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1.
Yamana H Ito H Ito T Murase T Motoike K Wakabayashi K Otsuki K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(2):217-219
Heated and hydrated naturally occurring dolomite showed very strong antiviral activity. Infectivity of avian and human influenza, avian infectious bronchitis (coronavirus), Newcastle disease (paramyxovirus) and avian laryngotracheitis (herpesvirus) viruses dropped at least 1,000 fold following contact with the dolomite for five minutes at 4 degrees C. Dolomite is expected to be useful to inhibit the incidence of emerging and re-emerging infectious diseases. 相似文献
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3.
Imtiaj Hasan Shigeki Sugawara Yuki Fujii Yasuhiro Koide Daiki Terada Naoya Iimura Toshiyuki Fujiwara Keisuke G. Takahashi Nobuhiko Kojima Sultana Rajia Sarkar M. A. Kawsar Robert A. Kanaly Hideho Uchiyama Masahiro Hosono Yukiko Ogawa Hideaki Fujita Jiharu Hamako Taei Matsui Yasuhiro Ozeki 《Marine drugs》2015,13(12):7377-7389
MytiLec; a novel lectin isolated from the Mediterranean mussel (Mytilus galloprovincialis); shows strong binding affinity to globotriose (Gb3: Galα1-4Galβ1-4Glc). MytiLec revealed β-trefoil folding as also found in the ricin B-subunit type (R-type) lectin family, although the amino acid sequences were quite different. Classification of R-type lectin family members therefore needs to be based on conformation as well as on primary structure. MytiLec specifically killed Burkitt''s lymphoma Ramos cells, which express Gb3. Fluorescein-labeling assay revealed that MytiLec was incorporated inside the cells. MytiLec treatment of Ramos cells resulted in activation of both classical MAPK/ extracellular signal-regulated kinase and extracellular signal-regulated kinase (MEK-ERK) and stress-activated (p38 kinase and JNK) Mitogen-activated protein kinases (MAPK) pathways. In the cells, MytiLec treatment triggered expression of tumor necrosis factor (TNF)-α (a ligand of death receptor-dependent apoptosis) and activation of mitochondria-controlling caspase-9 (initiator caspase) and caspase-3 (activator caspase). Experiments using the specific MEK inhibitor U0126 showed that MytiLec-induced phosphorylation of the MEK-ERK pathway up-regulated expression of the cyclin-dependent kinase inhibitor p21, leading to cell cycle arrest and TNF-α production. Activation of caspase-3 by MytiLec appeared to be regulated by multiple different pathways. Our findings, taken together, indicate that the novel R-type lectin MytiLec initiates programmed cell death of Burkitt’s lymphoma cells through multiple pathways (MAPK cascade, death receptor signaling; caspase activation) based on interaction of the lectin with Gb3-containing glycosphingolipid-enriched microdomains on the cell surface. 相似文献
4.
Effects of photoperiod on gonadotropin-releasing hormone levels in the brain and pituitary of underyearling male barfin flounder 总被引:2,自引:0,他引:2
Masafumi AMANO Takeshi YAMANOME Hideaki YAMADA Koichi OKUZAWA Kunio YAMAMORI 《Fisheries Science》2004,70(5):812-818
ABSTRACT: A pleuronectiform fish, the barfin flounder Verasper moseri , expresses three gonadotropin-releasing hormone (GnRH) forms in the brain: salmon GnRH (sGnRH), chicken GnRH-II (cGnRH-II) and seabream GnRH (sbGnRH). To clarify the effects of photoperiod on GnRH systems, changes in brain and pituitary GnRH peptide levels were examined using time-resolved fluoroimmunoassays. In experiment 1, 5-month-old male barfin flounder (mean total length 9.0 cm, body weight 11.0 g) were divided into short (8:16 h light : dark [L:D] cycle; lights on 08.00–16.00 hours) and long photoperiod (16:8 h L:D cycle; lights on 04.00–20.00 hours) groups in mid September and maintained until November under natural water temperature (19.3–15.2°C). Brain sGnRH concentrations were significantly higher in the 16:8 h L:D group than in the 8:16 h L:D group, whereas no significant differences were observed in total length, body weight, plasma testosterone concentration, brain cGnRH-II concentration and pituitary sbGnRH content. In experiment 2, 7-month-old male barfin flounder (mean total length 16.5 cm, body weight 76.8 g) were divided into short and long photoperiod groups in mid December and maintained until February under natural water temperature (12.5–6.6°C). Total length, body weight and condition factor were significantly greater in the 16:8 h L:D group than in the 8:16 h L:D group, whereas no significant differences were observed in plasma testosterone concentration and GnRH levels in the brain and pituitary. These results indicate that levels of sGnRH in barfin flounder are influenced by photoperiodic treatment dependent on water temperature and/or body size. 相似文献
5.
In Sendai Bay, stone flounder larvae settle and spend their juvenile period in either shallow exposed inshore nursery grounds or estuarine nursery grounds. The purpose of this study is to examine the relative contributions of these two kinds of nursery grounds to the flounder population using otolith strontium:calcium ratios. Stone flounder juveniles were collected from both nursery grounds, and one- and two-year-old flounder were caught deeper in Sendai Bay. Sr and Ca content in the otoliths were measured by electron probe micro analysis. The Sr:Ca ratios in the otolith section corresponding to the early postsettlement period ranged from 3.06 to 3.85 for the exposed inshore areas with stable low temperature and high salinity conditions, and from 3.81 to 5.32 in brackish estuaries with high temperature and low salinity conditions but with large diel and tidal cyclical fluctuations. Values from an estuarine site with stable salinity ranged from 3.58 to 4.15 overlapping with both the above ranges. Rearing experiments supported our inference that the high otolith Sr:Ca ratios of juveniles inhabiting estuarine nursery grounds are attributable to higher temperature and physiological stress caused by the large diel temperature and salinity fluctuations within the estuaries. Estimation of the Sr:Ca ratio of recruited fish using the otolith section formed while in the nursery area showed that at least 20 out of 42 individuals examined originated from estuarine nursery grounds. The present study indicates that estuaries play an important role as nursery grounds for stone flounder, producing about half of the stock in spite of the small and restricted area compared with the wide expanse of the exposed inshore area. 相似文献
6.
An air-injection press, which has holes punched in the heating plates, injects high-pressure air through the holes of one
plate into boards during press heating. The air-injection press can manufacture boards from high-moisture-content particles
by controlling blowouts of the boards. In this study, boards were manufactured from particles that had a moisture content
of 25% by using the air-injection press, which reduced the required pressing time. Boards manufactured by injecting air through
holes of 5 mm in diameter were of poor quality with a low internal bond strength of only 0.31 MPa. When the hole diameter
was reduced to 1 mm, the internal bond strength increased to 0.44 MPa. A high air-injection pressure of 0.55 MPa also resulted
in improved board properties over those for boards manufactured at lower pressures. This was probably because a large amount
of binder was released from boards through the 5-mm holes, together with water vapor, during air injection; the small-diameter
holes reduced the release of binder, resulting in better board properties. 相似文献
7.
Passive protection of dogs against clinical disease due to Canine parvovirus-2 by specific antibody from chicken egg yolk 总被引:4,自引:0,他引:4 下载免费PDF全文
Sa Van Nguyen Kouji Umeda Hideaki Yokoyama Yukinobu Tohya Yoshikatsu Kodama 《Canadian journal of veterinary research》2006,70(1):62-64
The protective effect of immunoglobulins derived from chicken egg yolk (IgY) against infection by Canine parvovirus 2 (CPV-2) was evaluated in 10 beagle dogs orally challenged with a strain of the virus. The 2-mo-old dogs were divided into 3 groups and treated with powders containing CPV-2 IgY or normal egg yolk for 7 d after the challenge. The 4 dogs receiving normal egg yolk (control group) demonstrated mild symptoms typical of CPV-2 infection, such as vomiting, diarrhea, and weight loss. No symptoms were observed by 16 d after challenge in the 3 dogs receiving 2 g of IgY powder. Of the 3 dogs receiving 0.5 g of IgY powder, 2 had clinical CPV-2 disease; however, the manifestations were less severe than in the control group. Furthermore, the IgY-treated groups had significantly greater weight gain and shorter duration of virus shedding than the control group. These results indicate that IgY is useful in protecting dogs from CPV-2-induced clinical disease. 相似文献
8.
Mamoru Satou Kazufumi Nishi Masaharu Kubota Michiko Fukami Hideaki Tsuji Kees van Ettekoven 《Journal of General Plant Pathology》2006,72(3):193-194
The races for the causal agent of spinach downy mildew Peronospora farinosa f. sp. spinaciae were identified by inoculation of race-differential cultivars. One isolate was identified as Pfs:5s and the others belonged
to a new race. This is the first report of race Pfs:5 and another new race in Japan. 相似文献
9.
Hideaki Endo Eiji Takahashi Masataka Murata Hitoshi Ohnuki Huifeng Ren Wakako Tsugawa Koji Sode 《Fisheries Science》2010,76(4):687-694
A wireless biosensor system was developed for the continuous measurement of blood glucose levels in flatfish. The biosensor
was implanted in the interstitial fluid under the scleral surface of the eyeball (EISF) to investigate the relationship between
EISF and blood glucose levels. EISF glucose levels were found to be correlated with those in the blood and to be approximately
the same as blood glucose levels in the range of 7–25 mg dl−1. A needle-type biosensor was prepared for the continuous EISF glucose monitoring in flatfish. A working electrode was constructed
using platinum iridium wire, and glucose oxidase was immobilized to the electrode. The biosensor was inserted into the EISF
of flatfish for sensor implantation. A 650-mV potential (vs. Ag/AgCl) was applied by a wireless potentiostat to the working
electrode for the amperometric glucose measurement. We investigated whether glucose in the EISF can be determined in vivo.
The estimated glucose levels using a one-point calibration method were correlated with actual blood glucose levels. In conclusion,
using a wireless biosensor system, we were able to monitor blood glucose levels in flatfish under free-swimming conditions
for 16 h. 相似文献
10.
Satoshi Yamashita Tsutomu Hattori Shin Abe Hideaki Goto Hiroki Sato 《Journal of Forest Research》2014,19(1):143-153
Improvement cutting, which decreases tree density by cutting and thinning while leaving logging residues, has been practiced on Okinawa Island since the 1970s and changes not only the vegetation composition but also the amount and composition of woody debris on the forest floor. To quantitatively compare the effect of improvement cutting with the effects of woody debris and vegetation on the community structure of aphyllophoraceous fungi, we conducted a field survey of vegetation composition, the amount and composition of woody debris, and the aphyllophoraceous fungi flora on Okinawa Island in December 2006. We established 3 plots in forest stands without logging and 13 plots in a forest that had undergone improvement cutting from 1 to 22 years before our field survey. The number and volume of woody debris tended to be highest soon after improvement cutting. We recorded 1,172 fungal samples belonging to 81 species in four orders. Fungal species richness was correlated with the composition of the woody debris. Fungal species composition in forest plots 1 year after improvement cutting differed from most of the other logged forests. Variation partitioning revealed that the effects of improvement cutting explained 14.1 % of the total variation in fungal species composition, and that differences in woody debris and vegetation not related to improvement cutting explained 40.4 %. Changes in the composition of woody debris caused by improvement cutting explained at most 2.7 % of the variation. This suggests that improvement cutting does not have a strong effect on fungal community structure, but woody debris does. 相似文献