Simultaneous Extraction and Fractionation of Fish Oil from Tuna By-Product Using Supercritical Carbon Dioxide (SC-CO2)

ABSTRACT

Fish oil was extracted and simultaneously collected into six fractions based on molecular weight and the chain length of triglycerides in terms of fatty acid constituents without splitting of the triglycerides, using supercritical carbon dioxide (SC-CO₂) at optimized conditions of 40 MPa, 65°C, and a flow rate 3 mL min^?1. In each type of fractionation, the first fraction (F1) was rich in saturated fatty acids (SFA; 52.57 to 61.26%), followed by monounsaturated fatty acids (MUFA; 22.17 to 23.22%) and polyunsaturated fatty acids (PUFA; (0.54 to 20.37%); the sixth fraction (F6) was rich in PUFA (48.93%), followed by MUFA (33.59%) and SFA (13.61%). It was obvious that short-chain fatty acids were extracted at an earlier fraction; therefore, the latter fractions were dominant in long-chain fatty acids, especially MUFA and PUFA. Thus, omega-3 fish oil (last three fractions) was successfully separated to be used as a value-added health product.     

The conservation of the endangered Mediterranean common dolphin (Delphinus delphis): Current knowledge and research priorities

1. Preservation of endangered species is now recognized as a relevant component of regional and global conservation actions achieved through the maintenance of the remaining populations in a favourable conservation status. The Mediterranean common dolphin (Delphinus delphis) is listed as an Endangered subpopulation in the International Union for Conservation of Nature Red List, with declining numbers in some of the areas where it has been studied for numerous years. Prey depletion, fisheries bycatch, pollution, health risks, and climate change are among the recognized threats that the species is facing in the region.
2. The existing national, regional, and international legislation pertinent to Mediterranean countries should aid greater science–policy–management integration and support innovative research and monitoring towards effective conservation. The challenges inherent in studying the pelagic D. delphis should not preclude the use of long-term, year-round, multiplatform surveys, side by side with the latest molecular tools, which aid our understanding of its populations' genetic diversity, resistance to diseases, and resilience in a semi-enclosed fast-changing sea.
3. Addressing the multiplicity of problems confronting the species requires collaborative effort at all levels to share and merge resources, data, and expertise more efficiently. In this review, we provide an overview of the current knowledge on the Mediterranean common dolphin, the main threats, and knowledge gaps that are priorities for addressing its conservation, while providing clear recommendations for effectively safeguarding the species and its habitat at both local and regional levels.

     

Minimizing ammonia volatilization from urea,improving lowland rice (cv. MR219) seed germination,plant growth variables,nutrient uptake,and nutrient recovery using clinoptilolite zeolite

The anionic nature and high cation exchange capacity (CEC) of clinoptilolite zeolite can be exploited to reduce ammonia (NH₃) loss from urea and to improve soil chemical properties to increase nutrient utilization efficiency in lowland rice cultivation. A closed-dynamic airflow system was used to determine NH₃ loss from treatments (20, 40, and 60 g clinoptilolite zeolite pot^?1). Seed germination study was conducted to evaluate the effects of clinoptilolite zeolite on rice seed germination. A pot study was conducted to determine the effects of clinoptilolite zeolite on rice plant growth variables, nutrient uptake, nutrient recovery, and soil chemical properties. Standard procedures were used to determine NH₃ loss, rice plant height, number of leaves, number of tillers, dry matter production, nutrient uptake, nutrient recovery, and soil chemical properties. Application of clinoptilolite zeolite (15%) increased shoot elongation of seedlings and significantly reduced NH₃ loss (up to 26% with 60 g zeolite pot^?1), and increased number of leaves, total dry matter, nutrient uptake, nutrient recovery, soil pH, CEC, and exchangeable Na⁺. Amending acid soils with clinoptilolite zeolite can significantly minimize NH₃ loss and improve rice plant growth variables, nutrient uptake, nutrient recovery, and soil chemical properties. These findings are being validated in our ongoing field trials.     

Enhancement of protective immunity in blue gourami,Trichogaster trichopterus (Pallas), against Aeromonas hydrophila and Vibrioanguillarum by A. hydrophila major adhesin

Blue gourami, Trichogaster trichopterus (Pallas), were intraperitoneally immunized with major adhesin, a 43 kDa OMP protein isolated from fish Aeromonas hydrophila, in the presence of Freund's complete adjuvant (FCA). Three weeks later, a booster injection of adhesin without FCA was administered. Control group fish were similarly treated with phosphate‐buffered saline (PBS) and FCA. Results showed that anti‐adhesin serum obtained from fish after booster immunization exhibited very strong ability in agglutinating bacterial cells. Although this antiserum had no bactericidal effect, it could significantly inhibit serologically different strains of A. hydrophila from invading EPC (Epithelioma papillosum of carp) cells in vitro. In addition, the proliferative response of head kidney leucocytes of these immune fish was significantly increased as compared to that of the control. The results also showed that the major adhesin could provide significant protective immunity to fish against the challenge by homologous and heterologous strains of A. hydrophila and one virulent strain of Vibrio anguillarum.     

含气率对小流量下混流式混输泵轴系振动的影响

振动是反映混输泵运行稳定性的关键指标,液体含气率对混输泵的振动强度有重要影响.为探究小流量工况下含气率变化对混输泵轴系振动的影响规律,该研究以某3级混流式气液混输泵为研究对象,采用建立的多通道振动测试系统采集小流量工况下混输泵输送不同含气率的水时主轴和轴承座的振动信号,然后分析水中含气率对主轴和轴承座振动特性的影响规律...     

Reducing Soil Phosphorus Fixation to Improve Yield of Maize on a Tropical Acid Soil Using Compost and Biochar Derived from Agro-Industrial Wastes

Phosphorus is an essential element required to maintain profitable crop production. Most soils of the tropics, such as Ultisols, are acidic and fix phosphorus because of their characteristically high contents of aluminium and iron. Compost and biochar could be used to mitigate phosphorus fixation by reducing the phosphorus sorption sites. This study aimed to: (i) improve soil phosphorus availability, nutrient uptake, and yield of maize using biochar and pineapple leaf residue compost; and (ii) determine if the use of biochar and compost could exert a residual effect on phosphorus nutrition in the second cycle of the field trial. Field trials were carried out using a Zea mays L. hybrid as the test crop. At harvest, the plants were harvested, partitioned into leaves and stems, and analyzed. Soil samples were also collected and analyzed. Ears were harvested to determine the yield from each treatment. The results suggest that the soil total phosphorus and available phosphorus recovered from the treatments with the organic amendments were higher compared with the non-organic amendments. The availability of soil nutrients (nitrogen, potassium, calcium, magnesium, and sodium) in the soils and yield of maize were higher in the treatments with the organic amendments in the first and second field trials. These results further confirm that amending chemical fertilizers with organic amendments have a larger residual effect than chemical fertilizers only. Amending chemical fertilizers with organic amendments can be used to ameliorate phosphorus fixation of acid soils to improve maize production on acid soils.     

The role of soils in the regional carbon budget of pine forests in Karelia

The carbon pool and its fluxes were studied in Karelian pine forests of automorphic sites for the characterization of the regional carbon cycle. On the permanent sample plots arranged in pine stands (dominant forest types) of the middle and northern taiga, the structure and dynamics of the phytomass, as well as the composition of the soil organic matter, were investigated. The contribution of different components of the forest ecosystems to the regional carbon budget was studied. The carbon pools in the soils of the middle and northern taiga subzones were 330–440 × 10² and 440–550 × 10² kg/ha, respectively. The share of soil carbon in the total carbon pool of the forest biogeocenoses studied amounted to 46–35 and 33–24%, respectively. The period of considerable carbon fixation in the pine ecosystem (nearly 20 × 10² kg/ha/yr) turned out to be limited to the middle-aged stands. In the mature and overmature pine forests, only 4 × 10² kg of C/ha/yr were fixed. The main part of carbon in the soil (65–75%) was contained in the illuvial horizons, although its content there was less that 1% as compared to 30–50% in the forest litters. On industrial harvesting of wood, the main reserves of soil carbon are not spent; only the fund of mobile carbon in organic horizons of poor soils is exhausted or carbon accumulates intensely due to the development of continuous plant cover on fertile soils.     

Genetic differentiation between fake abalone and genuine Haliotis species using the forensically informative nucleotide sequencing (FINS) method

Abalones ( Haliotis species) are a popular delicacy and commonly preserved in dried form either whole or in slices or small pieces for consumption in Asian countries. Driven by the huge profit from trading abalones, dishonest traders may substitute other molluscan species for processed abalone, of which the morphological characteristics are frequently lost in the processed form. For protection of consumer rights and law enforcement against fraud, there is a need for an effective methodology to differentiate between fake and genuine abalone. This paper describes a method (validated according to the international forensic guidelines provided by SWGDAM) for the identification of fake abalone species using forensically informative nucleotide sequence (FINS) analysis. A study of the local market revealed that many claimed "abalone slice" samples on sale are not genuine. The fake abalone samples were found to be either volutids of the genus Cymbium (93%) or the muricid Concholepas concholepas (7%). This is the first report of Cymbium species being used for the preparation and sale as "abalone" in dried sliced form in Hong Kong.