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Sadamasa ISHIKAWA Kou HIRAGA Yuuki HIRADATE Kentaro TANEMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):725-728
Acetamiprid (ACE) and imidacroprid (IMI) are known neonicotinoid insecticides with strong
affinities for the insect-selective nicotinic acetylcholine receptor. These provide insect
control by hyperstimulating insect nerves and are used for agricultural pest management.
However, it has also been reported that ACE and IMI affect mammalian reproductive
function. We determined the effects of ACE and IMI on the in vitro
maturation of porcine oocytes. Significant decreases in nuclear maturation rates were
observed in the ACE or IMI-exposed groups. Also, in matured oocytes from the ACE or
IMI-exposed groups, irregular chromosomes were observed. Our results suggest that ACE and
IMI exposure was detrimental to porcine oocytes and the extent of the effects depends on
the concentration of exposure. 相似文献
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Hiromichi Mitamura Keiichi Uchida Yoshinori Miyamoto Nobuaki Arai Toshiharu Kakihara Takashi Yokota Junichi Okuyama Yuuki Kawabata Tohya Yasuda 《Fisheries Science》2009,75(5):1133-1140
Several species of fish exhibit strong homing ability and distinct site fidelity to their original habitats and spawning sites.
We have studied the homing ability, site fidelity, and diel movement patterns of three nocturnal black rockfish Sebastes inermis. The patterns were studied by radio-linked acoustic positioning system (VRAP) and fixed monitoring receivers (VR2) in Maizuru
Bay, Japan from June to July 2004. All tagged fish were displaced by 80–120 m, but they returned to their respective captured
sites at dusk, regardless of the time of release. The fish tagged with a depth sensor transmitter displayed active vertical
movement just after the release, and subsequently swam near the bottom depth around the captured site. After homing, they
displayed small home ranges [average 815 ± 265 m2 (SD)] around a single rocky area with one or two core areas (average 64 ± 30 m2). One core area was utilized during the daylight and dark periods, whereas the other was used primarily during the dark period.
The depth-tagged fish primarily used the depth of the rock crevice during the daylight, and it spent its dark period between
the surface and the deeper bottom depth. 相似文献
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Yuuki Kawabata Junichi Okuyama Hiromichi Mitamura Kimio Asami Kenzo Yoseda Nobuaki Arai 《Fisheries Science》2007,73(5):1147-1154
ABSTRACT: Post-release movement and diel activity patterns of hatchery-reared and wild black-spot tuskfish were examined using ultrasonic telemetry. Five hatchery-reared and four wild fish were released in the sandy bottom of Urasoko Bay, Ishigaki Island, Okinawa, Japan, and monitored using automated monitoring receivers from November 2005 to February 2006. Both hatchery-reared and wild fish tended to stay near the release site for over two weeks, before leaving the release site. Both hatchery-reared and wild tuskfish showed diurnal rhythm intermittently; signals were recorded more frequently in the daytime and less frequently in the nighttime, suggesting that the fish of both origins were active during the day and inactive during the night. These findings indicate that the one-year-old hatchery-reared tuskfish have some consistent behavioral characteristics with those of the wild. 相似文献
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Asagiri M Hirai T Kunigami T Kamano S Gober HJ Okamoto K Nishikawa K Latz E Golenbock DT Aoki K Ohya K Imai Y Morishita Y Miyazono K Kato S Saftig P Takayanagi H 《Science (New York, N.Y.)》2008,319(5863):624-627
Cathepsin K was originally identified as an osteoclast-specific lysosomal protease, the inhibitor of which has been considered might have therapeutic potential. We show that inhibition of cathepsin K could potently suppress autoimmune inflammation of the joints as well as osteoclastic bone resorption in autoimmune arthritis. Furthermore, cathepsin K-/- mice were resistant to experimental autoimmune encephalomyelitis. Pharmacological inhibition or targeted disruption of cathepsin K resulted in defective Toll-like receptor 9 signaling in dendritic cells in response to unmethylated CpG DNA, which in turn led to attenuated induction of T helper 17 cells, without affecting the antigen-presenting ability of dendritic cells. These results suggest that cathepsin K plays an important role in the immune system and may serve as a valid therapeutic target in autoimmune diseases. 相似文献
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Ushiro Y Hashimoto O Seki M Hachiya A Shoji H Hasegawa Y 《The Journal of reproduction and development》2006,52(4):487-495
Activins, TGF-beta superfamily members, have multiple functions in a variety of cells and tissues. Additional activin beta subunit genes, betaC and betaE, have been identified in humans and rodents. To explore the role of activin betaC subunit, we generated recombinant human activin C using Chinese hamster ovary cells. Recombinant activin C from the conditioned medium was purified by consecutive hydrophobic, size-exclusion, and high performance liquid chromatography. SDS-PAGE and Western blot analysis of the purified protein revealed that activin C formed disulfide bridges. However, activin C had no effect on the proliferation of cultured liver cells. Furthermore, there were no significant differences in erythroid differentiation and follicle stimulating hormone secretion in vitro. It was also shown that immunoreactive bands indicated the hetrodimer of activin betaC, and inhibin alpha subunits were detected in the conditioned medium from the activin C-producing cells, which were stably transfected with inhibin alpha subunit cDNA. This suggests that activin betaC subunit may have been present and that it may exert its effect as inhibin C. 相似文献
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Atsushi IDETA Yoshito AOYAGI Kanami TSUCHIYA Yuuki NAKAMURA Kou HAYAMA Atsushi SHIRASAWA Kenichiro SAKAGUCHI Naomi TOMINAGA Yoshiyuki NISHIMIYA Sakae TSUDA 《The Journal of reproduction and development》2015,61(1):1-6
Embryos obtained via superovulation are necessary for mammalian artificial reproduction, and viability is a key determinant of success. Nonfreezing storage at 4 C is possible, but currently used storage solutions can maintain embryo viability for only 24–48 h. Here we found that 10 mg/ml antifreeze protein (AFP) dissolved in culture medium 199 with 20% (v/v) fetal bovine serum and 25 mM HEPES could keep bovine embryos alive for 10 days at 4 C. We used a recombinant AFP isolated from the notched-fin eelpout (Zoarces elongatus Kner). Photomicroscopy indicated that the AFP–embryo interaction was enhanced at 37 C. Embryos pre-warmed with the AFP solution at 37 C for 60 min maintained high viability, whereas those that were not pre-warmed could live no longer than 7 days. Thus, short-term storage of bovine embryos was achieved by a combination of AFP-containing medium and controlled pre-warming. 相似文献
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Yoshiki SHIRAKATA Yuuki HIRADATE Hiroki INOUE Eimei SATO Kentaro TANEMURA 《The Journal of reproduction and development》2014,60(5):383-387
The core histone is composed of four proteins (H2A, H2B, H3 and H4). Investigation of the modification patterns of histones
is critical to understanding their roles in biological processes. Although histone modification is observed in multiple cells
and tissues, little is known about its function in spermatogenesis. We focused on the modification patterns of histone H4
during murine spermatogenesis. We demonstrated that the individual N-terminal sites of H4 show different modification
patterns during the differentiation of male germ cells. The methylation pattern varied depending on the residues that were
mono-, di-, or tri-methylated. All the H4 modifications were high during the meiotic prophase, suggesting that histone H4
modification plays an important role during this stage of spermatogenesis. Elongating spermatids showed increased acetylation
of histone H4, which may be associated with a histone-to-protamine substitution. Our results provide further insight into the
specific relationship between histone H4 modification and gene expression during spermatogenesis, which could help to
elucidate the epigenetic disorders underlying male infertility. 相似文献
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