The Effect of Diet on the Biochemical Composition of Juvenile Artemia: Potential Formulations for Rock Lobster Aquaculture

The lipid class and fatty acid (FA) composition of juvenile Artemia fed continuously on four diets—the microalga Tetraselmis suecica , a mix of oat bran-wheat germ-lecithin (OWL), OwL-eicosapentaenoic acid (EPA), and OWL-EPA-arachidonic acid (AA)—were examined over a 9-d experiment in an attempt to approximate the FA profile of phyllosoma larvae of wild southern rock lobster Jasus edwardrii . The main difference in lipid class composition of Artemia fed the four diets was the relative level of polar lipid (PL) and triacylglycerol (TAG). By day 9, the algal-fed Artemia were highest in PL (95% of total lipid) and lowest in TAG (2%), whereas the remaining diets resulted in Artemia with 16–30% PL and 41–82% TAG. After 2 d, the relative FA composition of all Artemia treatments closely reflected those of the diets, with no marked change after further feeding (to day 9). In terms of the content of essential polyunsaturated fatty acids (PUFA), by day 5 Artemia fed: 1) with the algal diet contained 7 mg/g FA dry mass (0.3% DHA, 6.3% EPA, 3.4% AA of total FA); 2) with the OWL diet contained 3 mg/g (0.3% DHA, 0.9% EPA, 0.7% AA); 3) with the OWL-EPA diet contained 55 mg/g (6.2% DHA, 11.6% EPA, 1.1% AA); and 4) with the OWL-EPA-AA contained 83 mg/g (3.8% DHA, 7.5% EPA, 17.4% AA). The PUFA profiles of Artemia using the OWL-oil diets were similar to wild rock lobster phyllmmata, although levels of doco-sahexaenoic acid (DHA) were lower (10% DHA) than in J. edwardsii larvae. On the basis of PUFA composition data alone, the results suggest the suitability of the OWL-oil mixed diets for consideration for feeding to Artemia used in the culture of southern rock lobster larvae, particularly if the level of DHA can be further enhanced.     

Effects of Stocking Sac-Fry and Hatchery-Fed Fry on Production of Fingerling Channel Catfish Ictalurus punctatus

Abstract.— Channel catfish Ictalurus punctatus fry are typically held under hatchery conditions for 7 to 14 d after hatching to allow feeding and growth before they are stocked into nursery ponds to produce fingerling catfish. In an attempt to reduce hatchery operating costs, several catfish fingerling producers in Louisiana presently stock fry within 2 d after hatching before yolk absorption is complete. Fry at this stage of development are commonly referred to as "sac-fry." Although research has shown that fry can be stocked at the onset of yolk absorption with no detrimental effects on subsequent fingerling production, stocking sac-fry has been reported to result in reduced fingerling survival. To further investigate this topic, production trials were conducted in experimental outdoor pools over the course of two growing seasons to evaluate the effect of stocking fry of three different ages (2-, 7-, and 14-d post-hatch, DPH) on survival, growth (weight and length), condition factor (K), yield, feed consumption, and feed conversion ratio (FCR) of fingerling catfish. Results from both trials indicated that the age at which fry were stocked had no effect on production characteristics with the exception of growth. Specifically, fingerlings reared from fry stocked at 2 and 7 DPH were significantly larger than fingerlings reared from fry stocked at an age of 14 DPH. These findings suggest that the practice of stocking sac-fry may be a suitable alternative to the traditional procedure of holding and feeding fry under hatchery conditions prior to stocking. However, in order to fully evaluate the effects of early-age stocking of catfish fry on fingerling production, additional studies must be conducted under pond conditions. Furthermore, these studies must be coupled with a rigorous economic analysis before the practice of stocking sac-fry can be recommended to the catfish industry.     

Micromorphology of soils in soliflucted materials,Okstindan, Northern Norway

The micromorphology of two soil profiles developed on turf-banked solifluction lobes and a third profile formed on a larger turf-banked solifluction terrace is described. Four elements common to the three profiles are identified.In the horizontal plane the apparent long axes of detrital grains show preferred orientation parallel to the azimuth of the line of greatest slope, and in slides cut in the vertical plane aligned with the slope direction a somewhat stronger preferred orientation (or apparent dip of the grains) is observed parallel, or sub-parallel to the ground surface gradient. Secondly, coatings of matrix material are observed on the upper surfaces of detrital grains at depths greater than 50 cm. Above this depth similar coatings also appear on the sides and lower surfaces of detrital grains. Roughly horizontal flattened patches of matrix enrichment are also observed in the three profiles. These become more frequent with depth, producing a banded pattern of matrix concentrations separated by detrital grains. Finally, large rounded pores, or vesicles, are observed in the upper 25 cm of the three profiles.These micromorphological elements are described, and suggestions are made as to their origins.     

THE EFFECT OF NITROGEN APPLICATION AND AUTUMN MANAGEMENT ON AUTUMN GROWTH, WINTER 'BURN' AND SPRING GROWTH OF LOLIUM PERENNE L. AT ABERYSTWYTH, EDINBURGH AND CAMBRIDGE.

A trial was carried out to find management practices which would permit the best discrimination for winter performance of erennial ryegrass cultivars. Due to the mild winter experienced only a few of the plants were killed outright. The cultivars were therefore assessed for percentage of green herbage and for spring growth. There were four sites: an upland and lowland site at Aberystwyth and one site each at Edinburgh and Cambridge. At all sites two N rates (totals of 125 or 550 kg/ha in the first year after sowing) and 5 autumn cutting treatments were used to give differences in the amounts of herbage remaining at the onset of winter. The last dates of defoliation in the autumn were: C₁ end of August; C₂ end of September; C₃ end of October; C₄ mid-November; C₅ was cut on all these dates. Four cultivars (Grasslands Ruanui, S321, Premo and Argo) which differed in their autumn growth potential, frost susceptibility and degree of winter dormancy were grown. The autumn yield of S321 was, in general, higher than that of the other cultivars but there were interactions with N, management and location. The data on percentage of herbage remaining green in February illustrated differences due to the siting of the trial. In the conditions prevailing the two Aberystwyth sites facilitated greater discrimination between cultivars than those at Edinburgh and Cambridge. In three of the four sites the management which produced most winter ‘burn’(including both that due to natural senescence and that due to winter damage) involved accumulation of herbage in the autumn but there were considerable sites × managements interactions. For example at Cambridge management produced no significant effect whereas at the Aberystwyth lowland site frequent defoliation in the autumn had more effect than allowing herbage to remain uncut after the end of August. High N significantly decreased the percentage of green herbage only at the two Aberystwyth sites. Over five-fold differences in spring yield were obtained, the heaviest yields being recorded at Edinburgh and Cambridge. However, there were large interactions between environments and cultivars; for example the higher N rate reduced spring yield at Aberystwyth but increased spring yield at Edinburgh and Cambridge. Premo and S321 had similar yields in spring at the Aberystwyth lowland and Cambridge sites but Premo was higher yielding than S321 at the Aberystwyth upland site and at Edinburgh. The large environmental effects and their interactions with cultivars illustrate the difficulties of cultivar assessment and the dangers inherent in national recommendations for grass cultivars.     

Cooperating with industry to teach food-animal medicine

Cooperative programs with agribusiness offer opportunities for colleges of veterinary medicine to expand their instructional programs in agricultural practice. Agribusinesses associated with livestock agriculture recognize the importance of veterinary medicine in maintaining a vibrant and successful industry. Stewardship of corporate support involves close communication with advocates within the companies, providing them with documentation of the potential effects of corporate investments. This article describes the creation of the Michigan State University (MSU) Training Center for Dairy Professionals, a key aspect of which was the identification of innovative and productive areas of mutual interest and benefit. In addition to supporting the dairy industry by training veterinary students, the program offers specific benefits to investors, including the use of MSU facilities and direct participation in veterinary instruction.     

Subacute atropine toxicity in a pygmy sperm whale, Kogia breviceps.

Atropine, an anticholinergic agent commonly used in human and veterinary medicine, is reported to cause toxicity associated with its antimuscarinic action. A juvenile pygmy sperm whale, Kogia breviceps, was treated with atropine in an attempt to relieve symptoms similar to pyloric stenosis, as has been used in humans. Two doses of 0.01 mg/kg were given i.m., 12 hr apart, followed by three doses of 0.005 mg/kg i.m. s.i.d. over the next 3 days. Symptoms associated with atropine toxicity developed gradually and included hyperexcitability, a generalized ascending paralysis of body musculature, shallow, rapid respiration, vomiting, aspiration of seawater, and pulmonary edema. Treatment with physostigmine salicylate (two doses of 2 mg i.m., I hr apart) was effective in counteracting the paralysis, as well as other symptoms, beginning in as little as 17 min after the first dose, and the whale was back to swimming on its own after 8 hr. All overt symptoms of atropine toxicity were gone in about 24 hr, but there were other possible sequella that lasted much longer.