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1.
Gene expression in black tiger prawns (Penaeus monodon) was studied following intra-muscular injection of CMVGal plasmid into the second abdominal segment. We used an in situ staining technique to detect -gal expression in one- and three-month-old injected prawns. We found that only one of the three-month-old prawns expressed the marker gene (2 days after injection), and the site of expression was confined to the sixth abdominal segment away from the injection site. We repeated the experiment on a new batch of three-month-old prawns, but using fluorometric determination technique. This time we found that -gal expression was detected (6/42) at the site of injection after 2, 7, and 14 days. In two other test samples, transgene expression was detected in the sixth abdominal segment only, further confirming the possibility of injected DNA dispersal. The results of the study also suggest that direct gene transfer is a feasible technique in black tiger prawns. 相似文献
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The present study was carried out using juveniles of silver pomfret, Pampus argenteus, produced in the hatchery by stripping eggs from spawners collected from the wild during 2004 and 2005 culture period. The experiments carried out during 2004 investigated the efficiency of using salmon feed mixed with cyclopeeze, salmon feed mixed with shrimp meat, salmon feed alone, and shrimp meat alone in the diet using 1 m3 capacity round fiberglass tanks in three replicates for each treatment with flow‐through seawater. The results showed that inclusion of shrimp meat in the diet either with salmon feed or alone give significantly high (P < 0.01) weight gain of up to 1.10 ± 0.06 g/fish/d and significantly lower (P < 0.001) feed conversion ratio compared to that of feeds without shrimp meat. The experiments carried out during 2005 investigated the use of commercially available feeds with feed additives. The results showed that feeding with “Gemma” feed with 54.0% crude protein and 19.0% crude fat or salmon feed with 41.4% crude protein and 23.9% crude fat give significantly high (P < 0.01) growth rates compared to that of pompano feed with 43.0% crude protein and 6.0% crude fat. The results of these investigations although show high growth rate of the fish compared to that of previous observations; there is yet an urgent need to formulate a suitable feed for this fish species for commercial ventures. 相似文献
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Wasiu A. Jimoh Mohd S. Kamarudin Muhammad A. Sulaiman Akeem B. Dauda 《Aquaculture Research》2019,50(11):3401-3411
The performance of selected leaf meals of high dietary fibre in the feed of a tropical commercial carp, hybrid lemon fin barb (Barbonymus gonionotus ♀ × Hypsibarbus wetmorei ♂) was evaluated in a 56‐day feeding trial. The tropical carp juveniles (9.43 ± 0.05 g) were randomly stocked in 60‐L aquaria at 15 fish per aquarium. Five isonitrogenous and isocaloric diets (30% crude protein, 17 kJ/g gross energy) containing no leaf meal and 10% napier grass (Pennisetum purpureum), alfalfa (Medicago sativa), water spinach (Ipomea aquatica) and Gliricidia sepium leaf meals were formulated and tested. Three aquaria were randomly assigned to each experimental diet. The results showed that fish‐fed diets containing leaf meals showed superior growth performance and body composition which was better than those fed control diet. The leaf meal‐treated groups also had higher values of protein, lipid, energy retention and production of digestive enzymes amylase. Conclusively, the results indicated that leaf meal fibre provided better performance showing the inherent prebiotic effect of the utilization of these leaf meal in hybrid lemon fin barb . 相似文献
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Anna A. Filatenko Karl Hammer Sulaiman Al Khanjari Andreas Buerkert 《Genetic Resources and Crop Evolution》2010,57(8):1135-1139
Due to its geographic position on the northeastern tip of the Arabian Peninsula and its sea trade relationships with Asia,
East Africa and the Middle East, Oman has for millennia been at the cross-roads of inter-regional exchange of cultivated plants.
This is reflected in recent findings of new cultivars of banana (Musa spp.) and wheat (Triticum spp.) in remote oases of the Hajar Mountains in northern Oman. Material collected in 2003 and 2004 contained six new botanical
varieties of wheat which are described here. One of them belongs to the tetraploid T. aethiopicum, the others are hexaploid. 相似文献
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【目的】研究红宝石无核×红旗特早葡萄杂交后代核性状遗传规律。【方法】以红宝石无核×红旗特早杂交群体135个F1代植株为试材,鉴定核状态,测定果实单粒重、单核重、单粒核数量、单核纵横径积等核相关指标,分析群体核性状遗传倾向。【结果】红宝石无核×红旗特早杂交群体胚败育率为59.26%;果实单粒重表现为连续分布,遗传力低,呈现变小趋势;果实单核重在有核和无核单株群体之间存在两极分化,但没有明显界限,遗传力低,呈现变小趋势;果实单粒核数量遗传力低,呈现变少趋势,整个组合91.85%的单株具有败育残核;单核纵横径积在有核和无核单株群体之间存在两极分化,且有明显界限,遗传力低,呈现变小趋势,但在有核单株中表现增大趋势。【结论】红宝石无核与具有无核倾向的有核品种红旗特早杂交,可以获得较高比例的无核后代,群体在果粒质量、核质量、数量及纵横径积等指标上均表现为退化趋势,但在无核单株与有核单株群体之间存在一定差异,果粒质量、核质量、数量及纵横径积与核性状正相关联。 相似文献
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Lamiaa A. Shaala Diaa T. A. Youssef Mansour Sulaiman Fathy A. Behery Ahmed I. Foudah Khalid A. El Sayed 《Marine drugs》2012,10(11):2492-2508
A new collection of several Red Sea sponges was investigated for the discovery of potential breast cancer migration inhibitors. Extracts of the Verongid sponges Pseudoceratina arabica and Suberea mollis were selected. Bioassay-directed fractionation of both sponges, using the wound-healing assay, resulted into the isolation of several new and known brominated alkaloids. Active fractions of the sponge Pseudoceratina arabica afforded five new alkaloids, ceratinines A–E (2–6), together with the known alkaloids moloka’iamine (1), hydroxymoloka’iamine (7) and moloka’iakitamide (8). The active fraction of the sponge Suberea mollis afforded the three known alkaloids subereamolline A (9), aerothionin (10) and homoaerothionin (11). Ceratinine B (3) possesses an unprecedented 5,7-dibrominated dihydroindole moiety with an epoxy ring on the side chain of a fully substituted aromatic moiety. Ceratinines D (5) and E (6) possess a terminal formamide moiety at the ethylamine side chain. Subereamolline A (9) potently inhibited the migration and invasion of the highly metastatic human breast cancer cells MDA-MB-231 at the nanomolar doses. Subereamolline A and related brominated alkaloids are novel scaffolds appropriate for further future use for the control of metastatic breast cancer. 相似文献
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In order to investigate DNA methylation and expression levels of myostatin (MSTN) gene in mscule and fat, 5 months of age of Bashiby sheep were selected, the promoter region and exon 1 methylation levels of MSTN gene was analyzed using bisulfite sequencing PCR (BSP). Real-time PCR was used to detect the expression level of MSTN gene in biceps femoris, femoral triceps, semitendinosus, semimembranosus, longissimus dorsi muscle and tail fat of Bashiby sheep. The results showed that the methylation probability of muscle was higher than fat, the methylation probability of biceps femoris, femoral triceps, semitendinosus, semimembranosus, longissimus dorsi muscle and tail fat were 74.2%, 74.2%, 83.2%, 83.7%, 82.1% and 25.3%, respectively. The expression levels of MSTN gene in biceps femoris, femoral triceps, semitendinosus, semimembranosus, longissimus dorsi muscle were significantly lower than tail fat in Bashiby sheep (P < 0.05), but there were no significant difference among biceps femoris, femoral triceps, semitendinosus, semimembranosus and longissimus dorsi muscle (P > 0.05).The DNA methylation was negatively correlated with the expression levels in muscle and fat of Bashiby sheep (r=-0.886, P < 0.05). 相似文献
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Summary Two German cultivars differing in blackspot susceptibility were grown in pots in 1998 and 1999 under control and water stress
conditions. Blackspot susceptibility of the tubers was defined as oxidative potential. The effect of free tyrosine, cysteine,
phenylalanine, chlorogenic and ascorbic acid on the oxidative potential was analyzed. A high concentration of ascorbic acid
led to a decrease in the oxidative potential after harvesting in 1998. No correlation was found between oxidative potential
and free tyrosine, phenylalanine or cysteine, respectively. Chlorogenic acid content increased during storage and gave a significant
correlation with discoloration, showing that it can have an effect on the oxidative potential of the tuber greater than previously
thought. Supplementing freeze-dried potato samples with pure chlorogenic acid increased the oxidative potential. Oxidative
potential seems to depend on a series of chemical compounds, and their content is affected by factors including annual variations,
cultivar, water availability and storage. 相似文献