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Krittika SRISUKSAI Kongphop PARUNYAKUL Narumon PHAONAKROP Sittiruk ROYTAKUL Wirasak FUNGFUANG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(9):1425
Diabetes mellitus (DM) is characterized by metabolic disorders and psychological deficits, including cognitive decline. Here, we investigated the effect of cordycepin on oxidative stress and protein expression in the brains of diabetic mice. Twenty-four mice were divided into four groups, one comprising untreated healthy mice (N); one comprising healthy mice treated with cordycepin (24 mg/kg body weight) (N+Cor); one comprising untreated DM mice; and one comprising DM mice treated with cordycepin (24 mg/kg body weight) (DM+Cor). After 14 days of treatment, cognitive behavior was assessed using the novel object recognition (NOR) test. The brain levels of oxidative stress markers (glutathione, catalase, and superoxide dismutase) were examined using the respective detection kits. Protein expression in brain tissues was assessed by liquid chromatography with tandem mass spectrometry (LC–MS/MS); the functions of the identified proteins were annotated by PANTHER, while major protein–protein interactions were assessed using STITCH. We found that cordycepin treatment significantly decreased body weight and food and water intake in the DM+Cor group compared with that in the DM group; however, no differences in blood glucose levels were found between the two groups. Cordycepin treatment significantly reversed cognitive decline in diabetic mice in the NOR test and ameliorated antioxidant defenses. Additionally, we identified ULK1 isoform 2, a protein associated with cognitive function via the activated AMPK and autophagic pathways, as being uniquely expressed in the DM+Cor group. Our findings provide novel insights into the cellular mechanisms underlying how cordycepin improves cognitive decline in diabetic mice. 相似文献
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Supika Vanitchung Ralf Conrad Narumon W. Harvey 《Soil Science and Plant Nutrition》2013,59(5):650-658
Nitrous oxide (N2O) emissions from the soil surface of five different forest types in Thailand were measured using the closed chamber method. Soil samples were also taken to study the N2O production pathways. The monthly average emissions (±SD, n?=?12) of N2O from dry evergreen forest (DEF), hill evergreen forest (HEF), moist evergreen forest (MEF), mixed deciduous forest (MDF) and acacia reforestation (ARF) were 13.0?±?8.2, 5.7?±?7.1, 1.2?±?12.1, 7.3?±?8.5 and 16.7?±?9.2?µg N m?2 h?1, respectively. Large seasonal variations in fluxes were observed. Emission was relatively higher during the wet season than during the dry season, indicating that soil moisture and denitrification were probably the main controlling factors. Net N2O uptake was also observed occasionally. Laboratory studies were conducted to further investigate the influence of moisture and the N2O production pathways. Production rates at 30% water holding capacity (WHC) were 3.9?±?0.2, 0.5?±?0.06 and 0.87?±?0.01?ng N2O-nitrogen (N) g-dw?1day?1 in DEF, HEF and MEF respectively. At 60% WHC, N2O production rates in DEF, HEF and MEF soils increased by factors of 68, 9 and 502, respectively. Denitrification was found to be the main N2O production pathway in these soils except in MEF. 相似文献
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