全文获取类型
收费全文 | 452篇 |
免费 | 48篇 |
专业分类
林业 | 24篇 |
农学 | 10篇 |
基础科学 | 3篇 |
86篇 | |
综合类 | 30篇 |
农作物 | 21篇 |
水产渔业 | 34篇 |
畜牧兽医 | 232篇 |
园艺 | 12篇 |
植物保护 | 48篇 |
出版年
2024年 | 1篇 |
2023年 | 9篇 |
2022年 | 15篇 |
2021年 | 27篇 |
2020年 | 31篇 |
2019年 | 29篇 |
2018年 | 25篇 |
2017年 | 20篇 |
2016年 | 25篇 |
2015年 | 14篇 |
2014年 | 13篇 |
2013年 | 23篇 |
2012年 | 40篇 |
2011年 | 44篇 |
2010年 | 25篇 |
2009年 | 16篇 |
2008年 | 30篇 |
2007年 | 22篇 |
2006年 | 19篇 |
2005年 | 16篇 |
2004年 | 19篇 |
2003年 | 10篇 |
2002年 | 13篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1993年 | 2篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1985年 | 1篇 |
排序方式: 共有500条查询结果,搜索用时 0 毫秒
1.
2.
Alexandra B. Kalamaras Turi K. Aarnes Sarah A. Moore Stephen C. Jones Carolina Ricco Pereira Juan Peng Nina R. Kieves 《Veterinary anaesthesia and analgesia》2021,48(3):415-421
ObjectiveTo compare the quality of postoperative analgesia and sedation after preoperative saphenous and sciatic nerve blockade, preoperative lumbosacral epidural injection and perioperative intravenous (IV) morphine, lidocaine and ketamine infusions in dogs undergoing stifle arthroscopy and tibial plateau leveling osteotomy (TPLO) under general anesthesia.Study designProspective, blinded, randomized, clinical comparison study.AnimalsA total of 45 dogs weighing 33.9 (15.9–56.7) kg and aged 5.2 (1.0–12.0) years, mean (range), undergoing elective unilateral TPLO for spontaneous cranial cruciate ligament rupture.MethodsClient-owned dogs were enrolled. Dogs were randomly assigned to one of three groups: group MLK, perioperative IV morphine, lidocaine and ketamine infusion; group EPID, lumbosacral epidural with ropivacaine and morphine; or group SSNB, saphenous and sciatic nerve blockade with ropivacaine. Routine stifle arthroscopy followed by TPLO surgery was performed. Sedation and pain scores were assessed at 0, 2, 4, 8 and 24 hours following extubation. Rescue analgesia was administered as prescribed by Glasgow composite pain score–short form score >5.ResultsSedation scores for MLK were higher than EPID and SSNB. Pain scores for SSNB were lower than those for EPID and MLK. No significant differences were found in anesthesia duration or surgery duration among groups. No dogs required rescue analgesia.Conclusions and clinical relevanceAlthough analgesia was adequate in all groups, the best combination of analgesia without increased sedation was recorded for SSNB. 相似文献
3.
Alexandra S. Silchenko Anatoly I. Kalinovsky Sergey A. Avilov Pelageya V. Andrijaschenko Roman S. Popov Pavel S. Dmitrenok Ekaterina A. Chingizova Vladimir I. Kalinin 《Marine drugs》2020,18(11)
Six new monosulfated triterpene tetra-, penta- and hexaosides, namely, the kurilosides A1 (1), A2 (2), C1 (3), D (4), E (5) and F (6), as well as the known earlier kuriloside A (7), having unusual non-holostane aglycones without lactone, have been isolated from the sea cucumber Thyonidium (= Duasmodactyla) kurilensis (Levin) (Cucumariidae, Dendrochirotida), collected in the Sea of Okhotsk near Onekotan Island from a depth of 100 m. Structures of the glycosides were established by 2D NMR spectroscopy and HR-ESI mass spectrometry. Kurilosides of the groups A and E contain carbohydrate moieties with a rare architecture (a pentasaccharide branched by C(4) Xyl1), differing from each other in the second monosaccharide residue (quinovose or glucose, correspondingly); kurilosides of the group C are characterized by a unique tetrasaccharide branched by a C(4) Xyl1 sugar chain; and kurilosides of the groups D and F are hexaosides differing from each other in the presence of an O-methyl group in the fourth (terminal) sugar unit. All these glycosides contain a sulfate group at C-6 of the glucose residue attached to C-4 Xyl1 and the non-holostane aglycones have a 9(11) double bond and lack γ-lactone. The cytotoxic activities of compounds 1–7 against mouse neuroblastoma Neuro 2a, normal epithelial JB-6 cells and erythrocytes were studied. Kuriloside A1 (1) was the most active compound in the series, demonstrating strong cytotoxicity against the erythrocytes and JB-6 cells and a moderate effect against Neuro 2a cells. 相似文献
4.
5.
Svenja Hartung Elfi K. Schlohsarczyk Alexandra Jost Marlene Sickinger Kernt Khler 《Journal of veterinary diagnostic investigation》2022,34(2):298
In human and veterinary medicine, mixed Müllerian tumors (MMTs) are rarely diagnosed neoplasms of the tubular female genital tract. Although there are case reports of malignant MMTs in various species, benign MMTs have only been described once in a macaque. Here we present a case of benign MMT in a 12-y-old goat, and review the literature on uterine, cervical, and vaginal neoplasia in goats. The doe was presented with vaginal discharge and was euthanized because of the high suspicion of intraabdominal neoplasia. On gross examination, an ulcerated vaginal mass was identified. Histologically, 2 distinct cell populations were present: smooth muscle cells that were well differentiated and positive for alpha–smooth muscle actin, and ciliated columnar epithelial cells that lined ductal structures and had no signs of malignancy. These findings led to the diagnosis of neoplasia of Müllerian origin. Benign MMT should be considered as a differential diagnosis for uterine and vaginal neoplasms in goats. 相似文献
6.
7.
Computed tomographic diagnosis of necroulcerative reticulorumenitis with portal venous gas in a lamb
Alexandra G. Collins-Webb Robert Bildfell Lauren Newsom 《The Canadian veterinary journal. La revue veterinaire canadienne》2021,62(7):755
A 2-month-old, intact male Merino sheep was presented for acute inappetence and pyrexia. Contrast-enhanced abdominal computed tomography identified reticulorumenal intramural gas with suspected ruminal ulceration, arborizing portal venous gas, and mild peritoneal fluid. The lamb was treated medically for presumptive ulcerative reticulorumenitis and non-septic peritonitis. Over 10 days, the lamb initially showed mild improvement before clinical deterioration and was ultimately euthanized. Definitive diagnosis of necrotizing, ulcerative reticulorumenitis was made via necropsy with histopathology. An underlying cause was not determined.Key clinical message:Contrast-enhanced computed tomography was effective in identifying gastric emphysema and portal venous gas associated with reticuloruminal ulceration. 相似文献
8.
Monika Go?mann Alexandra Scholz Frank Hennig Susanne von Bargen Carmen B��ttner 《Gesunde Pflanzen》2011,63(4):175-182
Fusarium oxysporum and Fusarium proliferatum are important causal agents of crown and root rot of asparagus. In order to detect differences in pathogenicity and aggressiveness, two F. proliferatum and five F. oxysporum single spore isolates from asparagus spears from plantings in Austria and Germany, 55 pure cultures of F. oxysporum from asparagus roots from a planting in Hesse, Germany, and a single F. oxysporum isolate from an asparagus shoot collected in Austria were evaluated in a 28-day quick test on Hoagland??s agar in glass culture tubes. Plantlets were inoculated with spore suspensions from each respective isolate after 14 days of growth under sterile, controlled conditions in a growth chamber. A severity scale was used to assess symptoms on roots two weeks after inoculation. The effects of the single-spore isolates on root and shoot fresh weights of the plantlets were also determined. The pathogenicity of the majority of the F. proliferatum and F. oxysporum isolates included in this study was confirmed. Inoculation with pure and single-spore cultures resulted in elevated disease severity in comparison to non-inoculated controls. In particular, the two F. proliferatum isolates were found to be more aggressive than the F. oxysporum isolates. Moreover, all single spore isolates caused a reduction in fresh weight of roots and shoots in comparison to the controls. With respect to differences among asparagus cultivars, ??Ramos??, was found to be more susceptible than ??Ravel??. Overall, the quick test method was found to be capable of evaluating the pathogenicity and aggressiveness of the tested F. oxysporum and F. proliferatum isolates towards asparagus within 28 days. 相似文献
9.
Benjamin Lopez-Jimena Esther Garcia-Rosado Kim Dawn Thompson Alexandra Adams Carlos Infante Juan Jose Borrego Maria del Carmen Alonso 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(4):355-362
The distribution of red-spotted grouper nervous necrosis virus (RGNNV) antigens was examined by immunohistochemistry in the nervous and non-nervous organs of juvenile European seabass (Dicentrarchus labrax) during the course of an intramuscular infection. Histological changes resulting from the infection were evaluated from 3 days to 2 months post-infection. The specific antibody response was also studied 2 months post-challenge. Viral proteins were present throughout the experimental period in the retina (inner nuclear layer, ganglion layer, outer limiting membrane, and outer plexiform layer), brain (cerebellum and tectum opticum), and liver (hepatocytes and endothelial cells). These proteins were also observed in the renal tubular cells, white pulp of spleen, and in fibroblasts and cartilage of caudal fin. This is the first report of RGNNV proteins appearing in these organs, where the immunostaining was only detected at certain sampling times after the onset of mortality. Brain and retina of virus-exposed fish showed high levels of vacuolation, while accumulation of fat vacuoles was observed in the liver. RGNNV infection also induced a specific antibody response as measured by an ELISA. In summary, this is the first study demonstrating the presence of viral proteins in cells of caudal fin, kidney and spleen of European seabass. 相似文献
10.
Biological control of postharvest diseases of fruit 总被引:1,自引:0,他引:1
Carla Alexandra Nunes 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(1):181-196
Postharvest decay in harvested fruit causes considerable economical losses. Fungicides are the primary means to control these
losses. Public concern in food safety and environmental issues and the increase of pathogen resistant populations have enhanced
the interest in developing alternative methods to fungicides to control postharvest fruit decay. During the last two decades
a huge information and advances concerning the selection of antagonists, mode of action, different approaches to enhance biocontrol
activity, formulation and production have been achieved, and some biofungicides are already in the market. It is likely that
several more products will enter the market in the near future, as the result of the biological control research programs
worldwide. Nonetheless, it is necessary to continue finding new potential microorganisms, better understanding the mode of
action, and pathogen, antagonist and host interactions, to increase the potential of biocontrol helping to become a real alternative
to synthetic postharvest fungicides. This article presents an overview of postharvest biological control approaches and explores
new research possibilities to improve biocontrol activity. 相似文献