The Production and Some Properties of the Brown Pigment of Aeromonas Liquefaciens

The optimum conditions for pigment production by Ae. liquefaciens was found to be 30 °C and aerobic incubation, and the properties of the pigment are shown to be similar to those of the synthetic dopa-melanin referred to. The enzyme phenol oxidase was demonstrated in the culture filtrates, but not in extracts of disintegrated cells. As precursors for the pigment production, which was shown to be strictly pH dependent, DL-tyrosine, DL-dopa and catechol were used with success, while numerous other amino acids failed. The significance of the pigment as a criterium for the identification of strains which are pathogenic to certain animals or fishes, is not known.     

Digestion of lipids and carbohydrates from microalgae (Chaetoceros muelleri Lemmermann and Isochrysis aff. galbana clone T‐ISO) in juvenile scallops (Pecten maximus L.)

The digestion of laminaran and neutral lipids from the microalgae Chaetoceros muelleri or mixtures of C. muelleri and Isochrysis galbana clone T‐ISO in 4‐mm scallops Pecten maximus were studied. The microalgae were cultivated at fixed low and high growth rate (5% and 60% of μ_max). The cultivation method clearly affected the lipid and carbohydrate contents, as well as their enzyme activity. Chaetoceros muelleri and T‐ISO grown at low growth rate exhibited a considerably higher content of lipids and carbohydrates than those algae grown at high growth rate. The specific activity of laminaranase and neutral lipase was the highest in C. muelleri grown at the low growth rate. Growth rate of scallops was best when they were fed a mono‐species diet of C. muelleri grown at low growth rate. The growth rate of the scallops was positively correlated to the dietary carbohydrate content as well as to the specific activity of neutral lipase and laminaranase in the diet. The capacity to digest carbohydrates (laminaran) might be limiting for the young scallops, and the content of carbohydrates and enzymatic activities of laminaran in the diet should be considered when formulating an optimal diet, both when the diet consist of live microalgae and formulated diets.     

Metabolic profiles in five high-producing Swedish dairy herds with a history of abomasal displacement and ketosis

Background

Body condition score and blood profiles have been used to monitor management and herd health in dairy cows. The aim of this study was to examine BCS and extended metabolic profiles, reflecting both energy metabolism and liver status around calving in high-producing herds with a high incidence of abomasal displacement and ketosis and to evaluate if such profiles can be used at herd level to pinpoint specific herd problems.

Methods

Body condition score and metabolic profiles around calving in five high-producing herds with high incidences of abomasal displacement and ketosis were assessed using linear mixed models (94 cows, 326 examinations). Cows were examined and blood sampled every three weeks from four weeks ante partum (ap) to nine weeks postpartum (pp). Blood parameters studied were glucose, fructosamine, non-esterified fatty acids (NEFA), insulin, β-hydroxybutyrate, aspartate aminotransferase, glutamate dehydrogenase, haptoglobin and cholesterol.

Results

All herds had overconditioned dry cows that lost body condition substantially the first 4–6 weeks pp. Two herds had elevated levels of NEFA ap and three herds had elevated levels pp. One herd had low levels of insulin ap and low levels of cholesterol pp. Haptoglobin was detected pp in all herds and its usefulness is discussed.

Conclusion

NEFA was the parameter that most closely reflected the body condition losses while these losses were not seen in glucose and fructosamine levels. Insulin and cholesterol were potentially useful in herd profiles but need further investigation. Increased glutamate dehydrogenase suggested liver cell damage in all herds.     

Influence of low phosphorus intake during early lactation on apparent digestibility of phosphorus and bone metabolism in dairy cows

In this study, the effects of reducing the dietary phosphorus (P) supply during the first 4 months of lactation on P balance and bone metabolism were investigated in dairy cows during a full lactation, including the dry period. Twenty-two multiparous cows of the Swedish Red and White Breed were included in the experiment. Eleven cows received a dietary P concentration of 0.43% of DM during the whole lactation (NP), and 11 cows received a dietary P concentration of 0.32% of DM during the first 4 months of lactation, followed by 0.43% of DM during the rest of lactation (LP). Total collection of faeces was carried out during five different stages of lactation, for 5 consecutive days at each occasion. The dynamics of bone metabolism was investigated by monitoring one bone-formation marker, identifying osteocalcin (OC), and one bone-resorption marker identifying C-telopeptide fragments of collagen type I (CTx) in blood plasma. At the two first collection periods, 3–7 and 11–15 weeks after parturition, the apparent digestibility of P was higher in the LP cows (52%) than in the NP cows (42%). During the following collection periods, no difference in the apparent digestibility could be noted between the two groups of cows. Phosphorus retention did not differ between treatments at any collection period. The highest retention was observed during late lactation and during the dry period. The profiles of the bone metabolism markers indicated a net resorption of bone during early lactation, but there were no differences in marker concentrations between the groups during early lactation, indicating that the LP diet did not induce a further elevated net bone resorption.     

Identifying the biological effects of pre-commercial thinning on diameter growth in young Scots pine stands

Data from seven Scots pine (Pinus sylvestris L.) stands in central Sweden that had undergone pre-commercial thinning (PCT) were used to investigate the biological effect of PCT on the diameter at breast height (DBH) growth of the remaining trees. Two treatments were considered: a PCT treatment and a control (C) with no PCT. The DBH of the trees in each stand was measured on up to four occasions over 15 years. We examined mean DBH and DBH growth of the largest 300, 600, 900 and 1200 trees ha^?1. Two methods of selecting the trees used to calculate the mean DBH values for each measurement occasion were considered: the actual mean DBH (Dma), which is based on the DBH distribution of the trees on the measurement occasion in question, and the genuine mean DBH (Dmg), which is based on the DBH distribution of the trees on the final measurement occasion of the study and therefore focuses on the same set of trees for all measurement occasions. There was no clear difference between the Dma- and Dmg-based DBH increments, but the Dmg values tended to be somewhat larger both for the C and PCT treatments. Over a 15-year period, the relative mean yearly increments (Dma/Dmg) for different tree size classes ranged from 0.91 to 0.98, with lower values for the C treatment and larger DBH size classes. We found that PCT promotes DBH growth: over a 15-year period, the increased growth amounted to about 2.0 mm year^?1 compared to the trees in unthinned plots.     

Impacts from Deposition on Swedish Forest Ecosystems Identified by Integrated Monitoring

Integrated monitoring of ecosystems (IM) is an international co-operative programme (ICP) to control effects of air pollution and climate change on water, soil and biological systems. It is a part of the Convention on Long-Range Transboundary Air Pollution (CLRTAP) of the United Nations Economic Commission of Europe (UN/ECE). The ICP-IM is undertaken on sites/catchments to investigate acidification, eutrophication and heavy metals with an integrated approach. In Sweden, long-term time series from forest ecosystems, with a long and stable continuity, will reveal trends and changes in processes and enable modelling to be undertaken. Investigations of acidity/alkalinity in relation to mineral and organic acids indicated the importance of atmospheric deposition. Recent results show very high inorganic nitrogen retention (99%), a net loss of sulphur originating mainly from organic horizons, and a high inorganic aluminium content in the illuvial soil horizons which could be detrimental to forests. Forest deficiency could also be caused by an observed ongoing translocation of Zn to deeper soil layers implying a movement towards increased release to surface waters.     

Metal fluxes to Swedis forest lakes

Data on atmospheric deposition, transport via run-off water and contents in soil were used to estimate the fluxes of Zn, Cd, Cu, Pb, and Hg to Swedish forest lakes. The calculations refer to a hypothetical lake with a surface area of about 9% of the total catchment area. There are clear differences in the pathways and transport mechanisms for the different elements. The dominating pathway for the input of Zn and Cd to lakes is via run-off from the drainage area. About 60 to 95% of the total load comes from run-off, highest in the acidified areas. The acidification status of the soil is by far the most important factor regulating the mobility of these elements. The amounts of Hg and Pb deposited on the drainage area are mainly accumulating in the surficial soil layers. The transport of these elements is primarily associated with humic substances. About 30 to 50% of the total load of Pb and 25 to 75% of the total load of Hg to lakes originates from run-off. The transport mechanisms for Cu are similar to those of Pb and Hg, but due to the lower anthropogenic airborne load, the direct deposition is of less importance.     

Determination of total folate in plant material by chemical conversion into para-aminobenzoic acid followed by high performance liquid chromatography combined with on-line postcolumn derivatization and fluorescence detection

A procedure involving chemical conversion of all forms of folate present in plant material into para-aminobenzoic acid (PABA) and a liquid chromatographic-fluorimetric determination with on-line postcolumn derivatization is reported. All folates are cleaved with liberation of PABA by hydrogen peroxide followed by acid hydrolysis using concentrated hydrochloric acid (37%) at 110 degrees C for 6 h. The reaction yield for individual folates conversion to PABA ranged from 44.4 to 97.3%. PABA could be determined sensitively by on-line postcolumn derivatization with fluorescamine, the detection limit for PABA being 3.02 nM. On the basis of this principle, a method for the determination of total folate in plant material, including a purification step on an affinity column, is presented, which offers a sufficient sensitivity and selectivity for routine analysis of total folate in natural samples. The total folate contents of tomatoes, carrots, white cabbage, and spinach were determined, and the results were quite comparable to the data reported. The recovery of PABA and the comparison of total folate analysis in spinach on different occasions (over 6 months) are also reported. The method is reliable, universal for all folates, including polyglutamate and monoglutamate forms, and eliminates the need for a deconjugation step and multiple conversion reactions.     

Effects of maturity stage and feeding strategy of whole crop barley silage on intake, digestibility and milk production in dairy cows

Five diets containing concentrate, grass silage and whole crop barley silage (WCBS) harvested at different maturity stages were fed to 15 multiparous dairy cows in an incomplete change-over design over three periods. Three diets contained 10.7 kg dry matter (DM) concentrate, 4 kg DM grass silage, and ad libitum access to WCBS harvested at either the heading stage (B1), the early milk stage (B2) or the early dough stage (B3) of maturity. The other two diets contained 10.7 kg DM concentrate, whereas grass silage and WCBS at heading were mixed at two different ratios with a DM content of WCBS of either 0.30 (M1) or 0.70 (M2), and the mixtures were fed ad libitum. Intakes of DM (kg day^− 1: B1 = 21.0, B2 = 20.6 and B3 = 20.0) and neutral detergent fibre (NDF; kg day^− 1: B1 = 7.0, B2 = 6.4 and B3 = 6.3) decreased, whereas starch intake increased (kg day^− 1: B1 = 3.1, B2 = 3.5 and B3 = 4.0) with increasing maturity at harvest. The apparent organic matter (OM) digestibility (g kg^− 1: B1 = 800, B2 = 774 and B3 = 729) decreased with increasing maturity stage, and consequently so did the digestible OM intake. Milk (kg day^− 1: B1 = 27.2, B2 = 26.1 and B3 = 25.9) and energy corrected milk (ECM; kg day^− 1: B1 = 31.0, B2 = 29.4 and B3 = 28.2) yields, and protein concentration (g kg^− 1: B1 = 37.1, B2 = 36.4 and B3 = 36.0) decreased with increasing maturity stage of the WCBS. When cows were fed diet B3 the milk fat concentration decreased (46.4 g kg^− 1) compared to diets B1 (49.3 g kg^− 1) and B2 (49.4 g kg^− 1). The difference in ECM yield between diets B1 and B3 was due to a combined effect of lower milk yield, and lower protein and fat concentrations. This was caused by the higher starch and lower NDF intakes with diet B3, which decreased the milk fat concentration. Moreover, a lower energy intake of diet B3 due to lower OM digestibility decreased milk protein concentration and milk yield. Mixing WCBS at the heading stage with grass silage (M2) decreased digestibility, compared to feeding the forages separately (B1). However, the differences were small and may be a result of soil contamination at harvest of some of the silages, which made the method with using incomplete faecal collection and internal marker acid insoluble ash less reliable. Including WCBS at the heading stage at 0.30 or 0.70 of forage DM did not affect DM intake or diet digestibility, probably because the grass silage in the study was very similar in energy content to the WCBS harvested at heading.     

Blood parameters in Swedish dairy herds with high or low incidence of displaced abomasum or ketosis

Sixty dairy herds were studied to investigate the association between long-term incidence of displaced abomasum and clinical ketosis and body condition score and blood profiles, including parameters estimating energy metabolism and hepatic lipidosis in the periparturient period and early lactation. Blood samples were taken around parturition and in early lactation from cows without apparent clinical symptoms of metabolic disorders. A difference in metabolism between high and low incidence herds was shown post-partum by a lower metabolic index (the revised Quantitative Insulin Sensitivity Check Index, RQUICKI), and tendencies for higher concentrations of glucose, insulin and non-esterified fatty acids in the high incidence herds. High incidence herds had more cows and produced on average 1400kg energy-corrected milk per cow per year more than the low incidence herds. No differences were found in parameters reflecting liver cell damage. In the first 3weeks post-partum the RQUICKI was a more sensitive marker of herds with a high incidence of displaced abomasum and clinical ketosis than any of the individual parameters, but further research is needed before practical applications of the RQUICKI can be foreseen.