Picea abies and Abies alba stands was assessed at eight locations. Insects were collected at 1-month intervals during the growing season using four cross vane traps per location with a collecting container with propylene glycol and attractants (ethanol+??-pinene, Pheroprax? and Gallowit?). The trapped insects represented 24 families of the order Coleoptera, and we identified 94 species. The most numerous group was the weevil subfamily Scolytinae (76.55% of all insects collected), followed by the family Cerambycidae (8.12%), and the weevil subfamily Curculioninae (1.67%). With regard to species number, the most frequent wood-borers were Cerambycidae (24 taxa), Scolytinae (12 species) and Buprestidae (8 species). The most abundant species was Spondylis buprestoides, followed by Arhopalus rusticus, Monochamus galloprovincialis and Arhopalus ferus. At all locations, the largest catch of Cerambycidae occurred in July. The most effective attractant was ethanol+??-pinene, followed by Gallowit?; the least effective attractant was Pheroprax?. Among Monochamus species, M. galloprovincialis represented 17.54%, M. sutor 0.09% and M. sartor 0.04% of the long-horned beetles collected. Monochamus individuals were most numerous in the P. nigra stand and were attracted in the greatest numbers by Gallowit?, followed by ethanol+??-pinene. The cerambycid catch was highly correlated with the catch of non-target bark beetle predators (Cleridae, Staphylinidae, Histeridae, Trogositidae, Nitidulidae, Rhizophagidae) in the traps.     

Long-term effects of agronomic practices on the soil organic carbon sequestration in Chernozem

The study was based on data from selected long-term field trials established at the Experimental Fields of the Institute of Field and Vegetable Crops, Novi Sad (Serbia). The effect of tillage systems on SOC concentration and SOC stock was most pronounced at 0–10 cm depth. In a 0–40 cm soil layer, in a 7-year period, no-till (NT) sequestrated 863 kg SOC ha^?1 yr^?1 more compared to moldboard plow tillage (PT), while the effects of disc tillage (DT) and chisel tillage (CT) were not significantly different. Unfertilized three-crop rotation (CSW) compared to two-crop rotation (CW) enhanced SOC storage in a 0–30 cm soil layer by 151 kg C ha^?1 yr^?1 in a 56-year period. Within fertilized treatments, SOC concentration was highest under continuous corn (CC). Mineral fertilization (F) non-significantly increased the SOC stock compared to no fertilization in corn monoculture in a 32-year period. The incorporation of mineral fertilizers and harvest residues (F + HR) and mineral fertilizers and farmyard manure (F + FYM) sequestered 195 and 435 kg C ha^?1 yr^?1 more than the unfertilized plot, respectively, in a 0–30 cm soil layer, in a 35-year period. Irrigation did not significantly affect SOC sequestration.