Fecundity changes in Macrobrachium rosenbergii (de Man) during egg incubation

Abstract. For a study of the fecundity of Macrobrachium rosenbergii , a total of 117 berried females were sampled from ponds. Fecundity was found to vary from 1216 to 89747 eggs for prawns measuring 9.0cm to 15.8cm total length and weighing 6.22 to 45.80g. The relationship between fecundity (EN) and total length (TL) was found to be EN = 0.001876 TL^6.3617.
The average egg number/g total body weight for prawns having orange, yellow and grey eggs was 1132.7 ± 484.1,766.4 ± 524.3 and 745.5 ± 487.2, respectively, a decrease of 32.3% when orange eggs became yellow and 34.3% when they turned grey. This decrease was probably due to unfertilized eggs dropping off and some eggs being eaten by the brooders during the incubation period.
Egg numbers/g egg weight were found to be 11360.1 ± 1642.1,10715.7 ± 2725.3 and 8634.0 ± 1892.3 for orange, yellow and grey eggs, respectively. When compared to orange eggs, yellow eggs were slightly heavier (6%), but grey eggs were significantly heavier by 31.6%. The increase in the weight of the grey eggs can be explained by the elongation of fully formed organs and appendages by about 17 days post fertilization.     

Impacts of wetting-drying cycles on short-term carbon and nitrogen dynamics in Amynthas earthworm casts

Effects of earthworm casts on soil nutrient dynamics and their responses to changing moisture availability in subtropical ecosystems remain poorly understood. This study aimed to examine short-term carbon(C) and nitrogen(N) dynamics and their interactions with wetting-drying cycles in three different structural forms(i.e., granular, globular, and heap-like) of Amynthas earthworm casts. The rates of C and N mineralization in the earthworm casts were examined under two different wetting-drying cycles(i.e., 2-d and 4-d wetting intervals) using a rainfall simulation experiment. After three simulated rainfall events, subsamples of the earthworm casts were further incubated for 4 d for the determination of CO_2 and N_2O fluxes. The results of this study indicated that the impacts of wetting-drying cycles on the short-term C and N dynamics were highly variable among the three cast forms, but wetting-drying cycles significantly reduced the cumulative CO_2 and N_2O fluxes by 62%–83% and 57%–85%, respectively, when compared to the control without being subjected to any rainfall events. The C mineralization rates in different cast forms were affected by the amount of organic substrates and N content in casts, which were associated with the food preference and selection of earthworms. Meanwhile, the cumulative N_2O fluxes did not differ among the three cast forms. Repeated wetting and drying of casts not only enhanced aggregate stability by promoting bonds between the cast particles, but also inhibited microbial survival and growth during the prolonged drying period, which together hindered decomposition and denitrification. Our findings demonstrated that the interactions between the structural forms, aggregate dynamics, and C and N cycling in the earthworm casts were highly complex.     

Esophageal Replacement in the Dog by Microvascular Colon Transfer

An orthotopic colon graft based on the middle colic artery and vein was implanted with microvascular technique and a stapling instrument in five dogs. The grafts were successful in four dogs. A similar colon autograft was used to replace the entire thoracic esophagus in five dogs. The recipient vessels were the left carotid artery and left external jugular vein. Four of the grafts failed because of kinking and thrombosis of the arterial supply (2 dogs) or the venous outflow (2 dogs). One graft, which had a viable vascular supply, developed a severe leak at the colon-to-stomach anastomosis, and the dog was euthanatized on day 3. The recipient vascular pedicle was modified and used successfully to replace a portion of the cervical esophagus in three dogs. The grafts survived, the dogs could swallow liquids and semisolid food well, and, at necropsy after 4 weeks, the anastomotic sites were well healed. The graft sites contained essentially normal colon mucosa.     

Some Effects of the Plantation of Conifers on a Freely Drained Lowland Soil, Forest of Dean, U.K.

Acid brown soils under 50-year-old conifer plantations are comparedwith those at a control site under the previous vegetation ofmixed deciduous woodland. The soils under the conifers showsignificant signs of podzolization and associated with this,evidence of structural breakdown. The implications of thesechanges are briefly discussed.     

The Effect of Starch Amendment on Nitrogen Mineralisation from the Forest Floor Beneath a Range of Conifers

Samples of LFH collected beneath the canopies of 16 coniferousspecies growing at the same site were incubated in the laboratorywith or without a starch amendment. Exchangeable nitrogen presentranged from 10.5–256 and 11.4–512 µgg^–1organic matter for freshly collected and unamended incubationsrespectively. When starch was added material from 11 speciesshowed significantly greater accumulation of mineral nitrogenwith increases ranging from ca 20–1370 per cent. Nitrificationwas negligible except in Thuja plicata where nitrate constituted68 or 83 per cent of the mineral nitrogen present after incubationwithout or with starch respectively. Starch addition increasedrates of net nitrogen mineralisation in LFH material from Piceasitchensis over a range of growth rates. Rates were greatestin material from the most productive stands whether or not starchwas added. The results are discussed with reference to the factorscontrolling litter decomposition and nitrogen mineralisationin the forest floor.     

Influences of mean advection and simple behavior on the distribution of cod and haddock early life stages on Georges Bank

Results of a modeling study designed to explore the influences of physical advection and certain biological mechanisms on the distribution of cod (Gadus morhua) and haddock (Melarwgrammus aeglefinus) early life stages on Georges Bank are described. Using a late-winter/early-spring 3-D circulation field driven by the M₂ tidal current, mean wind stress and Scotian Shelf inflow, we examine the distribution of cod and haddock larvae spawned on the Northeast Peak of the Bank. The sensitivity to a March-April baroclinic field is also explored. Results indicate that larvae remaining in the surface Ekman layer are generally advected off-bank. However, downwelling associated with Ekman layer convergence near the shelf break provides a mechanism for larvae to exit from the off-bank surface drift. Larvae below the surface layer are transported south-westward along the southern flank of Georges Bank and are retained on the Bank if their position immediately upstream of the Great South Channel is shoalward of (roughly) the 70 m isobath. Within the Great South Channel region and between the 50 and 70 m isobaths, retention can depend on the phase of the tide. Spawning shoalward of the 50 m isobath on the Northeast Peak greatly increases the chances of retention. These results apply to passive larvae and to those with specified vertical distributions and migration based on observations. Directional on-bank swimming at rates of 0.5 to 1 body length per second would substantially enhance shoalward displacement, resulting in larval distributions during the first 2 months that are consistent with field observations.     

Sampling error resulting from depth-dependent plankton distributions and current differentials in a two-layered system

We evaluate a source of error observed in standard ichthyoplankton bongo tows in which volumes of water filtered did not result in increased catches of red-fish. Our analyses demonstrate that, under conditions where redfish larvae are distributed in surface waters in a two-layered system, current differentials between surface and subsurface layers resulted in biased, standardized estimates of larval abundances as a result of filtering varying amounts of subsurface waters. Analysis of standard survey results on Flemish Cap demonstrated that this source of bias was limited to the May-June period, after waters became stratified and before redfish passed from the larvae to pelagic juvenile stage. Correcting for this source of sampling bias wIII result in more accurate absolute estimates of ichthyoplankton abundance, and we estimate that variation (CV) for replicate samples may be reduced by 10%.     

RADIOGRAPHIC EVALUATION OF FELINE APPENDICULAR DEGENERATIVE JOINT DISEASE VS. MACROSCOPIC APPEARANCE OF ARTICULAR CARTILAGE

Degenerative joint disease (DJD) is common in domesticated cats. Our purpose was to describe how radiographic findings thought to indicate feline DJD relate to macroscopic cartilage degeneration in appendicular joints. Thirty adult cats euthanized for reasons unrelated to this study were evaluated. Orthogonal digital radiographs of the elbow, tarsus, stifle, and coxofemoral joints were evaluated for the presence of DJD. The same joints were dissected for visual inspection of changes indicative of DJD and macroscopic cartilage damage was graded using a Total Cartilage Damage Score. When considering all joints, there was statistically significant fair correlation between cartilage damage and the presence of osteophytes and joint‐associated mineralizations, and the subjective radiographic DJD score. Most correlations were statistically significant when looking at the different joints individually, but only the correlation between the presence of osteophytes and the subjective radiographic DJD score with the presence of cartilage damage in the elbow and coxofemoral joints had a value above 0.4 (moderate correlation). The joints most likely to have cartilage damage without radiographic evidence of DJD are the stifle (71% of radiographically normal joints) followed by the coxofemoral joint (57%), elbow (57%), and tarsal joint (46%). Our data support radiographic findings not relating well to cartilage degeneration, and that other modalities should be evaluated to aid in making a diagnosis of feline DJD.     

Magnetic resonance imaging in two dogs with central nervous system disease

Accurate localization of the lesions in two dogs with progressive neurological disease was demonstrated with magnetic resonance imaging (MRI). The first dog had unilateral cerebellar signs with associated paradoxical vestibular symptoms. The CSF tap and clinical localization suggested a right-sided cerebellar tumour and this was confirmed with MRI scanning. The second dog had predominantly asymmetrical fore-brain signs with circling, personality changes, seizures and contralateral proprioceptive deficits. CSF analysis suggested an inflammatory or neoplastic condition. MRI showed a diffuse oedematous lesion of the left cerebral hemisphere which corresponded exactly with the lesions seen at necropsy. The advantages of MRI over CT scans are discussed.     

A progressive axonopathy of Boxer dogs affecting the central and peripheral nervous systems

Five Boxer dogs with a new neurological disease are described. There is a progressive ataxia and weakness, initially in the hind-legs but later involving forelegs. The onset of signs is usually around 6 months of age although an older dog was affected. Proprioceptive function, muscle tone and tendon reflexes are diminished or absent while pedal reflexes and pain sensation are preserved. Muscle atrophy is minimal. The peripheral nerves are depleted in myelinated fibres. Nerve roots and, to a lesser extent, peripheral nerves show demyelination/remyelination changes and in the cervical ventral roots in particular, regenerative clusters are present. Forelimb muscles show evidence of re-innervation. In the CNS numerous swollen axons are present. The dorsolateral and ventral columns of the spinal cord are affected throughout their length. In the brain a number of nuclei and tracts, particularly in the lower brain stem, are affected and the superior olives are severely involved. The cerebral cortex and subcortical white matter appear normal. Various portions of the auditory and optic pathways are affected pathologically as are a number of cranial nerves. The axonal swellings contain a number of organelles including smooth endoplasmic reticulum, disordered neurofilaments, vesicles and mitochondria. The disease may be specific to Boxer dogs and may be inherited.