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1.
Daisuke Shiode Yasuzumi Fujimori Fuxiang Hu Susumu Shimizu Teisuke Miura 《Fisheries Science》2001,67(1):110-117
SUMMARY: Flume-tank experiments were performed to examine the depth response of a new type of depth-controlled gillnet. Variations of net depth were investigated as the warp was paid out and wound up for different changes of warp length, main sinker weights, and winch speeds. In most experiments, when the warp finished paying out, the net continued to descend and then ascended slightly to an equilibrium depth (overshoot phenomenon). The overshoot distance was nearly constant when the warp was wound up and increased linearly with increasing winch speed when the warp was paid out. An increase in winch speed reduced net settling time, which converged on a constant value for both paying out and winding up. 相似文献
2.
K Adachi A Yoshimoto T Hasegawa T Shimizu Y Goto S Makimura 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(6):1081-1084
Due to the potential for anti-erythrocyte membrane antibodies as possible enhancers of erythrocyte destruction, the presence of serum anti-erythrocyte membrane antibodies in 31 dogs with Babesia gibsoni infection admitted to a veterinary hospital was investigated by an enzyme linked immunosorbent assay (ELISA) and immunoblotting analyses. This infection resulted in an increase of anti-erythrocyte membrane antibodies in 84% (IgG) and 74% (IgM) of 31 infected dogs, respectively. This was confirmed by the similarity in the protein profiles of the erythrocyte membrane antigens immunoblotted with rabbit antiserum to dog erythrocyte membrane antigens and infected dog serum. These results suggest the production of anti-erythrocyte membrane antibodies was induced by B. gibsoni infection. 相似文献
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4.
Yuki Ichinose Rena Shimizu Yoko Ikeda Fumiko Taguchi Mizuri Marutani Takafumi Mukaihara Yoshishige Inagaki Kazuhiro Toyoda Tomonori Shiraishi 《Journal of General Plant Pathology》2003,69(4):244-249
The flagellins purified from Pseudomonas syringae pv. tabaci induce a hypersensitive reaction in nonhost tomato cells. To investigate the role of flagella and flagellin in the compatible interaction, we generated two types of flagella-defective mutant. The fliC mutant lost the fliC gene that encodes flagellin protein, whereas the fliD mutant lost the fliD gene that encodes HAP2-capping protein. The two mutants had markedly reduced ability to cause disease symptoms in tobacco leaves. Furthermore, propagation of the mutants in tobacco leaves was less than that in wild-type pv. tabaci. Compared to the inoculation with wild-type pv. tabaci, inoculation with the two mutants did not markedly induce the expression of typical defense response-related genes such as PAL and hsr203J. Complementation of each fliC and fliD gene to the corresponding deficient mutant restored motility and virulence. These results indicate that flagella of P. syringae pv. tabaci are indispensable organelles for complete virulence on host tobacco plants. 相似文献
5.
Thyroid hormone augments GLUT4 expression and insulin-sensitive glucose transport system in differentiating rat brown adipocytes in culture 总被引:1,自引:0,他引:1
Shimizu Y Shimazu T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(8):677-681
The effects of triiodothyronine (T3) on differentiation-dependent expression of GLUT and responses of glucose transport to insulin and norepinephrine (NE) were investigated. Precursor cells of brown adipocytes isolated from the interscapular brown adipose tissue of newborn rats were cultured in the absence or presence of various concentrations of T3. Western bolt analysis revealed that treatment with T3 resulted in an increased expression of GLUT4, in a dose-dependent manner, whereas GLUT1 contents were unchanged. In parallel with the increase in GLUT4 expression, T3 improved insulin sensitivity for glucose transport, being accompanied by an increase in maximal transport rate and a reduction of ED(50). In contrast, T3-treatment of the brown adipocytes during the differentiation process had little effect on NE-regulatable glucose transport system. These results suggest that T3 plays a predominant role in the development of insulin-sensitive glucose transport during differentiation of brown adipocytes. 相似文献
6.
Hideo Ishii Junko Tanoue Michiyo Oshima Wen-Hsin Chung Kumiko Nishimura Junichiro Yamaguchi Fumihiro Nemoto Kazuhiro So Toshitaka Iwama Hideaki Yoshimatsu Motoshige Shimizu Toru Kozawa 《Journal of General Plant Pathology》2008,74(6):409-416
Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins.
Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice
blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine
at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single
nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes
coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing
Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report
is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences. 相似文献
7.
Koiwai M Hamaoka T Haritani M Shimizu S Zeniya Y Eto M Yokoyama R Tsutsui T Kimura K Yamane I 《Veterinary parasitology》2006,135(2):175-179
Serum samples from 2420 clinically healthy dairy cattle, randomly selected from stored sera in 18 districts of Japan, were tested for the presence of Neospora caninum antibodies using an indirect fluorescent antibody test (titer > or =1:200). Nationwide seroprevalence is estimated at 5.7% (139/2420). Seropositive cattle were detected in all surveyed districts despite the evidence of confirmed case reports of bovine neosporosis, showing that N. caninum is widely distributed throughout Japan. Age-specific seroprevalence did not increase with cattle age, suggesting that Neospora infection is likely to be transmitted vertically rather than horizontally in Japan. Considering that N. caninum seropositive cows are thought to be more likely to abort, substantial fetal losses may be induced by N. caninum infection in Japan. Devising strategies are needed to reduce the economic impact on the Japanese dairy industry. This is the first study to investigate the nationwide seroprevalence of N. caninum in cattle in Asia. 相似文献
8.
Harada Kousuke Kato Yuria Takahashi Jun Imamura Hiyoko Nakamura Natsume Nishina Atsuyoshi Phay Nyunt Tadaishi Miki Shimizu Makoto Kobayashi-Hattori Kazuo 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(1):103-109
Plant Foods for Human Nutrition - Adipose tissue is an endocrine organ and its endocrine function is closely associated with type 2 diabetes mellitus. Valeriana officinalis (Valerian) exerts some... 相似文献
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10.
A Shimizu J Kawano T Fujinami J Ozaki S Kimura K Sugihara 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(5):963-968
Staphylococci were found in the tonsils of 121 (75.2%) of 161 cattle. There were 15 different species, 10 belonging to novobiocin-sensitive species. The most predominant species was S. simulans (79.3% of the 121 carriers), followed by S. aureus (20.7%), S. chromogenes (10.7%) and S. epidermidis (8.3%). The other 11 species were present in 0.8 to 5.8%. Twenty-six unidentifiable isolates were isolated from 26 (21.5%) carriers. Sixty-two (51.2%) of the 121 carriers yielded two to five Staphylococcus species together while only one species could be found in each of the other 59 (48.8%). Combinations of S. simulans and other species were most frequently encountered in 50 (41.3%) of the 121 carriers. Twenty-four (96.0%) out of 25 S. aureus isolates, 3 (42.9%) of 7 S. hyicus isolates and 45 (25.4%) of 177 coagulase-negative staphylococci (13 species and unidentifiable isolates) isolates were phage typable. Most of S. aureus isolates were lysed by bovine phages 119 (n = 16) or 116 (n = 5). Thirty-three (25.4%) of 45 coagulase-negative staphylococci typable isolates with Pulverer's phage set showed the phage pattern ph5/ph9/ph10/ph12/ph13/U4/U14/U16/++ +U20/U46. The tonsils of cattle thus appear to be a suitable environment for Staphylococcus species, particularly novobiocin-sensitive species. 相似文献