全文获取类型
收费全文 | 282篇 |
免费 | 9篇 |
专业分类
林业 | 20篇 |
农学 | 14篇 |
基础科学 | 2篇 |
110篇 | |
综合类 | 13篇 |
农作物 | 27篇 |
水产渔业 | 10篇 |
畜牧兽医 | 63篇 |
园艺 | 10篇 |
植物保护 | 22篇 |
出版年
2022年 | 1篇 |
2021年 | 9篇 |
2020年 | 4篇 |
2019年 | 8篇 |
2018年 | 3篇 |
2017年 | 8篇 |
2016年 | 9篇 |
2015年 | 6篇 |
2014年 | 5篇 |
2013年 | 20篇 |
2012年 | 17篇 |
2011年 | 32篇 |
2010年 | 16篇 |
2009年 | 12篇 |
2008年 | 30篇 |
2007年 | 14篇 |
2006年 | 11篇 |
2005年 | 9篇 |
2004年 | 14篇 |
2003年 | 17篇 |
2002年 | 9篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1998年 | 4篇 |
1997年 | 7篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 5篇 |
1993年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有291条查询结果,搜索用时 0 毫秒
1.
Mark K. Asplen Gianfranco Anfora Antonio Biondi Deuk-Soo Choi Dong Chu Kent M. Daane Patricia Gibert Andrew P. Gutierrez Kim A. Hoelmer William D. Hutchison Rufus Isaacs Zhi-Lin Jiang Zsolt Kárpáti Masahito T. Kimura Marta Pascual Christopher R. Philips Christophe Plantamp Luigi Ponti Gábor Vétek Heidrun Vogt Vaughn M. Walton Yi Yu Lucia Zappalà Nicolas Desneux 《Journal of pest science》2015,88(3):469-494
2.
Stefano Pavan Zheng Zheng Mariya Borisova Petra van den Berg Concetta Lotti Claudio De Giovanni Pim Lindhout Hans de Jong Luigi Ricciardi Richard G. F. Visser Yuling Bai 《Euphytica》2008,162(1):91-98
The recessive gene ol-2 confers papilla-associated and race-non-specific resistance to tomato powdery mildew caused by Oidium neolycopersici. In order to facilitate marker assisted selection (MAS) in practical breeding programmes, we identified two simple sequence repeat (SSR) markers and one cleaved amplified polymorphic sequence (CAPS) marker which are linked to the resistance locus and co-dominantly inherited. Aiming to provide a base for ol-2 positional cloning, we used a large segregating F2 population to merge these markers with all the ol-2 linked amplified fragment length polymorphism (AFLP®) markers previously identified in an integrated genetic map. By screening a tomato bacterial artificial chromosome (BAC) library, we detected two BAC clones containing two expressed sequence tags (ESTs) homologous to the gene mlo, responsible for powdery mildew resistance in barley, as well as an ol-2-linked marker. Chromosomal mapping by Fluorescence in situ Hybridization (FISH) revealed major signals of the two BAC DNAs in the pericentromeric heterochromatin of the short arm of chromosome 4, in the same region where the ol-2 gene was previously mapped. The genetic and cytogenetic co-localisation between ol-2 and tomato mlo-homologue(s), in addition to the similarity of ol-2 and mlo resistances for both genetic and phytopathological characteristics, suggests that ol-2 is likely a mlo-homologue. Thus, a homology-based cloning approach could be more suitable than positional cloning for ol-2 isolation. 相似文献
3.
Massimiliano Cardinale Valerio Bartolino Marcos Llope Luigi Maiorano Mattias Sköld Jacob Hagberg 《Fish and Fisheries》2011,12(3):289-298
Increased knowledge on the spatial distribution of marine resources is crucial for the implementation of a true ecosystem approach to management and the conservation of marine organisms. For exploited fish species characterized by aggregation behaviour during spawning time, the identification and tracking of spawning areas is essential for a correct assessment of their productivity and population abundance. To elucidate this concept, we reconstructed the spatio‐temporal distribution of adult plaice (Pleuronectes platessa, Pleuronectidae) during spawning time along the 20th century. Historical data reveal that not only the abundance but also the former population richness was much higher than previously estimated and has declined because of protracted over‐exploitation during the last 30 years. We conclude that forecast of stock recovery to former levels of abundance neglecting spatial reorganizations might be over‐optimistic and shaded by a lost memory of the past population richness. These results reinforce the importance of managing exploited marine resources at a greater spatial resolution than has been carried out in the history of fishery management. 相似文献
4.
Gianluca Neglia Bianca Gasparrini Domenico Vecchio Marcello Rubessa Rossella Di Palo Luigi Zicarelli Giuseppe Campanile 《Tropical animal health and production》2010,42(6):1243-1247
The aim of this study was to evaluate the effect of exogenous progesterone supplementation on superovulatory response in buffaloes
that has undergone a multiple ovulation program. Fourteen Mediterranean buffaloes were divided into two groups and received
a 4-day decreasing dosage of an equal mixture of 500 IU of FSH and LH starting on day 8 of the cycle. In group A (n = 7) a progesterone-releasing intravaginal device was removed on day 8, whereas in group B (n = 7) it was left till day 10, when PGF2a {\hbox{PG}}{{\hbox{F}}_{{2}\alpha }} was administered. Eighty hours later, buffaloes were artificially inseminated and after 6 days they undergone uterine flushing.
A higher (P < 0.05) number of corpora lutea (8.3 vs. 5.7) and embryo/flushing/buffalo (2.3 vs. 1.3) were recorded in group B vs. group
A if responsive buffaloes are considered (n = 12) and the number of corpora lutea was highly correlated with the number of embryos (r = 0.65; P < 0.05). In conclusion, progesterone supplementation during the first 2 days of the superovulation treatment seems to enhance
the recovery rate in buffalo species. A high ovulation rate, associated with a high number of corpora lutea, can represent
a parameter for estimating embryo recovery. 相似文献
5.
Summary
Solanum commersonii Dun. is a diploid (2n=2x=24, 1EBN) wild species of potential value for potato breeding. It is a reproductively isolated species and cannot be crossed with Tuberosum haploids (2n=2x=24, 2EBN) or other diploid 2EBNSolanum species. In order to overcome the EBN barriers, triploid hybrids were produced between Phureja-Tuberosum haploid hybrids, which form 2n pollen grains by parallel spindles, and tetraploidS. commersonii. Microsporogenesis analysis of the triploids indicated a trend towards low values of chromosome distribution at Anaphase I; lagging chromosomes were often observed as well. Despite these abnormalities, the percentage of stainable pollen was very high, ranging from 5.0% to 74.3%. A high variation in pollen grain diameter was also evident. Parallel and tripolar orientation of spindles at Metaphase II of microsporogenesis was a common feature of all the triploids analyzed, but dyads and triads were observed at a very low frequency. Therefore, also the frequency of 2n pollen was very low; the different size of stainable pollen appears to represent the ploidy levels which are possible according to the distribution of chromosomes in Anaphase I. The results obtained also suggest thatS. commersonii could have minor genes acting at the end of meiosis in such a way that, despite the presence of parallel/tripolar spindles, dyads/triads are not formed.Contribution no. 124 from the Research Center for Vegetable Breeding. 相似文献
6.
Soil chemical and biochemical properties of a salt-marsh alluvial Spanish area after long-term reclamation 总被引:2,自引:0,他引:2
Vito Armando Laudicina Maria Dolores Hurtado Luigi Badalucco Antonio Delgado Eristanna Palazzolo Michele Panno 《Biology and Fertility of Soils》2009,45(7):691-700
Marisma, one of the largest salt-marsh alluvial areas in SW Spain, has been reclaimed since 1970 by artificial drainage and
amendment with phosphogypsum (PG) so as to reduce Na+ saturation. Within the reclaimed area, two 250- × 20-m plots were treated as follows: (1) amendment with 25 Mg/ha of PG every
2 to 3 years between 1979 and 2003 (plot PY); (2) like PY but PG treatment stopped after 1997 (plot DR). A contiguous virgin
Marisma salt-marsh plot (MV), neither drained nor amended, was the control. In MV, soil microbial biomass C, most enzyme activities
and total organic C content were much greater than in PY and DR soils, despite the salinity stress. The decrease in soil organic
matter content in PY and DR soils was likely due to cotton-cropping practices, which favoured the organic C mineralisation
and nutrients removal by crops. Microbial activity of MV soil, probably stimulated by the rhizodepositions of the natural
vegetation, did not suffer from the osmotic effect due to the raising of soil solution ionic strength. Microbial quotient
could be ranked as MV > PY > DR, whereas the metabolic quotient had an opposite trend. Thus, both quotients suggested that
the interruption of PG amendment was not favouring microbial activity. Principal component analysis clearly identified the
chemical and biochemical soil properties mostly affected by the reclamation and also indicated the longer PG amendment in
PY plot. Stepwise discriminant analysis identified two physiologically different types of soil microflora, one less active
present in the MV virgin soil and another more active present in the reclaimed PY and DR soils. 相似文献
7.
利用智能LCR测量仪和平板电极系统,用无损检测方法测定了桃子在储藏过程中电特性的变化。结果表明:在12Hz~100kHz的频段内,桃子的最佳测试频段为15kHz以下,此时桃子随着储藏时间的增加,等效阻抗增大而相对介电常数和介质损耗因数减小;当桃子开始腐烂时,电特性数值出现了一个大的反复 相似文献
8.
Thibaut Olivier Vaidevutis Šveikauskas Elisabeth Demonty Kris De Jonghe Pascal Gentit Mojca Viršček-Marn Sabine Grausgruber-Gröger Sébastien Morio Francesco Faggioli Michèle Visage Frédéric Fauche Maria Gusina Marta Luigi Helena Lasner Irena Mavrič-Pleško 《European journal of plant pathology / European Foundation for Plant Pathology》2016,144(3):645-654
9.
Pier Luigi Sacco Guido Ferilli Giorgio Tavano Blessi Massimiliano Nuccio 《Growth and change》2013,44(4):555-570
Building on the early works of Alfred Marshall, analyses of local economies have emphasized the spatial function of clusters and industrial districts in terms of external economies of localization and agglomeration. Recent literature has emphasized the importance of culture and the complementarities between culture and local tangible and intangible assets. This paper aims to provide an analytical foundation for these processes with a view to developing tools for policy design, analysis and evaluation. By “system‐wide cultural districts,” we refer to a new approach to local development where cultural production and participation present significant strategic complementarities with other production chains. In this view, culture drives the accumulation of intangible assets such as human, social, and cultural/symbolic capital, thereby fostering economic and social growth and environmental sustainability. 相似文献
10.
Multi#ex—CAPS技术及其在番茄遗传鉴定中的应用 总被引:6,自引:1,他引:6
:应用Multiplex—PCR和CAPS(Cleaved amplified polymorphic sequence)原理研究建立了Mutiplex—CAPS技术。该技术包括:(1)在PCR扩增反应体系加入2对、3对或多对核苷酸引物,经30~40个扩增循环后得到每对引物的特异扩增片段;(2)应用同一种限制性内切酶消化这些不同引物经扩增后获得的特异片段;(3)琼脂糖凝胶电泳溴化乙锭染色检测酶切片段长度的多态性。利用该技术对番茄(Lycopersicon es—cdentum)TA517及其近等基因系的分析表明,Multiplex—CAPS能同时检测2个、3个或多个CAPS标记的多态性,其效果等同于每个CAPS标记的多态性的叠加,重复性好,分析效率高,降低成本数倍。 相似文献