全文获取类型
收费全文 | 408篇 |
免费 | 25篇 |
国内免费 | 1篇 |
专业分类
林业 | 25篇 |
农学 | 10篇 |
基础科学 | 1篇 |
66篇 | |
综合类 | 15篇 |
农作物 | 16篇 |
水产渔业 | 119篇 |
畜牧兽医 | 149篇 |
园艺 | 4篇 |
植物保护 | 29篇 |
出版年
2023年 | 1篇 |
2022年 | 6篇 |
2021年 | 12篇 |
2020年 | 13篇 |
2019年 | 12篇 |
2018年 | 9篇 |
2017年 | 11篇 |
2016年 | 12篇 |
2015年 | 9篇 |
2014年 | 26篇 |
2013年 | 34篇 |
2012年 | 18篇 |
2011年 | 24篇 |
2010年 | 23篇 |
2009年 | 17篇 |
2008年 | 22篇 |
2007年 | 18篇 |
2006年 | 23篇 |
2005年 | 12篇 |
2004年 | 20篇 |
2003年 | 26篇 |
2002年 | 6篇 |
2001年 | 30篇 |
2000年 | 12篇 |
1999年 | 4篇 |
1998年 | 6篇 |
1997年 | 4篇 |
1995年 | 2篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1978年 | 1篇 |
1974年 | 1篇 |
1972年 | 2篇 |
排序方式: 共有434条查询结果,搜索用时 0 毫秒
1.
Influence of death struggle on the structural changes in chub mackerel muscle during chilled storage
Masashi Ando Masao Joka Satoshi Mochizuki Koh-Ichi Satoh Yasuyuki Tsukamasa Yasuo Makinodan 《Fisheries Science》2001,67(4):744-751
ABSTRACT: Chub mackerel (34–35 cm, approximately 500 g), which were caught by fishing with a rod and line at the Bungo Channel, Oita prefecture, were rested overnight in a fish preserve and either killed by decapitation (control group) or allowed to struggle in air for 30 min (struggled group). Muscle samples were excised every 4 h, and measurements on breaking strength and histological observations were done for both groups. The breaking strength of muscle in the control group was significantly higher than that in the struggled group, whereby a decrease in breaking strength was delayed for 12 h compared to the struggled group. Light microscopy showed space extension among muscle cells in association with a decrease in breaking strength. Especially in the struggled group, the extended area was larger and the difference in area was significant at the time when breaking strength showed a significant difference. Using electron microscopy, the extended area showed cut and/or disappeared collagen fibrils. From these results, it was demonstrated that struggling to death promoted the degradation of collagen fibrils and the weakening of connective tissue and, resultantly, led to the faster softening of muscle of chub mackerel. 相似文献
2.
Osamu Yamato Hiroyuki Satoh Naoaki Matsuki Kenichiro Ono Masahiro Yamasaki Yoshimitsu Maede 《Journal of veterinary diagnostic investigation》2004,16(1):39-44
In the present study, laboratory techniques were used to diagnose canine GM2-gangliosidosis using blood and cerebrospinal fluid (CSF) that can be collected noninvasively from living individuals. Lysosomal acid beta-hexosaminidase (Hex) was measured spectrofluorometrically using 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide and 4-methylumbelliferyl 7-(6-sulfo-2-acetamido-2-deoxy-beta-D-glucopyranoside) as substrates. Main isoenzymes A and B of Hex in leukocytes were also analyzed using cellulose acetate membrane electrophoresis. GM2-ganglioside in CSF was detected and determined quantitatively by using thin-layer chromatography/enzyme-immunostaining method with anti-GM2-ganglioside antibody. In normal dogs, Hex activities could be determined in leukocytes, serum, and CSF and the total activities were markedly reduced in all the enzyme sources in a dog with Sandhoff disease. Electrophoresis of a leukocyte lysate from a normal dog showed that the Hex A and Hex B were not separated distinctively with formation of a broad band, whereas there were no bands in electrophoresis of a lysate from a dog with Sandhoff disease, showing a deficiency in the total enzyme activity. GM2-ganglioside could be detected and determined quantitatively in as little as 100 microl of canine CSE GM2-ganglioside in CSF in a dog with Sandhoff disease increased to 46 times the normal level. In conclusion, the methods in the present study are useful for diagnosis of canine GM2-gangliosidosis. These techniques enable definitive and early diagnosis of canine GM2-gangliosidosis even if tissues and organs cannot be obtained. 相似文献
3.
Pallab Kumer Sarker Shuichi Satoh Haruhisa Fukada & Toshiro Masumoto 《Aquaculture Research》2009,40(2):225-232
Non-faecal phosphorus (P) was determined for large yellowtail to estimate a minimum available P requirement (Experiment 1) and to justify inorganic P supplementation in a fish meal-based diet (Experiment 2). In Experiment 1, purified diets with incremental P concentrations were fed to yellowtail (mean weight 917 g) at a feeding rate of 1.5% of body weight. The peaks of non-faecal P excretion appeared 5–6 h after feeding in fish fed more than 4.5 g available P kg−1 dry diet. Broken-line analysis indicated that the minimum available P requirement was 4.4 g kg−1 dry diet. In Experiment 2, a purified diet (PR) containing 6.5 g available P kg−1 and a fish meal-based diet with (F1) and without (F0) additional phosphorus were fed to yellowtail (mean weight 1.1 kg) at 1.5% (PR) and 2% (F0 and F1) feeding rates respectively. There was no significant difference in P excretion between fish fed the F0 (5.5 g soluble P kg−1 dry diet) and the PR diet. However, significantly higher (34.5%) amounts of non-faecal P excretions (7.4 g soluble P kg−1 dry diet) were found in fish fed F1 compared with the F0 diet. This suggested that there was an excess of dietary P in the F1 diet and that supplementation is not needed in fish meal-based diets for large yellowtail. 相似文献
4.
5.
Rhea Joy Carton-Kawagoshi Bessie Joy Elle Valeriano Corre Jr. Shuichi Satoh Masahiro Notoya Daisuke Fujita 《Aquaculture International》2014,22(2):573-584
This study tested the feasibility of a low-cost seaweed biofiltration system for pond-based aquaculture through an indoor-integrated fish-seaweed culture experiment using weekly nutrient supply regime and different seaweed stocking densities. The culture experiment was conducted in glass aquaria that were stocked with Gracilariopsis bailiniae at 3 densities (low = 0.5 kg m?2, middle = 2 kg m?2 and high = 3.5 kg m?2) and provided either with effluents from intensive milkfish (Chanos chanos) culture or with effluent-free seawater (control) as nutrient source. Stocking density was used as a factor in optimizing nutrient availability for growth and nutrient removal under such low water exchange conditions. Our results showed that G. bailiniae cultivated in milkfish effluents had higher growth, biomass and nitrogen yields than those cultivated in effluent-free seawater. Among the different stocking densities tested, highest growth rate (1.03 % day?1) was obtained in the middle density. Increasing biomass and nitrogen yields were also obtained at this density until the end of the culture period. Poorer growth rates at low and high stocking densities were attributed to light limitation from phytoplankton and self-shading, respectively. Due to seaweed treatment, average outflow concentration of NH4 + was reduced from half of its pretreated level. This study showed that a weekly effluent supply at 2 kg m?2 seaweed stocking density can sustainably support the growth of G. bailiniae as long as the dissolved nutrients are present at high levels. 相似文献
6.
Yasuo Tomida Toshiya Suzuki Tsutomu Yamada Ryuji Asami Hirofumi Yaegashi Yasufumi Iryu Tsuguo Otake 《Fisheries Science》2014,80(2):273-280
We examined differences in otolith oxygen (δ18Ootolith) and carbon (δ13Cotolith) stable isotope ratios between hatchery and wild pink salmon fry. The δ18Ootolith values of hatchery and wild fry were ?7.7 ± 0.2 ‰ and ?8.3 ± 0.3 ‰ (1σ), respectively. This difference reflected differences in temperature conditions experienced by each fry. The δ13Cotolith values of hatchery and wild fry were ?19.2 ± 0.3 ‰ and ?11.1 ± 1.8 ‰ (1σ), respectively. The lower δ13Cotolith values of hatchery fry were probably related to their intake of artificial diets. Discriminant analysis of δ18Ootolith and δ13Cotolith values demonstrated a highly significant difference between hatchery and wild fry with 95.8 % classification accuracy. Therefore, analysis of δ18Ootolith and δ13Cotolith precipitated in the fry stage may be useful for discriminating the origin of returning adult pink salmon. The discrimination method for returning adult fish would provide important information for evaluating the effect of hatchery release and their impact on the wild population. 相似文献
7.
Hirofumi Ido Hirofumi Nagao Hideo Kato Atsushi Miyatake Yasushi Hiramatsu 《Journal of Wood Science》2010,56(5):422-428
Tests of compression perpendicular to the grain were carried out on laminated veneer lumber (LVL) and timber. The species
tested were sugi, radiata pine, karamatsu, akamatsu, and dahurian larch; two sets of sugi specimens were tested, with the
sugi LVL products being manufactured in different plants. The strength properties of the materials for different loading directions
were compared for LVL and timber. At 5% compressive strain in the same materials, the average stress in the tangential direction
of timber was larger than that in the radial direction for all species except for radiata pine, and the average stress in
the edge-wise direction of LVL was larger than that in the flat-wise direction for all species except for radiata pine. When
the stress at 5% strain was compared in the same direction, the average stress of LVL in the edge-wise direction was larger
than that in timber in the tangential direction for all species, but there were no great differences between the average stress
of LVL in the fl at-wise direction and that of timber in the radial direction for all species except for radiation pine. There
was a close relationship between density and stress at 5% strain in LVL, especially in the edge-wise direction. For all results,
radiata pine did not follow the trend of the other species; The large annual ring width of radiata pine was considered to
have affected the results. 相似文献
8.
Genetic analysis for sow stayability at different parities in purebred Landrace and Large White pigs
Shinichiro Ogawa Makoto Kimata Kazuo Ishii Yoshinobu Uemoto Masahiro Satoh 《Animal Science Journal》2021,92(1):e13599
Genetic parameters for sow stayability were estimated from farrowing records of 10,295 Landrace sows and 8192 Large White sows. The record for sow stayability from parity k to parity k + 1 (k = 1, …, 6) was 0 when a sow had a farrowing record at parity k but not at parity k + 1, and 1 when a sow had both records. Heritability was estimated by using single-trait linear and threshold animal models. Genetic correlations among parities were estimated by using two-trait linear–linear and single-trait random regression linear animal models. Genetic correlations with litter traits at birth were estimated by using a two-trait linear–linear animal model. Heritability estimates by linear model analysis were low (0.065–0.119 in Landrace & 0.061–0.157 in Large White); those by threshold model analysis were higher (0.136–0.200 & 0.110–0.283). Genetic correlations among parities differed between breeds and models. Genetic correlation between sow stayability and number born alive was positive in many cases, implying that selection for number born alive does not reduce sow stayability. The results seem to be affected by decisions on culling made by farmers. 相似文献
9.
Kazuo Satoh Tomohiro Tanaka Yuuichi Oguro Reiji Takahashi Tatsuaki Tokuyama 《Soil Science and Plant Nutrition》2013,59(5):777-781
Long-term preservation of ammonia-oxidizing bacteria had been achieved until now by using the silica gel-freezing method. Recently, the freeze-drying method for the preservation of microorganisms has been widely used, and has been applied to the preservation of ammonia-oxidizing bacteria. The 10% skim milk-l% pyruvate or glutamate ratio was optimum as a dispersion-medium, and the preservation temperature of the freeze-dried cells was effective at -80°C. This method of preservation was found to be considerably more effective than the silica gel-freezing method. 相似文献
10.
Md. Shah Alam Sarker Shuichi Satoh Keisuke Kamata Yutaka Haga Yoshihiro Yamamoto 《Aquaculture Research》2012,43(4):538-545
A feeding experiment was conducted to investigate the effect of organic acids and/or lipid supplementation on growth, utilization and environmental loading of nitrogen (N) and phosphorus (P) in juvenile yellowtail fed fishmeal (FM) and plant protein (PP) diets. Six diets as FM (FM‐based), FM+P (FM with inorganic P), FM+L (FM with lipid), PP+CA (PP with citric acid), PP+L+CA (PP with lipid and citric acid) and PP+L+FA (PP with formic acid) were formulated. Yellowtails were fed each of the diets in duplicate groups; once a day, 6 days a week to near satiation at water temperature 19.0–25.0 °C for 16 weeks. Fishmeal with inorganic P gave the best growth while PP+L+FA the lowest. However, growth increased in PP+CA and PP+L+CA. Addition of lipid significantly increased N and P retention resulting in significant reduction in N and P excretion. Citric acid and FA supplementation to PP diets also increased retention of P; hence, its excretion was lowered. Thus, CA, FA and lipid in juvenile yellowtail diets can help to partially replace FM with PP sources and reduce inorganic P use to minimize environmental loading from aquafeeds. 相似文献