Experiments on Curative Low and Ultra-low Volume Spraying Against Dacus oleae Gmel.

Applications of 1, 2, 4, 6 and 12 lit. of spray per tree, each volume containing 6 g. of dimethoate a.i., did not show significant differences between the treatments, but olive fly pupae and third instar larvae were found to be more resistant than first and second instar larvae. Also, no differences became apparent between treatments with Dimecron 50, Carbicron 50 and Nuvacron 50 at rates of 7, 10 and 14 c.c. per tree respectively; these experiments will be continued.     

Absence of three known benzimidazole resistance mutations in Trichostrongylus tenuis, a nematode parasite of avian hosts

Benzimidazole (BZ) resistance is widespread in nematode parasites of livestock, but very little is known about the levels of BZ resistance in parasites with avian hosts. We investigated BZ resistance in Trichostrongylus tenuis, a nematode parasite of red grouse, Lagopus lagopus scotica. BZ anthelmintics had been in use in this system for up to 15 years, yet existing phenotypic evidence for resistance was inconclusive. We screened 1530 individuals from 14 populations at the principal β-tubulin locus for BZ resistance (isotype 1, residue 200) and 940 of these at two further resistance sites (isotype 1, residue 167; isotype 2, residue 200). No BZ resistant genotypes were found. Alternative mechanisms may be responsible for BZ resistance in this system, or the method and timing of treatments may reduce selection pressure for BZ resistance by creating substantial refugia for susceptible genotypes.