Alteration of apoptotic susceptibility and bcl-2 gene in malignant transformation of human bronchial epithelial cells

AIM: To demonstrate the susceptibility of cell apoptosis varies during the progress of cell malignant transformation from human being in vitro. METHODS: A SV₄₀T-transfected human bronchial epithelial immortalized cell line (called M) was selected in this work, which has acquired some characteristics of malignant transformation at the later passage. The alterations of apoptosis and bcl-2, P53 genes between early and later passage of M cells were investigated by means of TDT labeling in situ, chromosome FISH, RNA and protein testing, etc. RESULTS: Incidence of apoptosis induced by cis-platin was significantly lower in later than in early passages of M. Levels of bcl-2 mRNA and protein in later passages were higher than early passages of M, and overexpression of bcl-2 was accumulated following the development of cellular malignancy. P53 protein level was as high in early as in later passages. CONCLUSION: Overexpression of bcl-2 decreases the cellular sensitivity to apoptotic inductors plays an important role during progress of carcinogenesis in human bronchial epithelial cancers. The inactivation of P53 protein in the SV₄₀-T transfected M cell line may be one of reasons of bcl-2 overexpression, but not associated with the accumulation of bcl-2 expressed level during cell transformation.     

Youssoufenes A2 and A3, Antibiotic Dimeric Cinnamoyl Lipids from the ΔdtlA Mutant of a Marine-Derived Streptomyces Strain

Two new dimeric cinnamoyl lipids (CL) featuring with an unusual dearomatic carbon-bridge, named youssoufenes A2 (1) and A3 (2), were isolated from the ΔdtlA mutant strain of marine-derived Streptomyces youssoufiensis OUC6819. Structures of the isolated compounds were elucidated based on extensive MS and NMR spectroscopic analyses, and their absolute configurations were determined by combination of the long-range NOE-based ¹H-¹H distance measurements and ECD calculations. Compounds 1 and 2 exhibited moderate growth inhibition against multi-drug-resistant Enterococcus faecalis CCARM 5172 with an MIC value of 22.2 μM.     

梨树在自然水分亏缺下的生理反应

１９９４～１９９６年对梨树在自然水分亏缺和水分充足条件下的主要水分生理参数、气孔相对开张度（ＲＳＯＤ）、蒸腾速率（Ｔｒ）和光合速率（Ｐｎ）进行了比较研究。结果表明，梨树属于低水势耐旱类型，在自然水分亏缺下，其叶水势（ψｗ）显著降低，膨压（ψｐ）明显增高，而ＲＳＯＤ、Ｔｒ和Ｐｎ则无显著变化。梨树具有明显的渗透调节和一定的弹性调节作用，在中度水分亏缺下，其渗透调节能力△π１００或△π０分别为０．４４ＭＰａ和０．７７ＭＰａ，弹性调节能力△｜β｜和△εｍａｘ分别为２．７８和３．１９。另外，在自然水分亏缺下，梨树叶片还具有较强的保水能力，反映在基本无变化的膨压消失点的相对含水量（Ｒ０）、叶表面水分散失率（ＷＬ０）和明显高于ＣＫ的一定ψｗ下的相对含水量（Ｒ）值以及无灌溉园较高的εｍａｘ或｜β｜等方面。     

Bactrian camel (Camelus bactrianus) integrins αvβ3 and αvβ6 as FMDV receptors: Molecular cloning, sequence analysis and comparison with other species

Integrins are heterodimeric adhesion receptors that participate in a variety of cell–cell and cell–extracellular matrix protein interactions. Many integrins recognize RGD sequences displayed on extracellular matrix proteins and the exposed loops of viral capsid proteins. Four members of the αv integrin family of cellular receptors, αvβ3, αvβ6, αvβ1 and αvβ8, have been identified as receptors for foot-and-mouth disease virus (FMDV) in vitro, and integrins are believed to be the receptors used to target epithelial cells in the infected animals. To analyse the roles of the αv integrins from a susceptible species as viral receptors, we have cloned Bactrian camel αv, β3 and β6 integrin cDNAs and compared them to those of other species. The coding sequences for Bactrian camel integrin αv, β3 and β6 were found to be 3165, 2289 and 2367 nucleotides in length, encoding 1054, 762 and 788 amino acids, respectively. The Bactrian camel αv, β3 and β6 subunits share many structural features with homologues of other species, including the ligand binding domain and cysteine-rich region. Phylogenetic trees and similarity analyses showed the close relationships of integrin genes from Bactrian camels, pigs and cattle, which are each susceptible to FMDV infection, that were distinct from the orders Rodentia, Primates, Perissodactyla, Carnivora, Galliformes and Xenopus. We postulate that host tropism of FMDV may in part be related to the divergence in integrin subunits among different species.     

陇东肉牛PON1基因结构与功能的生物信息学分析

为阐明陇东肉牛对氧磷酶1（paraoxonase 1,PON1）基因的结构与功能,本研究运用PCR扩增陇东肉牛PON1基因所有外显子序列并测序,通过Seqman软件完成序列拼接,结合生物信息学方法预测和分析其碱基组成、开放阅读框及编码蛋白的理化性质、原子组成、氨基酸残基数、亲水性和疏水性、分子质量、等电点、二级结构、高级结构等。结果显示,陇东肉牛PON1基因CDS区序列全长1 068 bp,编码355个氨基酸残基,其中数目最多的为亮氨酸（Leu）,数目最少的为半胱氨酸和色氨酸（Cys和Trp）。碱基组成中A+T含量（56.55%）高于G+C含量（43.26%）。与GenBank中牛PON1基因序列（登录号：EU289337）比对分析发现,在PON1基因外显子4、6、8、9中存在突变,但均未导致氨基酸发生变化,为同义突变。PON1蛋白的原子组成是C₁₈₁₀H₂₇₉₆N₄₅₄O₅₃₃S₁₂,分子质量为39.83 ku,理论等电点为5.24,为稳定的水溶性蛋白质。PON1蛋白二级结构中无规则卷曲、延展链、α-螺旋和β-转角分别由159、116、55和25个氨基酸构成,最终形成了以无规则卷曲和延展链为主的混合型。PON1蛋白的疏水性结果表明,第8位苏氨酸（Thr）疏水性最强（最高分值2.878）,第299位脯氨酸（Pro）亲水性最强（最低分值-2.222）。本试验结果为深入研究陇东肉牛PON1蛋白功能及探讨PON1基因突变对甘肃地方肉牛胴体、肉质性状的影响奠定基础。     

猪伪狂犬病的流行病学、临床症状及防控措施

猪伪狂犬病由伪狂犬病毒引起,临床上以妊娠母猪流产、产死胎,新生仔猪出现共济失调、神经症状,并造成仔猪大量死亡为特征.自伪狂犬病毒毒株变异以来,病猪数量不断增加,严重威胁了养猪业健康发展,并造成巨大的经济损失.文章将从流行病学、临床症状、病理变化、鉴别诊断、防控措施等几个方面对猪伪狂犬病进行概述,希望能够对猪伪狂犬病的防...     

PEG介导的玉米弯孢叶斑病菌遗传转化

为了探索不同酶系组成对玉米弯孢叶斑病菌原生质体制备的影响,建立该病菌原生质体遗传转化系统,采用酶系混合物裂解菌丝体制备原生质体,采用PEG介导方法进行原生质遗传转化,通过PCR和Southern blotting技术对转化子进行验证。结果表明:1%溶壁酶+1%蜗牛酶+1%纤维素酶混合酶系为玉米弯孢叶斑病菌原生质体制备的最佳酶系,可以产生原生质体6.78×106 cfu/mL。用PEG介导方法转化共获得16个稳定的转化子,从中随机挑取5个转化子发现质粒pV2已被成功整合到基因组中。本研究获得了制备玉米弯孢叶斑病菌原生质体的最佳酶系,建立了PEG介导的原生质体遗传转化体系,为开展该菌致病相关基因克隆和基因功能研究提供了一种手段。     

"石河子大学农作物害虫标本数据库"的研究与实践

结合本校实际,对石河子大学农作物害虫标本信息进行系统、科学的整理和有序化存储。开发研制出图文并茂的“石河子大学农作物害虫标本数据库”。全库采用国际流行的全息检索技术,具有多种运算方式,检索速度快捷、用户界面友好,是农作物害虫的准确识别与正确防治信息的工具。本数据库的研制与开发对图书馆特色数据库的建设进行了有意义的实践。     

Effects of water‐misting sprays with forced ventilation on post mortem glycolysis,AMP‐activated protein kinase and meat quality of broilers after transport during summer

Effects of water‐misting sprays with forced ventilation on post mortem glycolysis, adenosine monophosphate‐activated protein kinase (AMPK) and meat quality of broilers after transport during summer were investigated in the present paper. A total of 105 mixed‐sex Arbor Acres broilers were divided into three treatment groups: (i) 45 min transport without rest (T); (ii) 45 min transport with 1 h rest (TR); and (iii) 45 min transport with 15 min water‐misting sprays with forced ventilation and 45 min rest (TWFR). Each treatment consisted of five replicates with seven birds each. The results indicated that the water‐misting sprays with forced ventilation could mitigate the stress caused by transport under high temperature conditions during summer, which reduced the energy depletion in post mortem Pectoralis major (PM) muscle. This resulted in a higher energy status compared to the T group, which would decrease the expression of phosphorylation of AMPK (p‐AMPK). Furthermore, decreased the expression of p‐AMPK then slowed down the rate of glycolysis in post mortem PM muscle during the early post mortem period, which in turn lessened the negative effects caused by transport on meat quality. In conclusion, water‐misting sprays with forced ventilation may be a better method to control the incidence of the pale, soft and exudative meat in broilers.     

猪繁殖与呼吸综合征病毒双基因多组合DNA疫苗的构建

本实验根据PRRSV结构蛋白的特性和IFNγ及IL-2这两种细胞因子的特性,构建表达PRRSVGP4-GP5、GP5-M、GP5-N、GP5-IFNγ、GP5-IL-2、N-IFNγ、N-IL-2的重组质粒。这些重组质粒在真核细胞中得到了表达。用这些真核表达质粒可以免疫小猪,通过检测其对猪体免疫应答的诱导能力和接种后动物对PRRSV的抵抗力来对基因疫苗的效能进行研究,以期筛选到有效的基因疫苗并为今后进一步的研究提供思路和理论基础。