Proteomic profiling of sea bass muscle by two-dimensional gel electrophoresis and tandem mass spectrometry

In this study, the proteome profile of European sea bass (Dicentrarchus labrax) muscle was analyzed using two-dimensional electrophoresis (2-DE) and tandem mass spectrometry with the aim of providing a more detailed characterization of its specific protein expression profile. A highly populated and well-resolved 2-DE map of the sea bass muscle tissue was generated, and the corresponding protein identity was provided for a total of 49 abundant protein spots. Upon Ingenuity Pathway Analysis, the proteins mapped in the sea bass muscle profile were mostly related to glycolysis and to the muscle myofibril structure, together with other biological activities crucial to fish muscle metabolism and contraction, and therefore to fish locomotor performance. The data presented in this work provide important and novel information on the sea bass muscle tissue-specific protein expression, which can be useful for future studies aimed to improve seafood traceability, food safety/risk management and authentication analysis. This work is also important for understanding the proteome map of the sea bass toward establishing the animal as a potential model for muscular studies.     

Effects of water velocity,turbulence and obstacle length on the swimming capabilities of adult Pacific lamprey

The swimming capabilities of adult Pacific lamprey, Entosphenus tridentatus Gairdner, were tested in an experimental vertical‐slot fishway in response to three different fishway features: water velocity (1.2, 1.8 and 2.4 m s^?1), the length of the vertical slot (0.33, 0.66, 1.00 m) and during the presence/absence of a turbulence‐intensifying structure that increased turbulence for each respective treatment. Passage success was lowest (52.7%) and attachment rates were highest (92.9%) during the most difficult passage conditions (high velocity, high turbulence, long slot length). Passage success was >83% for all other treatment combinations. Although passage of the vertical‐slot weir was not associated with fish body size, Pacific lamprey with larger dorsal distances (distance between the two dorsal fins) were more likely to pass the vertical‐slot weir. Increased attachment rates and longer attachment times during strenuous passage conditions suggest that endurance capacity may be an important factor limiting the passage of Pacific lamprey at fishway obstacles.