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Journal of Plant Diseases and Protection - To have an opportunity for reliable detection of benyvirus Beet soil-borne mosaic virus (BSBMV) and its discrimination from Beet necrotic yellow vein... 相似文献
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V.W.?Fomitcheva J.?SchubertEmail author F.?Rabenstein A.?Habeku? 《European journal of plant pathology / European Foundation for Plant Pathology》2005,112(3):259-266
The genomic fragments of two open reading frames (ORFs) 1 and 2 of German and Canadian PAV isolates of Barley yellow dwarf virus (BYDV-PAV) were sequenced. Sequences only slightly differed from previously published sequences of this virus. Two polyclonal antisera against proteins encoded by ORFs 1 and 2 of a German ASL-1 isolate were developed using recombinant antigens expressed in E. coli as a fusion either to His6− or thioredoxin-tags. In Western blot analysis with total protein extracts from BYDV infected plants, antisera efficiently recognized the 99 kDa fusion protein expressed from ORF1 and ORF2 (P1–P2 protein). Later in infection the P1–P2 protein disappeared and two smaller proteins, revealing sizes of 39 and 60 kDa, could be detected. 相似文献
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Viktoria W. Fomitcheva Jörg Schubert Antje Habekuss Thomas Thieme 《Gesunde Pflanzen》2009,61(2):87-91
Potato virus Y (PVY) causes great economic losses in potato production world-wide. Concerning important it has replaced Potato leafroll virus (PLRV). Virus is transmitted by different aphid species in a non-persistent manner. During last years the developing of new effective methods for PVY monitoring, forecasting and detection in aphids is of increasing interest. Sensitive, rapid detection of virus in its natural vectors is of a great need to investigate the relationship between aphid migration and the spread of PVY. Simple diagnostic protocol for the detection of non-persistent Plum pox virus and semipersistent Citrus tristeza virus in aphids, proposed by Olmos et al. (2005) was probed to validate and estimate the efficiency of its applying for the detection of PVY in different aphid species too. 相似文献
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