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L C Leigh 《Research in veterinary science》1975,18(3):282-287
Young Friesian steers were fed for nine months on copper supplemented or depleted diets. Immediately after they had been killed, tissues were taken from the myocardium for examination by electron microscopy. Copper deficiency was associated with extensive changes in myofibrillar and mitochondrial morphology and distribution. It is suggested that the ultra-structural changes in the myocardium were specifically attributable to copper deficiency, the most likely underlying biochemical defect being lost of cytochrome oxidase activity. 相似文献
3.
Claire N. Lieske James H. Clark Howard G. Meyer Leigh Boldt Martin D. Green John R. Lowe Walter E. Sultan Peter Blumbergs Matthew A. Priest 《Pesticide biochemistry and physiology》1984,22(3):285-294
The inhibition of eel acetylcholinesterase by the 4-nitrophenyl esters of 2-furyl(methyl)-, methyl(2-thienyl)-, di-2-furyl-, and di-2-thienylphosphinic acid (I, II, III, and IV, respectively) was investigated at pH 6.90 in 0.067 M phosphate buffer (25.0°C) using stopped-flow instrumentation and automated data processing. Our evaluation of the dissociation constant, Kd, the unimolecular bonding rate constant, k2, and the bimolecular reaction constant, ki, are the first reported values for these constants for alkyl/heteroaryl and diheteroaryl esters of phosphinic acids. The largest ki value (19,330 M?1 sec?1) was observed for the reaction of I with the enzyme. The order for the remaining three is II > IV > III. There is no direct relationship between the hydrolysis rates of the esters and their anticholinesterase activities on eel acetylcholinesterase. Likewise, there is no direct relationship between their anticholinesterase activities and the LD50 values in rats. 相似文献
4.
Multilocus-sequence typing (MLST) was used to analyse Streptococcus uberis isolates from a single herd associated with long duration (50-260 days) and rapidly cleared (less than 1 month) bovine intramammary infections to determine whether the bacterial type had any impact on the duration of infection. Most chronic infections (24 of 33) were due to continuous infection of the mammary quarter with the same sequence type, and infections were found to persist for many months. The remaining quarters were re-infected with a different sequence type within a single lactation. No particular sequence type or clonal complex (lineage) was associated with persisting infections, indicating that the outcome of intramammary infections with S. uberis is more likely to be dependent on host factors than on inter-strain differences. Analysis of these strains alongside others obtained from the same herd at a later date revealed the shift in the predominant genotypes with time. 相似文献
5.
Leigh Ann Clayton Tracey K Ritzman 《Veterinary Clinics of North America: Exotic Animal Practice》2006,9(3):511-518
A 6-year-old cockatiel was diagnosed with egg binding based on clinical signs, physical examination findings, radiographs, and lack of oviposition. Medical management failed to induce oviposition, and surgical salpingotomy was used to remove the egg from the oviduct. Sterile egg yolk peritonitis was diagnosed based on histology. Postoperative management with leuprolide acetate and husbandary changes successfully prevented egg production for at least 2.5 months after the event. 相似文献
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Aznar I McGrath G Murphy D Corner LA Gormley E Frankena K More SJ Martin W O'Keeffe J De Jong MC 《Veterinary microbiology》2011,151(1-2):104-111
The principal wildlife reservoir of Mycobacterium bovis in Ireland is the European badger. Studies in the Republic of Ireland (RoI) have shown that badgers culled in association with cattle herd tuberculosis breakdowns (focal culling) have a higher prevalence of infection than the badger population at large. This observation is one rationale for the medium term national strategy of focal badger culling. A vaccination strategy for the control of bovine tuberculosis (bTB) in badgers is a preferred long-term option. The Bacillus Calmette-Guérin (BCG) vaccine has been shown to decrease disease severity in captive badgers under controlled conditions. As the vaccine has been tested in a controlled environment with precise information on infection pressure, it cannot be assumed a priori that the effects of vaccination are similar in the wild, where other environmental and/or ecological factors prevail. For this reason we have designed a vaccine field trial to assess the impact of vaccination on the incidence of TB infection in a wild badger population. The selected study area for the vaccine trial (approximately 755 square kilometers) is divided into three zones each of which has similar characteristics in terms of size, number of main badger setts, cattle herds, cattle and land classification type. Three vaccination levels (100%, 50% and 0%) will be allocated to the three zones in a way that a gradient of vaccination coverage North to South is achieved. The middle zone (zone B) will be vaccinated at a 50% coverage but zone A and C will be randomly allocated with 100% or 0% vaccination coverage. Vaccination within zone B will be done randomly at individual badger level. The objective of this paper is to describe the design of a field tuberculosis vaccination trial for badgers, the epidemiological methods that were used to design the trial and the subsequent data analysis. The analysis will enable us to quantify the magnitude of the observed vaccination effect on M. bovis transmission in badgers under field conditions and to improve our knowledge of the biological effects of vaccination on susceptibility and infectiousness. 相似文献
8.
Joanna Lodzinska Hannah Leigh Magdalena Parys Tiziana Liuti 《Veterinary radiology & ultrasound》2019,60(6):745-752
Peripheral vein phlebitis (inflammation) is a relatively frequent complication in dogs, however, published information on the ultrasonographic characteristics is currently lacking. This prospective, observational study describes the ultrasound (US) characteristics of normal canine cephalic veins, and veins with clinical phlebitis. Correlations among US findings and between US findings versus time that the intravenous catheter was in place were investigated. Safety of the US procedure was evaluated. Fifty patients were prospectively recruited for the study and 18 met the final inclusion criteria. Each patient underwent daily US examinations and was assessed for multiple criteria (vascular wall appearance, compressibility, spontaneity of flow, color fill, and presence/absence of filling defects, flow contour, direction, non‐pulsatility). Characteristics of normal canine cephalic veins were as follows: smooth and thin wall, complete compressibility, no flow disturbances, no filling defects, smooth flow contours, and unidirectional, non‐pulsatile flow with no turbulence. Characteristics of cephalic veins with clinical phlebitis were as follows: wall thickening (83%), decreased compressibility (55%), filling defects consistent with intraluminal thrombus (55%), vessel wall hyperechogenicity (44%), and abnormal color Doppler flow (39%). Significant correlations were found between Doppler filling defects and compressibility, Doppler filling defects and presumed thrombosis, and compressibility and presumed thrombosis (P = .001, P = .001, P = .000, respectively). No correlation was found between the US findings and time the intravenous catheter was in place. Findings indicated that duplex and compressibility US are feasible and safe methods for characterizing and monitoring cephalic veins in dogs with clinical phlebitis. 相似文献
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- 1 Fluvial maintenance is frequently undertaken to preserve the flood capacity, visual amenity, conservation value and geomorphic stability of managed river channels. Maintenance tasks include the management of both riparian and in-channel vegetation and maintenance dredging.
- 2 Riparian vegetation is traditionally managed by physical methods such as cutting of grasses or removal of trees. Less environmentally severe alternative practices include grazing or shading for grasses, and practices such as pollarding or coppicing for trees.
- 3 While a range of alternative maintenance practices, with varying environmental impact, are usually available for river managers to select, the potential for improving maintenance practices varies according to the particular task considered and the constraints imposed by the need to reach and maintain the target standard of service in terms of flood defence and land drainage.
- 4 This review shows that economic and environmental impacts associated with fluvial maintenance operations may be reduced at three scales. First, at the smallest scale, it is shown that there is often potential for improving the local operational efficiency of individual fluvial maintenance tasks. Second, it may be possible to reduce the intensity of maintenance in channel reaches which are presently over-serviced. Third, at the largest scale, it is shown that efficient maintenance is best achieved within the framework of Integrated Basin Management, and by giving appropriate consideration to future maintenance requirements at the design stage of new projects to reduce the overall need for fluvial maintenance. Examples of the way in which these policies may be implemented to reduce environmental impact without compromising engineering objectives are illustrated through case studies from the UK and the USA.