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Sakon Sangpradub Arlo W. Fast Somkiat Piyatiratitivorakul Piamsak Menasveta 《Aquaculture International》1994,2(1):49-58
Large pond-reared Penaeus monodon were used in three maturation and spawning trials lasting 60 days. One group of prawns was fed a fresh diet only; a second group was supplied a fresh diet combined with a formulated pelleted diet; a third group of prawns received the formulated diet only. Total maturation (N = 66 and 55) and spawning events (N = 63 and 48) were much greater for prawns receiving only the fresh diet, and the combined fresh with formulated diets, respectively. Prawns receiving only the formulated diet matured only 12 times, and spawned 11 times. Prawns fed a fresh diet produced significantly more eggs than those given a formulated diet alone. No significant differences were seen in eggs produced per spawning event, % fertilization, % hatch or % metamorphosis from egg to protozoea. Female prawns exhibited greater mortality than did males, while females on the fresh diet only had greater mortality than did other females, presumably due to greater handling and other stress factors. Female prawns on a combined diet had lower mortality than other females. The formulated diet used is suitable for supplementation, but not as a sole maturation food. 相似文献
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Bacterial community composition and distribution in different segments of the gastrointestinal tract of wild‐caught adult Penaeus monodon 下载免费PDF全文
Phayungsak Mongkol Phimsucha Bunphimpapha Wanilada Rungrassamee Sopacha Arayamethakorn Sirawut Klinbunga Piamsak Menasveta Sage Chaiyapechara 《Aquaculture Research》2018,49(1):378-392
Bacterial community associated with the gastrointestinal (GI) tract of aquaculture animals can play important roles in health, nutrition and disease. Compared with the GI tract of aquatic vertebrates such as fish, crustacean GI tract has unique structures and surfaces in different segments that may contribute to differences in the bacterial communities. This study examined the bacterial composition and distribution in different segments along the GI tract and in digesta of wild‐caught adult Penaeus monodon using Automated Ribosomal Intergenic Spacer Analysis (ARISA), real‐time quantitative PCR and clone libraries of 16S rRNA genes. Thirty‐nine bacterial species in four phyla including Proteobacteria (α, β, ε, γ), Firmicutes, Bacteroidetes and Actinobacteria were represented in the GI tract of adult P. monodon. Proteobacteria comprised over 80% abundance of the bacterial community in most segments of the GI tract, except the middle intestine that was dominated by Firmicutes (~50% abundance). The results also showed that bacterial communities showed significant differences along the GI tract segments, particularly the hindgut (p < .001) with Vibrio and Ferrimonas as dominant genera. The knowledge about the distribution of bacteria could be useful in understanding interaction of commensal bacteria and pathogens in different segments, and its potential influence on the effectiveness of probiotic bacteria in the GI tract of shrimp. 相似文献
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Piamsak Menasveta Sakon Sangpradub Somkiat Piyatiratitivorakul Arlo W. Fast 《Journal of the World Aquaculture Society》1994,25(1):41-49
The effects of broodstock source (pond-reared and wildcaught from shallow waters) and prawn size (large—weight >120 g, and small-weight < 110 g) on ovarian maturation and reproductive success of Penueus monodon were evaluated. Large females underwent stage IV ovarian maturation and spawned with greater success than did small prawns. Both pond-reared and wildcaught females of the same size exhibited comparable maturation and spawning success. Large females rematured and spawned more frequently than small ones. Total eggs produced by large prawns was significantly greater than for small prawns; nevertheless, prawn source and size had no significant influence on amount of eggs spawned per spawning event. Egg quality varied greatly in terms of percent fertilization, hatch rate, and metamorphosis to first protozwa stage. 相似文献
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Benjamas Chuntapa Sorawit Powtongsook Piamsak Menasveta 《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):355-366
A cyanobacterium (Spirulina platensis) was co-cultured with black tiger shrimp (Penaeus monodon) for water quality control. We evaluated the effects of: (1) three S. platensis trial conditions on inorganic nitrogen concentrations at one shrimp density (S. platensis trial conditions included: absent, nonharvested and semicontinuous harvesting) and (2) two shrimp densities on inorganic nitrogen concentrations, with and without S. platensis. Semicontinuous harvesting of S. platensis at one shrimp density resulted in significantly reduced (P<0.05) inorganic nitrogen concentrations (NH4, NO2 and NO3). With S. platensis absent, ammonium and nitrite concentrations ranged from 0.5 to 0.6 mg l−1, while nitrate concentrations ranged from 16 to 18 mg l−1 by day 44. With nonharvested S. platensis, considerable variability occurred with nitrogen concentrations. Semicontinuous harvest of S. platensis reduced nitrate to 4 mg l−1, while ammonium and nitrite ranged from 0.0 to 0.15 mg l−1, respectively. The factorial evaluation of shrimp density versus presence and absence of S. platensis resulted in greatly reduced nitrogenous compounds with S. platensis present regardless of shrimp density, and only moderately increased nitrogen with greater shrimp density. Without S. platensis, all nitrogen compounds were substantially elevated and shrimp survived was significantly reduced at high shrimp density. 相似文献
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Sirithorn Janpoom Pornthip Sawatpanich Sirawut Klinbunga Piamsak Menasveta Bavornlak Khamnamtong 《Fisheries Science》2017,83(4):515-522
Molecular markers that allow selection of juveniles and broodstock with a high growth performance are useful for the aquaculture industry. In this study, association between single nucleotide polymorphisms (SNPs) of the Activin type IIB receptor (LcActRIIB) and growth-related parameters of Asian seabass Lates calcarifer juveniles were examined by PCR-direct sequencing. Three SNPs (G>Ac.965+g.7, C>A>T c.965+g.33 and G>A c.965+g.189) generating seven composite SNP genotypes (diplotypes) were found in the LcActRIIB gene segment (398 bp). Among three identified SNPs, only a G>Ac.965+g.7 SNP showed significant association with growth traits (average body weight, total length, hepatic weight and hepatosomatic index) of examined fish (P < 0.01). Significant association between diplotypes and growth-related parameters were found. Juveniles exhibiting diplotypes I–V showed greater growth-related parameters than those exhibiting diplotypes VI (P < 0.001; N = 57). Simple methods for rapid genotyping of a G>Ac.965+g.7 SNP were successfully developed based on gel-based bi-allelic PCR amplification of specific alleles (Bi-PASA) and real-time PCR-based PASA analyses (N = 99). Identical genotypes were consistently obtained from different detection methods. 相似文献
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