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1.
Suhaila Mohamed Suzana Saka Saleh H. El-Sharkawy Abdul Manaf Ali Sepiah Muid 《Pest management science》1996,47(3):259-264
Ethanolic extracts of 58 Malaysian plants belonging to 24 different families were screened for antifungal activity against seven plant pathogens using the filter paper disc diffusion technique. Two varieties of Piper betle, showed strong activity against all the pathogens tested (Colletotrichum capsici, Fusarium pallidoroseum, Botryodiplodia theobromae, Alternaria alternata, Penicillium citrinum, Phomopsis caricae-papayae and Aspergillus niger), with inhibition diameters significantly (P<0·01) bigger than 2·5 mg ml−1 prochloraz or 10 mg ml−1 clotrimazole. The minimum inhibitory concentrations of the ethanolic extracts of P. betle against these plant pathogens ranged between 0·01 mg ml−1 and 1 mg ml−1. Thirty-four other plants (Kucing gala, Limau batik, Bertholletia excelsa, Bixa orellana, Caesalpinia pulcherrima, Cerbera odollam (fruits and leaves), Colocasia gigantea, Curcuma domestica, Curcuma manga, Derris eliptica, Elephantopus scaber, Eleusine indica, Eugenia polyantha, Euphorbia hirta, Euphorbia tirucalli, Gardenia florida, Hedyotis auricularia, Hibiscus rosa-sinensis, Juniperus chinensis (three varieties), Lawsonia inermis, Lecythis ollaria, Mentha arvensis, Mimusops elengi, Ocimum sanctum, Phyllanthus niruri, Piper nigrum, Piperomia pellucida, Pedilanthus tithymaloides, Polygonum minus, Spondias dulcis, Solanum nigrum, Tinospora tuberculata) showed selective antifungal activity, while 21 species were inactive. 相似文献
2.
de Castro LA Rodrigues Pedroso T Kuchiishi SS Ramenzoni M Kich JD Zaha A Henning Vainstein M Bunselmeyer Ferreira H 《Veterinary microbiology》2006,116(4):258-269
The Mycoplasma hyopneumoniae genome contains at least 22 regions with a variable number of tandem nucleotide repeats (VNTRs) within coding DNA sequences (CDSs). In this work, the VNTR-containing CDSs were analysed in order to evaluate their degree of variation, possible correlations with antigenic properties, and their potential to be used as a basis for a strain typing PCR assay. We have analysed the VNTRs in five M. hyopneumoniae strains (J, 7448, 7422, PMS, and 232), based on published genomic sequences and on amplified and sequenced DNA segments. These VNTRs are distributed among 12 genes, most of which encode putative surface proteins, including known adhesins. The number of repeat units in any of the VNTRs is highly variable among the analysed strains, but they are, without exception, translationally in frame, and, therefore, code for a variable number of aminoacid repeats (VNTARs). These VNTARs determine putative structural, physicochemical and antigenic variations in the corresponding proteins, with potential implications for aspects associated to M. hyopneumoniae pathogenicity, such as cell adhesion and interactions with the host immune system. Considering that the characterized VNTARs are relatively stable, at least in vitro, and their sizes are strain-specific, we have developed a VNTR-based PCR assay for M. hyopneumoniae strain identification, useful for enzootic pneumonia (EP) diagnosis, strain typing, and distinction of circulating field isolates from vaccine strains in animals vaccinated against EP. 相似文献
3.
Agricultural practice in the semi-arid region of Brazil is highly dependant on irrigation. As access to water is limited in
the region, there is a need to guarantee its efficient use, especially in small-scale farming schemes. Models adequately calibrated
for semi-arid conditions and for typical crops are useful tools for analysis of on-farm strategies to improve water use efficiency.
A physically based agrohydrological model, SALTMED, is calibrated and validated for carrots (Daucus carota L., Brasília variety) and cabbage (Brassica oleracea var. capitata), two of the main crops in small-scale irrigated agriculture in the northeast of Brazil. SALTMED is also calibrated for castor
beans (Ricinus communis L.) under rainfed conditions. The results demonstrated the suitability of the SALTMED model for simulating soil water dynamics
and crop yield in the area. Predicted time series of soil water content and matric potential of root zone showed good agreement
with observed values, as shown graphically and statistically. Using the calibrated and validated model, management scenarios
were analysed in terms of applied irrigation water, irrigation frequency, soil and crop types. Impact of climate change on
the irrigation water requirements was also briefly analysed for possible changes in annual temperature using two different
emission scenarios. Analysis of possible impact of climate change on temperature related to two emission scenarios on the
area showed an increase around 11% of the crop water requirement for carrots and cabbage, for the low emission scenario, and
around 17% for the high emission scenario. The analysis of management scenarios indicated possible over-irrigation in the
area. The simulation showed that the deficit irrigation was a useful water-saving strategy for the region. The simulations
also indicated that irrigation frequency affected crop water use and differed according to the soil type. 相似文献
4.
C Voiculescu Lucia Stanciu Mihaela Voiculescu Suzana Rogoz I Dumitriu 《Comparative immunology, microbiology and infectious diseases》1983,6(4):291-299
By using CBA/J mice as responders, the immunodepressive effect of seven antibacterial chemotherapeutic agents was tested, i.e. penicillin (pen), streptomycin (str), erythromycin (erm), kanamycin (kan), tetracycline (tet), colistin (col) and chloramphenicol (chl). Lymphocytotoxicity power, as well as the ability of each drug to influence secondary humoral (against sheep red blood cells or diphtheria anatoxin) or cell-mediated (against PPD and Coxsackie A9 virus) immunity were searched.
Erm, col and chl markedly depressed humoral and cell-mediated immune responsiveness in vitro after in vivo treatment with non-cytotoxical amounts. The Th lymphocyte supplementation of B reactive spleen cell population recovered the immune capacity in col and chl in vivo-treated groups, but not in erm-treated group. 相似文献
5.
éverton Tenório de Souza Daysianne Pereira de Lira Aline Cavalcanti de Queiroz Diogo José Costa da Silva Anansa Bezerra de Aquino Eliane A. Campessato Mella Vitor Prates Lorenzo George Emmanuel C. de Miranda Jo?o Xavier de Araújo-Júnior Maria Célia de Oliveira Chaves José Maria Barbosa-Filho Petr?nio Filgueiras de Athayde-Filho Bárbara Viviana de Oliveira Santos Magna Suzana Alexandre-Moreira 《Marine drugs》2009,7(4):689-704
The antinociceptive and anti-inflammatory activity of caulerpin was investigated. This bisindole alkaloid was isolated from the lipoid extract of Caulerpa racemosa and its structure was identified by spectroscopic methods, including IR and NMR techniques. The pharmacological assays used were the writhing and the hot plate tests, the formalin-induced pain, the capsaicin-induced ear edema and the carrageenan-induced peritonitis. Caulerpin was given orally at a concentration of 100 μmol/kg. In the abdominal constriction test caulerpin showed reduction in the acetic acid-induced nociception at 0.0945 μmol (0.0103–1.0984) and for dypirone it was 0.0426 μmol (0.0092–0.1972). In the hot plate test in vivo the inhibition of nociception by caulerpin (100 μmol/kg, p.o.) was also favorable. This result suggests that this compound exhibits a central activity, without changing the motor activity (seen in the rotarod test). Caulerpin (100 μmol/kg, p.o.) reduced the formalin effects in both phases by 35.4% and 45.6%, respectively. The possible anti-inflammatory activity observed in the second phase in the formalin test of caulerpin (100 μmol/kg, p.o.) was confirmed on the capsaicin-induced ear edema model, where an inhibition of 55.8% was presented. Indeed, it was also observed in the carrageenan-induced peritonitis that caulerpin (100 μmol/kg, p.o.) exhibited anti-inflammatory activity, reducing significantly the number of recruit cells by 48.3%. Pharmacological studies are continuing in order to characterize the mechanism(s) responsible for the antinociceptive and anti-inflammatory actions and also to identify other active principles present in Caulerpa racemosa. 相似文献
6.
Luiz Henrique Agra Cavalcante-Silva Ana Carolina de Carvalho Correia José Maria Barbosa-Filho Bagnólia Araújo da Silva Bárbara Viviana de Oliveira Santos Daysianne Pereira de Lira Jéssica Celestino Ferreira Sousa George Emmanuel C. de Miranda Fabiana de Andrade Cavalcante Magna Suzana Alexandre-Moreira 《Marine drugs》2013,11(5):1553-1564
In this work, we investigated the spasmolytic effect of caulerpine, a bisindole alkaloid isolated from marine algae of the Caulerpa genus, on guinea pig ileum. Our findings indicated that caulerpine inhibited phasic contractions induced by carbachol (IC50 = 7.0 ± 1.9 × 10−5 M), histamine (IC50 = 1.3 ± 0.3 × 10−4 M) and serotonin (IC50 = 8.0 ± 1.4 × 10−5 M) in a non-selective manner. Furthermore, caulerpine concentration-dependently inhibited serotonin-induced cumulative contractions (pD′2 = 4.48 ± 0.08), shifting the curves to the right with Emax reduction and slope of 2.44 ± 0.21, suggesting a noncompetitive antagonism pseudo-irreversible. The alkaloid also relaxed the ileum pre-contracted by KCl (EC50 = 9.0 ± 0.9 × 10−5 M) and carbachol (EC50 = 4.6 ± 0.7 × 10−5 M) in a concentration-dependent manner. This effect was probably due to inhibition of Ca2+ influx through voltage-gated calcium channels (CaV), since caulerpine slightly inhibited the CaCl2-induced contractions in depolarizing medium without Ca2+, shifting the curves to the right and with Emax reduction. According to these results, the spasmolytic effect of caulerpine on guinea pig ileum seems to involve inhibition of Ca2+ influx through CaV. However, other mechanisms are not discarded. 相似文献
7.
An attempt at implementing a holistic inclusive development model: Insights from Malaysia's land settlement scheme 下载免费PDF全文
Mohd Zufri Mamat Boon‐Kwee Ng Suzana Ariff Azizan Lee Wei Chang 《Asia Pacific viewpoint》2016,57(1):106-120
This paper examines the influences of Malaysia's Federation Land Development Authority (FELDA) scheme in fostering inclusive rural development. Based on the model of holistic inclusive development, the paper investigates the performance of FELDA from four perspectives: social development, industrialisation, modernisation and basic needs. The main findings of the study indicate that, to a large extent, the scheme has successfully stimulated both the social and economic development of the community. Nonetheless, establishing a strong trust, social cohesion and rapport between the public authorities and community remain the main challenges in determining the success of this state‐led agenda. Moreover, keeping traditional values in the modern system will be the key principle for the sustainability of the programme if plans are made to adopt the scheme in other regions. 相似文献
8.
Reaction kinetics of fixation of CCA-C (chromated copper arsenate type C) preservative was studied at 30°C in ground wood
of trembling aspen, red pine, and red maple at treatment retentions of 4.0, 6.4, 9.6, and 30 kg/m3, and red maple pre-extracted with hot water at retentions of 6.4 and 30 kg/m3. Reaction orders of cumulative Cr, Cu, and As reactions decreased gradually during the fixation if calculated by Van’t Hoff’s
method. With Essen’s method, CCA fixation was best approximated as follows: Cr—3rd order reaction during the first 24 h, and
1st order reaction for the rest of the fixation period; Cu—2nd order reaction; and As—1st order reaction in red pine and aspen,
and 2nd order in red maple. Rates of reaction decreased with increased CCA solution concentration for Cr and Cu, and increased
for As, except in red maple. Reaction rates for all CCA elements were significantly higher in rapidly fixing red maple than
in regularly fixing red pine and aspen, and were higher in unextracted than pre-extracted red maple. Modeling of CCA fixation
kinetic for the whole fixation period enabled comparison of fixation reactions among wood species, preservative components,
and treatment retentions. 相似文献
9.
Practical diets with essential oils of plants activate the complement system and alter the intestinal morphology of Nile tilapia 下载免费PDF全文
Gustavo M. R. Valladão Sílvia U. Gallani Gabriela Pala Raphael B. Jesus Suzana Kotzent Jaqueline C. Costa Thiago F. A. Silva Fabiana Pilarski 《Aquaculture Research》2017,48(11):5640-5649
The effect of the essential oils (EOs) of peppermint, Mentha piperita L., and tea tree, Melaleuca alternifolia (Maiden & Betche) Cheel, on the haematological, biochemical, and immunological parameters and intestinal morphology of Nile tilapia, Oreochromis niloticus L., was evaluated. Fish (58.09 ± 5.87 g) were fed 100 mg/kg and 250 mg/kg of each EO and sampled on days 7, 14, 30 and 60 after starting supplementation. The haematological and biochemical parameters were not altered by the supplementation of EOs compared to the control (p > .05). With regard to the immunological parameters, the activation of the complement system of fish fed 250 mg/kg peppermint and 100 mg/kg and 250 mg/kg tea tree EOs were significantly higher compared to the control after 60 days of feeding (p < .05). The complement system plays an essential role in innate immunity and contributes significantly to the acquired immune response; thus, its activation through supplementation with EOs is promising for the formulation of nutritional additives in aquaculture. Regarding intestinal morphology, fish fed 250 mg/kg tea tree EO presented higher villus size compared to all other groups (p < .05), which represents a healthier gut. These fish present a larger intestinal surface, which can result in better absorption and utilization of the nutrients. Based on the responses found in this study, both EOs were considered promising for the formulation of feed additives for Nile tilapia. 相似文献
10.
Santos SC Costa WF Ribeiro JP Guimarães DO Ferri PH Ferreira HD Seraphin JC 《Fitoterapia》2002,73(4):292-299
A comparative study of tannin composition of three species known as barbatim?o (Stryphnodendron adstringens, Stryphnodendron polyphyllum and Dimorphandra mollis) was developed by TLC, hydrolysis followed by PC, and colorimetry. Principal component analysis of the data obtained showed strong differentiation between the genus Dimorphandra and Stryphnodendron. Additionally, barks from the two Stryphnodendron sp. also presented considerable differences in the tannin structure. 相似文献