不同秋眠级紫花苜蓿茎和叶的碳、氮、磷化学计量特征

为明确不同秋眠级紫花苜蓿品种间营养元素限制性和分配格局的差异性,以国内外收集的10个不同秋眠级紫花苜蓿品种为研究对象,分析其叶片和茎秆的碳(C)、氮(N)、磷(P)含量和化学计量比变化特征。结果表明,紫花苜蓿叶片和茎秆中N和P含量均随着秋眠级的升高呈先降低后升高的趋势,C含量以及C∶N、C∶P和N∶P呈相反的趋势。不同秋眠级苜蓿品种叶片中各元素含量为：秋眠级为4级的新疆大叶苜蓿叶片C含量、秋眠级为9级的WL656HQ叶片N含量均显著大于其他各秋眠级苜蓿品种(P<0.05)。苜蓿茎秆C含量在各秋眠级苜蓿品种间均差异不显著,N、P含量变化与苜蓿叶片相同,且第2茬叶片和茎秆C、N和P含量的变异系数均低于第1茬,两茬表现出相同的规律。除第2茬苜蓿的叶片外,秋眠级对苜蓿叶片和茎秆P含量的影响最大,其次为N含量,对C含量的影响最小。不同茬次间,秋眠级对苜蓿叶片C∶P的影响最大,对N∶P的影响最小。苜蓿叶片C含量与叶片和茎秆C∶N、C∶P呈显著正相关(P<0.05),而与叶片和茎秆N含量呈显著负相关(P<0.05);叶片N、P含量分别与茎秆N、P含量呈显著正相关(P<0.05...     

Diurnal changes in photosynthesis and photosynthetic product partitioning in alfalfa in response to phosphorus application北大核心CSCD

This research explored the effect of different levels of phosphorus application on diurnal variation in photosynthesis and partitioning of photosynthetic products in alfalfa under drip irrigation. In particular, the relationship between photosynthetic product levels in the plant and leaf photosynthetic parameters of alfalfa was clarified. Four phosphorus（P2O5）application levels of 0（CK）,50（P1）,100（P2）and 150 kg·ha-1（P3）were used as treatments in a field experiment. Leaf photosynthetic parameters were measured with a Li-6400 portable photosynthesis instrument on sunny days from 10：00-20：00 during the early flowering stage,together with environment factors and sugar and starch content in the leaves,stems and roots. A feature of the results was a ‘photosynthetic noon-break’phenomenon across all phosphorus application treatments,and stomatal limitation was the main factor responsible for the mid-day decline of net photosynthetic rate （Pn）. Photosynthetically active radiation had the greatest impact on the Pn of alfalfa leaves,while atmospheric CO2 concentration,atmospheric temperature,and atmospheric environmental factors such as relative humidity and vapor pressure deficit based on leaf temp also affected Pn. Principal component analysis showed that alfalfa photosynthetic efficiency in the four P-treatments ranked P2>P1>P3>CK. At the 100 kg·ha-1 phosphorus level（compared to CK）,the hay yield of alfalfa was increased significantly,the soluble sugar contents of leaves,stems,and roots were increased by 11. 6%,5. 0% and 4. 6%,respectively,and the starch contents were increased by 15. 2%,9. 6% and 5. 3%,respectively. Higher overall levels of soluble sugar and starch typically manifested as greater concentration increases in leaves,and relatively smaller increases in roots and stems. The diurnal troughs in Pn,transpiration rate,leaf internal CO2 concentration and water use efficiency（WUE）of alfalfa leaves were less marked in the P treatments,compared with the control treatment. Therefore,appropriate phosphorus application can increase the photosynthetic efficiency of alfalfa leaves,thereby significantly promoting crop growth and development. At the phosphorus（P2O5）application rate of 100 kg·ha-1（P2）,the alfalfa photosynthetic enhancement effect was the most pronounced. © 2022 Editorial Office of Acta Prataculturae Sinica. All rights reserved.