优质国审棉品种——ZHM19

概述了ZHM19的选育过程、特征特性、产量、纤维品质和抗病性等特点及主要栽培技术措施。     

Effects of natural pyrethrum and synthetic pyrethroids on the tiger mosquito,Aedes albopictus (skuse) and non-target flower-visiting insects in urban green areas of Padua,Italy

Abstract

The tiger mosquito is a key vector of several human diseases and is considered a public health concern worldwide. The implementation of strategies aimed at maximizing mosquito control without affecting non-target insect groups is of major importance. In a field trial, we tested the efficiency of a natural pyrethrum-based vs. a synthetic pyrethroid-based insecticide in reducing tiger mosquito population and how they affect the diversity of non-target flower-visiting insects in green urban areas. Only the pyrethroid insecticide was effective in reducing mosquito abundance, although its effects disappeared nine days after application. The two adulticides did not significantly affect the diversity of flower-visiting insects, probably because of their large body size and the difference in flying and foraging activity. To effectively control mosquito populations while preventing intoxication of non-target flower-visiting insects, adulticide applications should be applied early in the morning and only on bushes and trees. Results from our small-scale applications cannot be extrapolate when larger areas are treated.     

单克隆抗体ELISA检测犊牛粪便中冠状病毒抗原

用两株单克隆抗体混合后包被ＥＬＬＩＳＡ微孔板，加牛冠状病毒抗原，加经白陶土处理过的兔抗牛冠状病毒免血清，再加上辣根氧化物酶标记的羊抗兔ＩｇＧ抗体，加底物质显色间接ＥＬＩＳＡ检测牛冠状病毒抗原获得成功。用此方法检测细胞培养的牛冠状病毒上清液，其敏感度高出血凝试验（ＨＡ）８倍，从武汉市附近３个奶牛场采集犊牛腹泻粪便１０５份，用单抗ＥＬＩＳＡ检测牛冠状病毒抗原，共检出阳性３６份，并与血凝及血凝抑制试验，     

KK-42对家蚕蛹血淋巴及卵巢蛋白质合成的影响

研究了咪唑类化合物KK—42对家蚕卵巢发育以及蛋白质合成的影响,结果表明KK-42对卵巢增重有明显的抑制作用,血淋巴蛋白含量在蛹期变化幅度很小,卵巢蛋白质含量增加也很缓慢;而对照区在化蛹后,血淋巴蛋白急剧增加,第三天达到高峰,以后又迅速下降,与卵巢蛋白质增加相吻合。由此表明,咪唑类化合物KK-42不仅抑制血淋巴蛋质的合成,同时还抑制了血淋巴蛋白向卵巢的转移。     

几种抗生素对梨叶片愈伤组织和不定梢诱导的影响

研究了氨苄青霉素(Amp)、羧苄青霉素(Carb)、头孢霉素(Cef)和卡那霉素(Km)对梨叶片愈伤组织和不定梢诱导的影响。结果表明,Amp对巴梨叶片分化影响较小,当浓度达500mg/L时,其出愈率和不定梢再生率分别为96.48%和56.17%,与对照差异不显著;Cef浓度在50-300mg/L时对巴梨和身不知叶片再生均无显著影响,只有当Cef浓度高于400mg/L时,巴梨的不定梢再生率才受到显著抑制;巴梨和身不知叶片对Carb的反应不相同,低浓度(≤200mg/L)的Carb对巴梨的出愈率和不定梢再生率影响较小,却显著抑制身不知的不定梢再生率,高浓度(≥300mg/L)的Carb不仅极显著抑制巴梨和身不知的出愈率,而且完全抑制了2者的不定梢再生;Km浓度大于10mg/L时,明显抑制愈伤组织的生长,且随着浓度的增加,死亡率逐渐增大,当Km浓度达100mg/L时,巴梨和身不知叶片死亡率达100%和96.30%。     

引进扁桃的品质特性观察

从美国引进的3个扁桃品种,用山桃作砧木,在陕西渭北地区生长旺盛,苗木栽植第3年开始开花结果,5年生平均株产1.78～2.03kg。扁桃3月中下旬开花,果实前期发育较快,至5月中旬果径生长量占总量的80%～90%,后期增长缓慢。Nonpareil品种7月25日～8月5日成熟,Mission品种9月11日～9月15日成熟。成熟种仁饱满,外观品质较好。种仁含粗脂肪49.62%～52.49%,含粗蛋白20.9%～29.0%,含VB11.8～1.91mg/kg,VB24.08～4.62mg/kg。氨基酸及钙、镁等元素含量也较丰富,与我国新疆扁桃主产区产品营养含量相近似。     

Expression of cyclooxygenase-2 in IL-1β-stimulated mesangial cells is medicated by NF-κB/IκB signal pathway

AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of cyclooxygenase-2 (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE₂ concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE₂ production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway.     

水貂铜绿假单胞菌检测方法研究进展

水貂出血性肺炎是由铜绿假单胞菌引起的威胁养貂业的重要疾病之一。为了解该病相关检测方法的最新研究进展,本文从病原学和分子生物学方面,介绍了细菌分离培养、生化试验、特异性抗原及其抗体检测、PCR技术和LAMP技术等。通过比较各种方法的优缺点和实用性,为临床兽医科技工作者快速、简便、准确、实用地诊断提供参考。